A Comprehensive Survey of the Plasmodium Life Cycle by Genomic, Transcriptomic, and Proteomic Analyses

American Association for the Advancement of Science (AAAS) - Tập 307 Số 5706 - Trang 82-86 - 2005
Neil Hall1,2,3,4,5, Marianna Karras1,2,3,4,5, J. Dale Raine1,2,3,4,5, Jane M. Carlton1,2,3,4,5, Taco W. A. Kooij1,2,3,4,5, Matthew Berriman1,2,3,4,5, Laurence Florens1,2,3,4,5, Christoph S. Janssen1,2,3,4,5, Arnab Pain1,2,3,4,5, George K. Christophides1,2,3,4,5, Keith James1,2,3,4,5, Kim Rutherford1,2,3,4,5, Barbara Harris1,2,3,4,5, D. James Harris1,2,3,4,5, Carol Churcher1,2,3,4,5, Michael A. Quail1,2,3,4,5, Doug Ormond1,2,3,4,5, Jon Doggett1,2,3,4,5, Holly E. Trueman1,2,3,4,5, Jacqui Mendoza1,2,3,4,5, Shelby Bidwell1,2,3,4,5, Alasdair Ivens1,2,3,4,5, Daniel J. Carucci1,2,3,4,5, John R. Yates1,2,3,4,5, Fotis C. Kafatos1,2,3,4,5, Chris J. Janse1,2,3,4,5, Bart Barrell1,2,3,4,5, C. Michael R. Turner1,2,3,4,5, Andrew P. Waters1,2,3,4,5, Robert E. Sinden1,2,3,4,5
1Department of Parasitology, Malaria Group, Leiden University Medical Center, Netherlands.
2Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32608, USA
3Immunology and Infection Section, Department of Biological Sciences, Imperial College London, Sir Alexander Fleming Building, Imperial College Road, London SW7 2AZ, UK.
4Pathogen Sequencing Unit, The Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridge CB10 1SA, UK.
5The Institute for Genomic Research (TIGR), 9712 Medical Center Drive, Rockville, MD 20850, USA

Tóm tắt

Plasmodium berghei and Plasmodium chabaudi are widely used model malaria species. Comparison of their genomes, integrated with proteomic and microarray data, with the genomes of Plasmodium falciparum and Plasmodium yoelii revealed a conserved core of 4500 Plasmodium genes in the central regions of the 14 chromosomes and highlighted genes evolving rapidly because of stage-specific selective pressures. Four strategies for gene expression are apparent during the parasites' life cycle: (i) housekeeping; (ii) host-related; (iii) strategy-specific related to invasion, asexual replication, and sexual development; and (iv) stage-specific. We observed posttranscriptional gene silencing through translational repression of messenger RNA during sexual development, and a 47-base 3′ untranslated region motif is implicated in this process.

Từ khóa


Tài liệu tham khảo

R. E. Sinden, in Rodent Malaria, R. Killick-Kendrick, W. Peters, Eds. (Academic Press, London, 1978), pp. 85–168.

10.1038/nature01097

10.1038/nature01099

Materials and methods are available as supporting material on Science Online.

10.1093/nar/30.7.1575

10.1101/gr.2126104

10.1038/35071118

10.1046/j.1365-2958.2003.03491.x

10.1098/rspb.2001.1903

10.1371/journal.pbio.0000045

10.1093/oxfordjournals.molbev.a025629

10.1038/nature01262

10.1093/emboj/19.22.6030

10.1038/85686

10.1038/208762a0

10.1051/parasite/2000071019

10.1016/j.molbiopara.2003.08.009

10.1038/nature02458

10.1038/nm785

10.1126/science.1087025

10.1038/nature01107

10.1371/journal.pbio.0000005

10.1126/science.1089799

10.1016/0166-6851(93)90224-L

10.1093/nar/gkf600

10.1046/j.1365-2958.2000.01730.x

10.1089/cmb.1998.5.211

10.1101/gr.2218604

10.1101/gr.849004

We thank J. Dame (University of Florida) for the gift of the P. berghei GSS library J. Langhorne (National Institute of Medical Research) for providing P. c. chabaudi DNA R. G. Sadygov (The Scripps Research Institute) for expert computer programming G. Butcher (Imperial College London) and M. Gardner (TIGR) for helpful advice with this manuscript and M. Aslett and T. Kulikova for assisting with the database submission. Supported by the Wellcome Trust the European Union the Office of Naval Research the U.S. Army Medical Research and Materiel Command and the NIH. J.D.R. and J.M. are funded by the Wellcome Trust M.K. by EU grant nos. RTN1-1999-00008 and QLK2-CT-1999-00753 and grant no. 050-10-053 from the Nederlands organisatie voor Wetenschappelijk Onderzoek and H.E.T. by the EU MALTRANS consortium. The sequences have been submitted to EMBL under the accession prefixes CAAI for P. berghei and CAAJ for P. chabaudi . All data sets are available through the Plasmodium genome sequence data repository PlasmoDB at www.plasmodb.org and genome annotation at www.genedb.org.

Thông tin
Thông tin xuất bản

American Association for the Advancement of Science (AAAS)

Tập 307 Số 5706

82-86

Thông tin tác giả