A quantitative method for the analysis of mammalian cell proliferation in culture in terms of dividing and non‐dividing cells

Cell Proliferation - Tập 28 Số 3 - Trang 137-144 - 1995
James L. Sherley1, Patrizia B. Stadler1, J.S. Stadler2
1Department of Molecular Oncology, Division of Medical Science, Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA
2Department of Electrical Engineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA

Tóm tắt

AbstractThe application of the exponential growth equation is the standard method employed in the quantitative analyses of mammalian cell proliferation in culture. This method is based on the implicit assumption that, within a cell population under study, all division events give rise to daughter cells that always divide. When a cell population does not adhere to this assumption, use of the exponential growth equation leads to errors in the determination of both population doubling time and cell generation time. We have derived a more general growth equation that defines cell growth in terms of the dividing fraction of daughter cells. This equation can account for population growth kinetics that derive from the generation of both dividing and non‐dividing cells. As such, it provides a sensitive method for detecting non‐exponential division dynamics. In addition, this equation can be used to determine when it is appropriate to use the standard exponential growth equation for the estimation of doubling time and generation time.

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Cell Proliferation

Tập 28 Số 3

137-144

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