Fe nutrition demand and utilization by the green alga Dunaliella bardawil
Tóm tắt
The Fe nutritional demands, requirements and mechanisms of uptake by Dunaliella bardawil as well as potential Fe sources were studied. A comparison between Fe uptake from bacterial siderophores and from synthetic ferric chelates revealed algal growth response and chlorophyll synthesis to increasing concentrations and availability at a range of 0.01 μM–5 μM, as well as differences in efficiency. Furthermore, chloroplast ultrastructure, as observed by TEM, was affected by Fe deficiency, as was chlorophyll content. Ferric reduction is involved in the Fe uptake mechanism of Fe-stressed D. bardawil. Nutrient solution with controlled levels of free Fe2+ as well as spectrophotometric assays were used to measure Fe3+ reduction. This study shows that D. bardawil utilizes Fe3+ via a reduction mechanism, similar to that of strategy-I higher plants.
Tài liệu tham khảo
Allnutt F C and Bonner W D 1987a Evaluation of reductive release as a mechanism for Fe uptake from ferrioamine B by Chlorella vulgaris Plant Physiol. 85, 746-750.
Allnutt F C and Bonner W D 1987b Characterization of Fe uptake from ferrioxamine B by Chlorella vulgaris. Plant Physiol. 85, 751-756.
Anderson M A and Morel F M 1980 Uptake of Fe(II) by a diatom in oxic culture medium. Mar. Biol. Lett. 1, 263-268.
Arnon D I 1949 Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24, 1-15.
Bar-Ness E, Hadar Y, Chen Y, Römheld V and Marschner H 1992 Short-term effects of rhizosphere microorganisms on Fe uptake from microbial siderophores bymaize and oat. Plant Physiol 100, 451-456.
Benderliev K M and Ivanova N I 1994 High affinity siderophore mediated iron transport system in the green alga Scenedesmus incrassatulus. Planta 193, 163-166.
Ben-Amotz A and Avron M 1990 The biotechnology of cultivating the halotolerantalga Dunaliella. TIB. 8, 121-126.
Bienfait H F, Bin, Van Der Bliek A M and Fontaine J M L 1983 Characterization of ferric reducing activity in roots of Fe-deficient Phaseolus vulgaris. Physiol. Plant 59, 196-202.
Chen Y, Chaney R L and Bell P 1991 Determination of Fe2C requirements of plant using ferrozine-buffered nutrient solution. In Proc. Int. Iron Symp. 6th. Logan, UT. 14-19 July 1991. pp 40. Utah State Univ., Logan. UT.
Crowley D E, Reid C P P NS Szaniszlo P J 1991 Mechanisms of iron acquisition from siderophores by microorganisms and plants. In Eds Y Chen and Y Hadar. Iron Nutrition and Interactions in Plants. pp. 213-232. Kluwer Academic Publishers, Dordrecht, The Netherlands.
Fox T C, Shaff E S, Grusak M A, Norvell W A, Chen Y, Chaney R L and Kochian L V 1996 Direct measurement of 59Fe-labeled Fe2C influx in roots of Pea using a chelator buffer system to control free Fe2C in solution. Plant Physiol. 111, 93-100.
Guerinot ML and Yi Y 1994 Fe: nutritious, noxious, and not readily available. Plant Physiol. 104, 815-820.
Jurkevitch E, Hadar Y and Chen Y 1988 Involvement of bacterial siderophores in the remedy of lime-induced chlorosis in peanut. Soil Sci. Soc. Am. J. 52, 1032-1037.
Keshtacher-Leibson E, Hadar Y and Chen Y 1995 Oligotrophic bacteria enhance algal growth under Fe deficient conditions. Appl. Envir. Microbiol. 61, 2439-2441.
Kochian L V 1991 Mechanisms of micronutrient uptake, translocation, and interactions in plants. In Micronutrients in Agriculture. 2nd ed. Eds JJ Mortvedt, FR Cox, LM Shuman and RM Welch. pp. 229-296. Soil Science Society of America, Madison, WI.
Marschner H and Römheld V 1994 Strategies of plants for acquisition of Fe. Plant Soil 165, 261-274.
Martin J H, Fitzwater S E 1988 Iron deficiency limits phytoplankton growth in north-east Pacific subarctic. Nature 331, 341-343.
Martin J H, Gordon R M and Fitzwater S E 1990 Iron in antarctic waters. Nature 345, 156-158.
Moog P R and Bruggemann W 1994 Iron reductase system on the plant plasma membrane-a review. Plant Soil 165, 241-260.
Murphy T P and Lean D R 1976 Blue-green algae: Their excretion of Fe-selective chelators enables them to dominate other algae. Science 192, 900-903.
Neilands J B 1990 Siderophore system of bacteria and fungi Adv. Inorg. Biochem. 8, 63-90.
Parker D R, Norvell W A and Chaney R L 1995 GEOCHEM-PC: A chemical speciation program for IBM and compatible personal computers. In Soil Chemical Equilibrium and Reaction Models. Eds RH Loeppert, AP Schwab and S Goldberg. SSSA Spec. Pub. No 42, ASA, SSSA, Madison, WI.
Platt-Aloia K A, Thomson W W, Terry N 1983 Changes in plasmid ultrastructure during Fe nutrition mediated chloroplast development. Protoplasma 114, 85-92.
Reid R T, Live D H, Faulkner D J and Butler A 1993 A siderophore from a marine bacterium with an exceptional ferric ion affinity constant. Nature 366, 455-458.
Römheld V 1987 Existence of two different strategies for the acquisition of Fe in higher plants. In Fe Transport in Microbes, Plants and Animals. Eds D Van der Helm, JB Neilands and G Winkelmann. pp 353-374. Verlag Chemie, Weinheim, FRG.
Sposito G and Mattigod S V 1980 GEOCHEM: A computer program for the calculation of chemical equilibria in soil solutions and other natural water systems. Kearney Foundation of soil science, Univ. of California, Riverside.
Terry N and Abadia J 1986 Function of Fe in chloroplasts. J. Plant Nutr. 9, 609-646.
Thiel G, Kirst G O 1988 Transmembrane ferricyanide reduction and membrane properties in the euryhaline charophyte Lamprothaminium papulosum. J. Exp. Bot. 39, 641-654.
Welch R M 1995 Micronutrient nutrition of plants. Critical Reviews in Plant Sciences 14, 49-82.