Stable, Monomeric Variants of λ Cro Obtained by Insertion of a Designed β-Hairpin Sequence

American Association for the Advancement of Science (AAAS) - Tập 250 Số 4988 - Trang 1712-1715 - 1990
Michael C. Mossing1, Robert T. Sauer1
1Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139;

Tóm tắt

λ Cro is a dimeric DNA binding protein. Random mutagenesis and a selection for Cro activity have been used to identify the contacts between Cro subunits that are crucial for maintenance of a stably folded structure. To obtain equivalent contacts in a monomeric system, a Cro variant was designed and constructed in which the antiparallel β-ribbon that forms the dimer interface was replaced by a β-hairpin. The engineered monomer has a folded structure similar to wild type, is significantly more stable than wild type, and exhibits novel half-operator binding activity.

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Tài liệu tham khảo

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American Association for the Advancement of Science (AAAS)

Tập 250 Số 4988

1712-1715

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