A Comparison of in vitro Culture of Fetal Nucleated Erythroblasts from Fetal Chorionic Villi and Maternal Peripheral Blood for Noninvasive Prenatal Diagnosis

Fetal Diagnosis and Therapy - Tập 32 Số 3 - Trang 194-200 - 2012
Shengxia Zheng1, Xianhong Tong1, Li‐Min Wu1, Guoping He2, Ding Bangsheng3, Li‐Juan Yao3, Yusheng Liu1
1Center for Reproductive Medicine
2Laboratory of Medical Genetics, and
3Laboratory of Hematology, Anhui Medical University Affiliated Provincial Hospital, Hefei, PR China

Tóm tắt

<b><i>Objective:</i></b> We tested the feasibility of the in vitro culture of fetal nucleated erythroblasts from maternal blood for noninvasive prenatal screening as a substitute for culturing fetal nucleated erythroblasts from fetal villi. <b><i>Method:</i></b> Nucleated blood cells separated via Percoll from 52 samples of fetal villi and maternal peripheral blood were cultured with or without magnetic-activated cell sorting glycophorin A (MACS-GPA+), and detected by an anti-hemoglobin-epsilon (FITC) antibody. Gender of the epsilon-positive cells were identified by FISH and further confirmed by PCR of the villi karyotype. Developmental stages of nucleated erythroblasts from villi and blood with MACS-GPA+ were analyzed by Wright-Giemsa staining. <b><i>Results:</i></b> In the maternal blood, epsilon-positive cells were found in 4 and 24 cultured samples with and without MACS-GPA+, respectively. Also, Y-signals were visualized in 3 out of 4 and in 15 out of 24 cases in the epsilon-positive cells. Although epsilon-positive cells were found in all villus samples irrespective of MACS-GPA+ sorting, Y-signals were visualized in 31 out of 52 cases. Proerythroblasts and basophilic erythroblasts occupied 7 and 1% in fetal and maternal samples (with MACS-GPA+), respectively. <b><i>Conclusions:</i></b> The in vitro culturing of fetal nucleated erythroblasts from maternal blood is not feasible with the current techniques for prenatal diagnosis, because the fetal nucleated erythroblast is not well developed in vitro. This may be attributed to the low proportion of these erythroblasts at an early stage in the fetal circulation and the low permeability of these cells to the maternal blood.

Từ khóa


Tài liệu tham khảo

10.1016%2FS1028-4559%2809%2960184-4

10.1364%2FOL.34.001483

10.1073%2Fpnas.93.2.705

10.1002%2F%28SICI%291097-0223%28199709%2917%3A9%3C801%3A%3AAID-PD153%3E3.0.CO%3B2-E

10.1002%2Fpd.1970141308

10.1086%2F514889

10.1046%2Fj.1365-2141.1999.01383.x

10.1155%2F2009%2F659219

10.1002%2Fpd.1199

10.1016%2Fj.jim.2010.09.039

10.1034%2Fj.1399-0004.2001.590202.x

10.1002%2Fpd.222

10.1002%2F%28SICI%291097-0320%2819960901%2925%3A1%3C37%3A%3AAID-CYTO5%3E3.0.CO%3B2-B

10.1016%2FS0002-9378%2899%2970622-8

10.1016%2FS0014-5793%2897%2900144-0

10.1007%2FBF00276341

10.1016%2FS0140-6736%2894%2993031-7

10.1002%2F%28SICI%291097-0223%28199612%2916%3A12%3C1073%3A%3AAID-PD38%3E3.0.CO%3B2-D

10.1016%2FS1079-9796%2803%2900008-1

10.1002%2F%28SICI%291097-0223%28199809%2918%3A9%3C883%3A%3AAID-PD365%3E3.3.CO%3B2-%23

10.1002%2F1097-0223%28200006%2920%3A6%3C479%3A%3AAID-PD861%3E3.0.CO%3B2-8

10.1002%2F1096-8628%2820010215%2999%3A1%3C34%3A%3AAID-AJMG1106%3E3.0.CO%3B2-D

10.1167%2Fiovs.05-0343

10.1002%2Fpd.1456

10.1002%2F%28SICI%291097-0223%28199906%2919%3A6%3C521%3A%3AAID-PD578%3E3.0.CO%3B2-N

10.1093%2Fmolehr%2Fgag027

10.1002%2F1097-0223%28200011%2920%3A11%3C886%3A%3AAID-PD942%3E3.0.CO%3B2-4

10.1002%2Fjcb.23084

10.1159%2F000333060

Thông tin
Thông tin xuất bản

Fetal Diagnosis and Therapy

Tập 32 Số 3

194-200

Thông tin tác giả