A MAP Kinase Targeted by Endotoxin and Hyperosmolarity in Mammalian Cells

American Association for the Advancement of Science (AAAS) - Tập 265 Số 5173 - Trang 808-811 - 1994
Jiahuai Han1, Jeffrey Lee, L. Bibbs, R. J. Ulevitch
1Department of Immunology, Scripps Research Institute, La Jolla, CA 92037

Tóm tắt

Mammalian cells respond to endotoxic lipopolysaccharide (LPS) by activation of protein kinase cascades that lead to new gene expression. A protein kinase, p38, that was tyrosine phosphorylated in response to LPS, was cloned. The p38 enzyme and the product of the Saccharomyces cerevisiae HOG1 gene, which are both members of the mitogen-activated protein (MAP) kinase family, have sequences at and adjacent to critical phosphorylation sites that distinguish these proteins from most other MAP kinase family members. Both HOG1 and p38 are tyrosine phosphorylated after extracellular changes in osmolarity. These findings link a signaling pathway in mammalian cells with a pathway in yeast that is responsive to physiological stress.

Từ khóa


Tài liệu tham khảo

10.1016/0092-8674(91)90098-J

10.1126/science.7681220

CORRADIN, S.B., ENHANCEMENT OF MURINE MACROPHAGE BINDING OF AND RESPONSE TO BACTERIAL LIPOPOLYSACCHARIDE (LPS) BY LPS-BINDING PROTEIN, JOURNAL OF LEUKOCYTE BIOLOGY 52: 363 (1992).

DAVIS, R.J., THE MITOGEN-ACTIVATED PROTEIN-KINASE SIGNAL-TRANSDUCTION PATHWAY, JOURNAL OF BIOLOGICAL CHEMISTRY 268: 14553 (1993).

10.1016/0092-8674(94)90380-8

10.1126/science.1970196

FERNANDEZ, J, INTERNAL PROTEIN-SEQUENCE ANALYSIS - ENZYMATIC DIGESTION FOR LESS THAN 10 MU-G OF PROTEIN-BOUND TO POLYVINYLIDENE DIFLUORIDE OR NITROCELLULOSE MEMBRANES, ANALYTICAL BIOCHEMISTRY 201: 255 (1992).

GLAUSER, M.P., SEPTIC SHOCK - PATHOGENESIS, LANCET 338: 732 (1991).

GLENNEY, J.R., ISOLATION OF TYROSINE-PHOSPHORYLATED PROTEINS AND GENERATION OF MONOCLONAL-ANTIBODIES, METHODS IN ENZYMOLOGY 201: 92 (1991).

GONZALEZ, F.A., HETEROGENEOUS EXPRESSION OF 4 MAP KINASE ISOFORMS IN HUMAN TISSUES, FEBS LETTERS 304: 170 (1992).

GUSTIN M COMMUNICATION.

HAN J unpublished data.

HAN, J.H., ENDOTOXIN INDUCES RAPID PROTEIN-TYROSINE PHOSPHORYLATION IN 70Z/3 CELLS EXPRESSING CD14, JOURNAL OF BIOLOGICAL CHEMISTRY 268: 25009 (1993).

10.1126/science.3291115

10.1126/science.8385802

LEE, J.D., GLYCOSYL-PHOSPHATIDYLINOSITOL-ANCHORED OR INTEGRAL MEMBRANE FORMS OF CD14 MEDIATE IDENTICAL CELLULAR-RESPONSES TO ENDOTOXIN, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 90: 9930 (1993).

MANTHEY, C.L., TAXOL INCREASES STEADY-STATE LEVELS OF LIPOPOLYSACCHARIDE-INDUCIBLE GENES AND PROTEIN-TYROSINE PHOSPHORYLATION IN MURINE MACROPHAGES, JOURNAL OF IMMUNOLOGY 149: 2459 (1992).

NISHIDA, E, THE MAP KINASE CASCADE IS ESSENTIAL FOR DIVERSE SIGNAL TRANSDUCTION PATHWAYS, TRENDS IN BIOCHEMICAL SCIENCES 18: 128 (1993).

SCHILD, D, CLONING OF 3 HUMAN MULTIFUNCTIONAL DENOVO PURINE BIOSYNTHETIC GENES BY FUNCTIONAL COMPLEMENTATION OF YEAST MUTATIONS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 87: 2916 (1990).

TILLY, B.C., PROTEIN-TYROSINE PHOSPHORYLATION IS INVOLVED IN OSMOREGULATION OF IONIC CONDUCTANCES, JOURNAL OF BIOLOGICAL CHEMISTRY 268: 19919 (1993).

TRACEY, K.J., ADV SURG 23: 21 (1990).

ULEVITCH, R. J., ADVANCES IN IMMUNOLOGY, VOL 53 53: 267 (1993).

ULEVITCH, R.J., RECOGNITION OF ENDOTOXIN BY CELLS LEADING TO TRANSMEMBRANE SIGNALING, CURRENT OPINION IN IMMUNOLOGY 6: 125 (1994).

WEINSTEIN, S.L., BACTERIAL LIPOPOLYSACCHARIDE INDUCES TYROSINE PHOSPHORYLATION AND ACTIVATION OF MITOGEN-ACTIVATED PROTEIN-KINASES IN MACROPHAGES, JOURNAL OF BIOLOGICAL CHEMISTRY 267: 14955 (1992).

WEINSTEIN, S.L., BACTERIAL LIPOPOLYSACCHARIDE STIMULATES PROTEIN TYROSINE PHOSPHORYLATION IN MACROPHAGES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 4148 (1991).

10.1038/367704a0

Thông tin
Thông tin xuất bản

American Association for the Advancement of Science (AAAS)

Tập 265 Số 5173

808-811

Thông tin tác giả