Junying Yu1,2,3, Kejin Hu3, Kim Smuga-Otto1,2,3, Shulan Tian1,2, Ron Stewart1,2, Igor I. Slukvin4,3, James A. Thomson5,1,2,3
1Genome Center of Wisconsin, Madison, WI 53706–1580, USA.
2Morgridge Institute for Research, Madison, WI 53707–7365, USA.
3Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI 53715–1299, USA.
4Department of Pathology and Laboratory Medicine, University of Wisconsin—Madison, Madison, WI, 53706, USA
5Department of Anatomy, University of Wisconsin-Madison, Madison, WI 53706–1509, USA.
Tóm tắt
Designer Stem Cells
Despite their promise for use as disease models and in regenerative medicine, the generation of human-induced pluripotent stem (iPS) cells has been hindered by the integration of vector and transgenes in the host cell genome. Recent studies using the Cre/LoxP recombination strategy and the piggyBac transposon approach have approached this objective. However,
Yu
et al.
(p.
797
, published online 26 March) now show the derivation of human iPS cells from postnatal foreskin fibroblasts using the nonintegrating oriP/EBNA1-based episomal vectors. The resultant iPS cells show characteristics of human embryonic stem cells and are free of vector and transgenes.
