Association of Protein Kinase A and Protein Phosphatase 2B with a Common Anchoring Protein

American Association for the Advancement of Science (AAAS) - Tập 267 Số 5194 - Trang 108-111 - 1995
Vincent M. Coghlan1, Brian A. Perrino1, Monique Howard2, Lorene K. Langeberg1, James Hicks2, W. Michael Gallatin2, John D. Scott1
1Vollum Institute, Oregon Health Sciences University, Portland, OR 97201
2ICOS Corporation, Bothell, WA 98021, USA

Tóm tắt

Specificity of protein kinases and phosphatases may be achieved through compartmentalization with preferred substrates. In neurons, adenosine 3′,5′-monophosphate (cAMP)-dependent protein kinase (PKA) is localized at postsynaptic densities by association of its regulatory subunit with an A kinase anchor protein, AKAP79. Interaction cloning experiments demonstrated that AKAP79 also binds protein phosphatase 2B, or calcineurin (CaN). A ternary complex of PKA, AKAP, and CaN was isolated from bovine brain, and colocalization of the kinase and the phosphatase was established in neurites of cultured hippocampal neurons. The putative CaN-binding domain of AKAP79 is similar to that of the immunophilin FKBP-12, and AKAP79 inhibited CaN phosphatase activity. These results suggest that both PKA and CaN are targeted to subcellular sites by association with a common anchor protein and thereby regulate the phosphorylation state of key neuronal substrates.

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Tài liệu tham khảo

ALDAPE, R.A., CHARGED SURFACE RESIDUES OF FKBP12 PARTICIPATE IN FORMATION OF THE FKBP12-FK506-CALCINEURIN COMPLEX, JOURNAL OF BIOLOGICAL CHEMISTRY 267: 16029 (1992).

BRAUTIGAN, D. L., RECENT PROGRESS IN HORMONE RESEARCH, VOL 49 49: 197 (1994).

BREGMAN, D.B., HIGH-AFFINITY BINDING-PROTEIN FOR THE REGULATORY SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE-II-B - CLONING, CHARACTERIZATION, AND EXPRESSION OF CDNAS FOR RAT-BRAIN P150, JOURNAL OF BIOLOGICAL CHEMISTRY 264: 4648 (1989).

BREGMAN, D.B., MOLECULAR CHARACTERIZATION OF BOVINE BRAIN-P75, A HIGH-AFFINITY BINDING-PROTEIN FOR THE REGULATORY SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE-II-BETA, JOURNAL OF BIOLOGICAL CHEMISTRY 266: 7207 (1991).

CARR, D.W., INTERACTION OF THE REGULATORY SUBUNIT (RII) OF CAMP-DEPENDENT PROTEIN-KINASE WITH RII-ANCHORING PROTEINS OCCURS THROUGH AN AMPHIPATHIC HELIX BINDING MOTIF, JOURNAL OF BIOLOGICAL CHEMISTRY 266: 14188 (1991).

CARR, D.W., LOCALIZATION OF THE CAMP-DEPENDENT PROTEIN-KINASE TO THE POSTSYNAPTIC DENSITIES BY A-KINASE ANCHORING PROTEINS - CHARACTERIZATION OF AKAP-79, JOURNAL OF BIOLOGICAL CHEMISTRY 267: 16816 (1992).

CARR, D.W., ASSOCIATION OF THE TYPE-II CAMP-DEPENDENT PROTEIN-KINASE WITH A HUMAN THYROID RII-ANCHORING PROTEIN - CLONING AND CHARACTERIZATION OF THE RII-BINDING DOMAIN, JOURNAL OF BIOLOGICAL CHEMISTRY 267: 13376 (1992).

CHIEN, C.T., THE 2-HYBRID SYSTEM - A METHOD TO IDENTIFY AND CLONE GENES FOR PROTEINS THAT INTERACT WITH A PROTEIN OF INTEREST, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 9578 (1991).

COGHLAN, V.M., CLONING AND CHARACTERIZATION OF AKAP-95, A NUCLEAR-PROTEIN THAT ASSOCIATES WITH THE REGULATORY SUBUNIT OF TYPE-II CAMP-DEPENDENT PROTEIN-KINASE, JOURNAL OF BIOLOGICAL CHEMISTRY 269: 7658 (1994).

COGHLAN, V.M., A-KINASE ANCHORING PROTEINS - A KEY TO SELECTIVE ACTIVATION OF CAMP-RESPONSIVE EVENTS, MOLECULAR AND CELLULAR BIOCHEMISTRY 128: 309 (1993).

COGHLAN V.M. THESIS.

COHEN, P, THE STRUCTURE AND REGULATION OF PROTEIN PHOSPHATASES, ANNUAL REVIEW OF BIOCHEMISTRY 58: 453 (1989).

DURFEE, T, THE RETINOBLASTOMA PROTEIN ASSOCIATES WITH THE PROTEIN PHOSPHATASE TYPE-1 CATALYTIC SUBUNIT, GENES & DEVELOPMENT 7: 555 (1993).

GRAB, D.J., PRESENCE OF CALMODULIN IN POSTSYNAPTIC DENSITIES ISOLATED FROM CANINE CEREBRAL-CORTEX, JOURNAL OF BIOLOGICAL CHEMISTRY 254: 8690 (1979).

Harlow, E., Antibodies: A Laboratory Manual: 421 (1988).

HATHAWAY, D.R., INTERACTION OF CALMODULIN WITH MYOSIN LIGHT CHAIN KINASE AND CAMP-DEPENDENT PROTEIN-KINASE IN BOVINE BRAIN, JOURNAL OF BIOLOGICAL CHEMISTRY 256: 8183 (1981).

HEMMINGS, H.C., ROLE OF PROTEIN-PHOSPHORYLATION IN NEURONAL SIGNAL TRANSDUCTION, FASEB JOURNAL 3: 1583 (1989).

HIRSCH, A.H., CLONING AND EXPRESSION OF AN INTRON-LESS GENE FOR AKAP-75, AN ANCHOR PROTEIN FOR THE REGULATORY SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE-II-BETA, JOURNAL OF BIOLOGICAL CHEMISTRY 267: 2131 (1992).

HUBBARD, M.J., ON TARGET WITH A NEW MECHANISM FOR THE REGULATION OF PROTEIN-PHOSPHORYLATION, TRENDS IN BIOCHEMICAL SCIENCES 18: 172 (1993).

KLEE, C.B., CALCINEURIN, ADVANCES IN ENZYMOLOGY AND RELATED AREAS OF MOLECULAR BIOLOGY 61: 149 (1988).

KOFRON, J.L., DETERMINATION OF KINETIC CONSTANTS FOR PEPTIDYL PROLYL CIS TRANS ISOMERASES BY AN IMPROVED SPECTROPHOTOMETRIC ASSAY, BIOCHEMISTRY 30: 6127 (1991).

KREBS, E.G., PHOSPHORYLATION-DEPHOSPHORYLATION OF ENZYMES, ANNUAL REVIEW OF BIOCHEMISTRY 48: 923 (1979).

KUNO, T, EVIDENCE FOR A 2ND ISOFORM OF THE CATALYTIC SUBUNIT OF CALMODULIN-DEPENDENT PROTEIN PHOSPHATASE (CALCINEURIN-A), BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 165: 1352 (1989).

LIU, J, CALCINEURIN IS A COMMON TARGET OF CYCLOPHILIN-CYCLOSPORINE-A AND FKBP-FK506 COMPLEXES, CELL 66: 807 (1991).

LOHMANN, S.M., HIGH-AFFINITY BINDING OF THE REGULATORY SUBUNIT (RII) OF CAMP-DEPENDENT PROTEIN-KINASE TO MICROTUBULE-ASSOCIATED AND OTHER CELLULAR PROTEINS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 81: 6723 (1984).

LUDVIG, N, IMMUNOCYTOCHEMICAL LOCALIZATION OF THE NEURAL-SPECIFIC REGULATORY SUBUNIT OF THE TYPE-II CYCLIC AMP-DEPENDENT PROTEIN-KINASE TO POSTSYNAPTIC STRUCTURES IN THE RAT-BRAIN, BRAIN RESEARCH 520: 90 (1990).

PERRINO, B.A., CHARACTERIZATION OF THE PHOSPHATASE-ACTIVITY OF A BACULOVIRUS-EXPRESSED CALCINEURIN-A ISOFORM, JOURNAL OF BIOLOGICAL CHEMISTRY 267: 15965 (1992).

ROSENMUND, C, ANCHORING OF PROTEIN-KINASE-A IS REQUIRED FOR MODULATION OF AMPA/KAINATE RECEPTORS ON HIPPOCAMPAL-NEURONS, NATURE 368: 853 (1994).

SCOTT, J.D., LOCALIZATION OF A-KINASE THROUGH ANCHORING PROTEINS, MOLECULAR ENDOCRINOLOGY 8: 5 (1994).

SCOTT, J.D., IDENTIFICATION OF AN INHIBITORY REGION OF THE HEAT-STABLE PROTEIN INHIBITOR OF THE CAMP-DEPENDENT PROTEIN-KINASE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 82: 4379 (1985).

STEMMER, P, CURR OPINION NEUROBI 1: 53 (1991).

WOOD, J.G., IMMUNOCYTOCHEMICAL LOCALIZATION OF CALMODULIN AND A HEAT-LABILE CALMODULIN-BINDING PROTEIN (CAM-BP80) IN BASAL GANGLIA OF MOUSE-BRAIN, JOURNAL OF CELL BIOLOGY 84: 66 (1980).

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American Association for the Advancement of Science (AAAS)

Tập 267 Số 5194

108-111

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