A Biofloc-Based Aquaculture System Bio-augmented with Probiotic Bacteria Bacillus tequilensis AP BFT3 Improves Culture Environment, Production Performances, and Proteomic Changes in Penaeus vannamei
Tóm tắt
Experiments were conducted to evaluate the probiotic effect of bio-augmented Bacillus tequilensis AP BFT3 on improving production, immune response, and proteomic changes of Penaeus vannamei reared in a biofloc system. Penaeus vannamei larvae (PL13) were stocked in 100-L tanks at a rate of 100 no per tank to study the effect of B. tequilensis AP BFT3 with and without biofloc (BFT-PRO and PRO). Control tanks devoid of probiotic strain were maintained in a clear water system. The growth and survival considerably increased in probiotic added biofloc reared shrimp than probiotic added clear water reared ones and control. Water quality significantly improved in probiotic added (PRO) and biofloc-probiotics (BFT-PRO) system than control. Microbiological investigations indicate increased heterotrophic bacterial load in BFT-PRO compared to the PRO and control. The quality of the isolated microbes was analyzed in terms of enzyme production, and an abundance of enzyme-producing bacterial population was observed in BFT-PRO shrimp. Immune-related genes were significantly upregulated in BFT-PRO shrimp, followed by the PRO and control. The proteomic data (2D gel electrophoresis and MALDI-TOF) of muscle tissue from the experimental animals identified 11 differentially expressed proteins. The Daxx OS and Lit v 1 tropomyosin was found upregulated in BFT-PRO shrimps. Downregulation of Na+/K+ATPase was observed in biofloc with probiotic-supplied groups. The findings revealed that the BFT system’s efficacy could be improved through the addition of probiotics. The addition of B. tequilensis AP BFT3 as a probiotic in biofloc induced the expression of essential proteins, reducing contracting diseases during culture.
Tài liệu tham khảo
Emerenciano M, Ballester EL, Cavalli RO, Wasielesky W (2012) Biofloc technology application as a food source in a limited water exchange nursery system for pink shrimp Farfantepenaeus brasiliensis (Latreille, 1817). Aquac Res 43:447–457. https://doi.org/10.1111/j.1365-2109.2011.02848.x
Bondad-Reantaso MG, Subasinghe RP, Arthur JR, Ogawa K, Chinabut S, Adlard R, Tan Z, Sharif M (2005) Disease and health management in Asian aquaculture. Vet Parasitol 132:249–272. https://doi.org/10.1016/j.vetpar.2005.07.005
Crab R, Defoirdt T, Bossier P, Verstraete W (2012) Biofloc technology in aquaculture: beneficial effects and future challenges. Aquaculture 356–357:351–356. https://doi.org/10.1016/j.aquaculture.2012.04.046
Suantika G, Situmorang ML, Aditiawati P0, Astuti DI, Azizah FFN, Muhammad H (2017) Closed aquaculture system: zero water discharge for shrimp and prawn farming in Indonesia In book: Biological Resources of Water 70944. https://doi.org/10.5772/intechopen.70944
Emerenciano M, Martínez- Córdova L, Martínez-Porchas M, Miranda-Baeza A (2017) Biofloc technology (BFT): A tool for water quality management in aquaculture, water quality. Hlanganani Tutu, Intech Open. https://doi.org/10.5772/66416
Durigon EG, Lazzari R, Uczay J, de Alcântara Lopes DL, Jerônimo GT, Sgnaulina T, Emerenciano MGC (2020) Biofloc technology (BFT): adjusting the levels of digestible protein and digestible energy in diets of Nile tilapia juveniles raised in brackish water. Aquac Fish 5:42–51. https://doi.org/10.1016/j.aaf.2019.07.001
Panigrahi A, Sundaram M, Saranya C, Satishkumar R, Syama Dayal J, Gopal C (2019) Effect of carbon and nitrogen ratio (C: N) manipulation on the production performance and immunity of Pacific white shrimp Litopenaeus vannamei (Boone, 1931) in a biofloc-based rearing system. Aquac Res 50:29–41. https://doi.org/10.1111/are.13857
Panigrahi A, Sundaram M, Saranya C, Swain S, Dash RR, Syama Dayal J (2019) Carbohydrate sources deferentially influence growth performances, microbial dynamics and immunomodulation in Pacific white shrimp (Litopenaeus vannamei) under biofloc system. Fish Shellfish Immunol 86:1207–1216. https://doi.org/10.1016/j.fsi.2018.12.040
Avnimelech Y (1999) Carbon/nitrogen ratio as a control element in aquaculture systems. Aquaculture 176:227–235. https://doi.org/10.1016/S0044-84869900085-X
Emerenciano M, Gaxiola G, Cuzon G (2013) Biofloc technology (BFT): a review for aquaculture application and animal food industry. Biomass Now - Cultivation and Utilization. Miodrag Darko Matovi Intech Open. https://doi.org/10.5772/53902
Vargas-Albores F, Porchas-Cornejo MA, Martínez-Porchas M, Villalpando-Canchola E, Gollas-Galván T, Martínez-Córdova LR (2017) Bacterial biota of shrimp intestine is significantly modified by the use of a probiotic mixture: a high throughput sequencing approach. Helgol Mar Res 71:5. https://doi.org/10.1186/s10152-017-0485-z
Ferreira MG, Melo FP, Lima JP, Andrade HA, Severi W, Correia ES (2017) Bioremediation and biocontrol of commercial probiotic in marine shrimp culture with biofloc. Lat Am J Aquat Res 45:167–176. https://doi.org/10.3856/vol45-issue1-fulltext-16
Llario F, Falco S, Sebastiá-Frasquet MT, Escrivá J, Rodilla M, Poersch LH (2019) The role of Bacillus amyloliquefaciens on Litopenaeus vannamei during the maturation of a biofloc system. J Mar Sci Eng 7:228. https://doi.org/10.3390/jmse7070228
Wang YC, Hu SY, Chiu CS, Liu CH (2019) Multiple-strain probiotics appear to be more effective in improving the growth performance and health status of white shrimp, Litopenaeus vannamei, than single probiotic strains. Fish Shellfish Immunol 84:1050–1058. https://doi.org/10.1016/j.fsi.2018.11.017
Görg A, Weiss W, Dunn MJ (2004) Current two-dimensional electrophoresis technology for proteomics. Proteomics 4:3665–3685. https://doi.org/10.1002/pmic.200401031
Abbaszadeh A, Keyvanshokooh S, Yavari V, Naderi M (2019) Proteome modifications of Pacific white shrimp (Litopenaeus vannamei) muscle under biofloc system. Aquac Nut 25(2):358–366. https://doi.org/10.1111/anu.12861
Panigrahi A, Esakkiraj P, Jayashree S, Saranya C, Das RR, Sundaram M (2019) Colonization of enzymatic bacterial flora in biofloc grown shrimp Penaeus vannamei and evaluation of their beneficial effect. Aquac Int 27:1835–1846. https://doi.org/10.1007/s10499-019-00434-x
APHA (1998) (American Public Health Association), American water works association, water environment federation. Standard Methods for the Examination of Water and Wastewater, 20th ed. APHA, Washington, DC
Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT–PCR. Nucleic Acids Res 29:e45. https://doi.org/10.1093/nar/29.9.e45
Ananthi S, Prajna NV, Lalitha P, Valarnila M, Dharmalingam K (2013) Pathogen induced changes in the protein profile of human tears from Fusarium keratitis patients. PLoS One 8:e53018. https://doi.org/10.1371/journal.pone.0053018
Rajkumar M, Pandey PK, Aravind R, Vennila A, Bharti V, Purushothaman CS (2016) Effect of different biofloc system on water quality, biofloc composition and growth performance in Litopenaeus vannamei (Boone, 1931). Aquac Res 47:3432–3444. https://doi.org/10.1111/are.12792
Ponce-Palafox JT, Pavia ÁA, López DG, Arredondo-Figueroa JL, Lango-Reynoso F, Castañeda-Chávez MR, Esparza-Leal H, Ruiz-Luna A, Páez-Ozuna F, Castillo-Vargasmachuca SG, Peraza-Gómez V (2019) Response surface analysis of temperature-salinity interaction effects on water quality, growth and survival of shrimp Penaeus vannamei postlarvae raised in biofloc intensive nursery production. Aquaculture 503:312–321. https://doi.org/10.1016/j.aquaculture.2019.01.020
Prangnell DI, Castro LF, Ali AS, Browdy CL, Samocha TM (2020) The performance of juvenile Litopenaeus vannamei fed commercial diets of differing protein content, in a super-intensive biofloc-dominated system. J Appl Aquac 1–22. https://doi.org/10.1080/10454438.2020.1766632
Tong R, Chen W, Pan L, Zhang K (2020) Effects of feeding level and C/N ratio on water quality, growth performance, immune and antioxidant status of Litopenaeus vannamei in zero–water exchange bioflocs-based outdoor soil culture ponds. Fish Shellfish Immunol 101:126–134. https://doi.org/10.1016/j.fsi.2020.03.051
Avnimelech Y (2009) Biofloc technology: a practical guide book. World Aquaculture Society, Baton Rouge, United States
Crab R, Chielens B, Wille M, Bossier P, Verstraete W (2010) The effect of different carbon sources on the nutritional value of bioflocs, a feed for Macrobrachium rosenbergii postlarvae. Aquac Res 41:559–567. https://doi.org/10.1111/j.1365-2109.2009.02353.x
Zhao P, Huang J, Wang XH, Song XL, Yang CH, Zhang XG, Wang GC (2012) The application of bioflocs technology in high-intensive, zero exchange farming systems of Marsupenaeus japonicas. Aquaculture 354–355:97–106. https://doi.org/10.1016/j.aquaculture.2012.03.034
Xu WJ, Morris TC, Samocha TM (2016) Effects of C/N ratio on biofloc development, water quality, and performance of Litopenaeus vannamei juveniles in a biofloc-based, high-density, zero-exchange, outdoor tank system. Aquaculture 453:169–175. https://doi.org/10.1016/j.aquaculture.2015.11.021
Panigrahi A, Das RR, Sivakumar MR, Saravanan A, Saranya C, Sudheer NS, Kumaraguru Vasagam KP, Mahalakshmi P, Kannappan S, Gopikrishna G (2020) Bio-augmentation of heterotrophic bacteria in biofloc system improves growth, survival, and immunity of Indian white shrimp Penaeus indicus. Fish Shellfish Immunol 98:477–487. https://doi.org/10.1016/j.fsi.2020.01.021
Suita SM, Ballester ELC, Abreu PC, Wasielesky W Jr (2015) Dextrose as carbon source in the culture of Litopenaeus vannamei (Boone, 1931) in a zero-exchange system. Lat Am J Aquat Res 43:526–533. https://doi.org/10.3856/vol43-issue3-fulltext-13
Widarni J, Ekasari J, Maryam S (2012) Evaluation of biofloc technology application on water quality and production performance of Red Tilapia Oreochromis sp. cultured at different stocking densities. HAYATI J Biosci 19:73–80. https://doi.org/10.4308/hjb.19.2.73
Krummenauer D, Poersch L, Romano LA, Lara GR, Encarnação P, Wasielesky W Jr (2014) The effect of probiotics in a Litopenaeus vannamei biofloc culture system infected with Vibrio parahaemolyticus. J Appl Aquac 26:370–379. https://doi.org/10.1080/10454438.2014.965575
Gaona CAP, de Almeida MS, Viau V, Poersch LH, Wasielesky W Jr (2017) Effect of different total suspended solids levels on a Litopenaeus vannamei (Boone, 1931) BFT culture system during biofloc formation. Aquac Res 48:1070–1079. https://doi.org/10.1111/are.12949
Liu G, Zhu S, Liu D, Guo X, Ye Z (2017) Effects of stocking density of the white shrimp Litopenaeus vannamei (Boone) on immunities, antioxidant status, and resistance against Vibrio harveyi in a biofloc system. Fish Shellfish Immunol 67:19–26. https://doi.org/10.1016/j.fsi.2017.05.038
Bentzon-Tilia M, Sonnenschein EC, Gram L (2016) Monitoring and managing microbes in aquaculture–towards a sustainable industry. Microbial Biotechnol 9:576–584. https://doi.org/10.1111/1751-7915.12392
Esakkiraj P, Dhas A, Palavesam A, Immanuel G (2010) Media preparation using tuna-processing wastes for improved lipase production by shrimp gut isolate Staphylococcus epidermidis CMST Pi 2. Appl Biochem Biotechnol 160:1254–1265. https://doi.org/10.1007/s12010-009-8632-x
Banerjee S, Ghosh K (2014) Enumeration of gut associated extracellular enzyme-producing yeasts in some freshwater fishes. J Appl Ichthyol 30:986–993. https://doi.org/10.1111/jai.12457
Liu H, Guo X, Gooneratne R, Lai R, Zeng C, Zhan F, Wang W (2016) The gut microbiome and degradation enzyme activity of wild freshwater fishes influenced by their trophic levels. Sci Rep 6:24340. https://doi.org/10.1038/srep24340
Ekasari J, Azhar MH, Surawidjaja EH, Nuryati S, De Schryver P, Bossier P (2014) Immune response and disease resistance of shrimp fed biofloc grown on different carbon sources. Fish Shellfish Immunol 41:332–339. https://doi.org/10.1016/j.fsi.2014.09.004
Ferreira GS, Bolívar NC, Pereira SA, Guertler C, do Nascimento Vieira F, Mouriño JLP, Seiffert WQ, (2015) Microbial biofloc as source of probiotic bacteria for the culture of Litopenaeus vannamei. Aquaculture 448:273–279. https://doi.org/10.1016/j.aquaculture.2015.06.006
Schleder DD, Jatobá A, Silva BC, Ferro DP, Seiffert WQ, Vieira FN (2018) Soybean protein concentrate in Pacific white shrimp reared in bioflocs: effect on health and Vibrio challenge. Acta Sci 40:e42570. https://doi.org/10.4025/actascianimsci.v40i1.42570
Aguilera Rivera D, Escalante Herrera K, Gaxiola G, Prieto Davó A, Fuentes GR, Castro EG, Hernández-López J, Chávez-Sánchez MC, Rodríguez-Canul R (2019) Immune response of the Pacific white shrimp, Litopenaeus vannamei, previously reared in biofloc and after an infection assay with Vibrio harveyi. J World Aquac Soc 50:119–136. https://doi.org/10.1111/jwas.12543
Panigrahi A, Saranya C, Sundaram M, Vinoth Kannan SR, Das RR, Satish Kumar R, Rajesh P, Otta SK (2018) Carbon: Nitrogen (C: N) ratio level variation influences microbial community of the system and growth as well as immunity of shrimp (Litopenaeus vannamei) in biofloc based culture system. Fish Shellfish Immunol 81:329–337. https://doi.org/10.1016/j.fsi.2018.07.035
Kim MS, Min E, Kim JH, Koo JK, Kang JC (2015) Growth performance and immunological and antioxidant status of Chinese shrimp, Fennerpenaeus chinensis reared in bio-floc culture system using probiotics. Fish Shellfish Immunol 47:141–146. https://doi.org/10.1016/j.fsi.2015.08.027
De Souza DM, Suita SM, Leite FP, Romano LA, Wasielesky W, Ballester ELC (2012) The use of probiotics during the nursery rearing of the pink shrimp Farfantepenaeus brasiliensis (Latreille, 1817) in a zero exchange system. Aquac Res 43:1828–1837. https://doi.org/10.1111/j.1365-2109.2011.02992.x
Miao S, Zhu J, Zhao C, Sun L, Zhang X, Chen G (2017) Effects of C/N ratio control combined with probiotics on the immune response, disease resistance, intestinal microbiota and morphology of giant freshwater prawn (Macrobrachium rosenbergii). Aquaculture 476:125–133. https://doi.org/10.1016/j.aquaculture.2017.04.027
Pattukumar V, Kanmani P, Satish Kumar R, Yuvaraj N, Paari A, Arul V (2014) Enhancement of innate immune system, survival and yield in Penaeus monodon reared in ponds using Streptococcus phocae PI 80. Aquac Nut 20:505–513. https://doi.org/10.1111/anu.12103
Sharifuzzaman SM, Austin B (2017) Probiotics for disease control in aquaculture In Diagnosis and Control of Diseases of Fish and Shellfish 189–222. https://doi.org/10.1002/9781119152125.ch8
Rengpipat S, Rukpratanporn S, Piyatiratitivorakul S, Menasaveta P (2000) Immunity enhancement in black tiger shrimp (Penaeus monodon) by a probiont bacterium (Bacillus S11). Aquaculture 191:271–288. https://doi.org/10.1016/S0044-848600440-3
Verschuere L, Rombaut G, Huys G, Dhont J, Sorgeloos P, Verstraete V (1999) Microbial control of the culture of Artemia juveniles through preemptive colonization by selected bacterial strains. Appl Environ Microbiol 65:2527–2533. https://doi.org/10.1128/AEM.65.6.2527-2533.1999
Balcázar JL, De Blas I, Ruiz-Zarzuela I, Cunningham D, Vendrell DL, Muzquiz J (2006) The role of probiotics in aquaculture. Vet Microbiol 114:173–186. https://doi.org/10.1016/S0044-8486(00)00440-3
Wang PH, Gu ZH, Wan DH, Liu BD, Huang XD, Weng SP, Yu XG, He JG (2013) The shrimp IKK-NF-κB signaling pathway regulates antimicrobial peptide expression and may be subverted by White spot syndrome virus to facilitate viral gene expression. Cell Mol Immunol 10:423–36. https://doi.org/10.1038/cmi.2013.30
Wang YB (2007) Effect of probiotics on growth performance and digestive enzyme activity of the shrimp Penaeus vannamei. Aquaculture 269:259–264. https://doi.org/10.1016/j.aquaculture.2007.05.035
Shen WY, Fu LL, Li WF, Zhu YR (2010) Effect of dietary supplementation with Bacillus subtilis on the growth, performance, immune response and antioxidant activities of the shrimp (Litopenaeus vannamei). Aquac Res 41:1691–1698. https://doi.org/10.1111/j.1365-2109.2010.02554.x
Yan M, Tang J, Liang Q, Zhu G, Li H, Li C, Weng S, He J, Xu X (2015) Daxx from Pacific white shrimp Litopenaeus vannamei is involved in activation of NF-kB pathway. Fish Shellfish Immunol 45:443–453. https://doi.org/10.1016/j.fsi.2015.04.027
Chongsatja PO, Bourchookarn A, Lo CF, Thongboonkerd V, Krittanai C (2007) Proteomic analysis of differentially expressed proteins in Penaeus vannamei hemocytes upon Taura syndrome virus infection. Proteomics 7:3592–3601. https://doi.org/10.1002/pmic.200700281
Kulkarni AD, Kiron V, Rombout JH, Brinchmann MF, Fernandes JM, Sudheer NS, Bright Singh IS (2014) Protein profiling in the gut of Penaeus monodon gavaged with oral WSSV-vaccines and live white spot syndrome virus. Proteomics 14:1660–1673. https://doi.org/10.1002/pmic.201300405
Gunning PW, Hardeman EC (2017) Tropomyosins. Curr Biol 27:R8–R13. https://doi.org/10.1016/j.cub.2016.11.033
Gunning PW, Hardeman EC, Lappalainen P, Mulvihill DP (2015) Tropomyosin – master regulator of actin filament function in the cytoskeleton. J Cell Sci 128:2965–2974. https://doi.org/10.1242/jcs.172502
Palacios E, Bonilla A, Luna D, Racotta IS (2004) Survival, Na+/K+-ATPase and lipid responses to salinity challenge in fed and starved white pacific shrimp (Litopenaeus vannamei) post larvae. Aquaculture 234:497–511. https://doi.org/10.1016/j.aquaculture.2003.12.001
Wang L, Wang WN, Liu Y, Cai DX, Li JZ, Wang AN (2012) Two types of ATPases from the Pacific white shrimp, Litopenaeus vannamei in response to environmental stress. Mol Biol Rep 39:6427–6438. https://doi.org/10.1007/s11033-012-1461-y
Kuebutornye FKA, Abarike ED, Lu Y, Hlordzi V, Sakyi ME, Afriyie G, Wang Z, Li Y, Xie CX (2020) Mechanisms and the role of probiotic Bacillus in mitigating fish pathogens in aquaculture. Fish Physiol Biochem 46:819–841. https://doi.org/10.1007/s10695-019-00754-y
Gao XY, Liu Y, Miao LL, Li EW, Hou TT, Liu ZP (2017) Mechanism of anti-Vibrio activity of marine probiotic strain Bacillus pumilus H2, and characterization of the active substance. AMB Expr 7:23. https://doi.org/10.1186/s13568-017-0323-3
Maksimova EM, Vinogradova DS, Osterman IA, Kasatsky PS, Nikonov OS, Milón P, Dontsova OA, Sergiev PV, Paleskava A, Konevega AL (2021) Multifaceted mechanism of amicoumacin A: inhibition of Bacterial Translation. Front Microbiol 12:618857. https://doi.org/10.3389/fmicb.2021.618857
Parveen Rani R, Anandharaj M, Hema S, Deepika R, David Ravindran A (2016) Purification of antilisterial peptide (subtilosin A) from novel Bacillus tequilensis FR9 and demonstrate their pathogen invasion protection ability using human carcinoma cell line. Front Microbiol 7:1910. https://doi.org/10.3389/fmicb.2016.01910