Fura‐2 diffusion and its use as an indicator of transient free calcium changes in single striated muscle cells

FEBS Letters - Tập 209 Số 1 - Trang 1-8 - 1986
M. Timmerman1, Christopher Ashley1
1University Laboratory of Physiology, Parks Road, Oxford OX1 3PT, England

Tóm tắt

Two questions bearing on the use of fura‐2 to measure transient changes in intracellular Ca2+ concentration have been addressed. To investigate fura‐2 intracellular binding, the amounts of fura‐2 and [14C]glycine in Balanus nubilus myofibrillar bundles after loading were determined and their intracellular apparent diffusion constants measured. No significant fura‐2 immobilisation occurs under the conditions used. The apparent diffusion constant for fura‐2 in aqueous solution was determined. The relationship between half‐time for relaxation of force and fura‐2 fluorescence transients, and intracellular fura‐2 concentration, in voltageclamped single muscle fibres was examined. Significant buffering of the Ca2+ transient occurred at fura‐2 concentrations above ~ 6 μM.

Từ khóa


Tài liệu tham khảo

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FEBS Letters

Tập 209 Số 1

1-8

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