A MODIFIED SPECTROPHOTOMETRIC DETERMINATION OF CHYMOTRYPSIN, TRYPSIN, AND THROMBIN

Canadian Science Publishing - Tập 37 Số 12 - Trang 1393-1399 - 1959
Brian C. W. Hummel1
1Food and Drug Laboratories, Department of National Health and Welfare, Ottawa, Ontario

Tóm tắt

The spectrophotometric procedure proposed by Schwert and Takenaka for the assay of chymotrypsin and trypsin has been modified and extended to include the application to N-benzoyl-L-tyrosine ethyl ester and α-p-toluenesulphonyl-L-arginine methyl ester. The greater degree of sensitivity and specificity thus achieved permits the determination of traces of chymotrypsin in the presence of relatively large amounts of trypsin and vice versa. A similar spectrophotometric procedure for the assay of thrombin is described.

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Tài liệu tham khảo

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Thông tin
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Canadian Science Publishing

Tập 37 Số 12

1393-1399

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