QSulf1 remodels the 6-O sulfation states of cell surface heparan sulfate proteoglycans to promote Wnt signaling

Journal of Cell Biology - Tập 162 Số 2 - Trang 341-351 - 2003
Xingbin Ai1, Anh-Tri Do2, Olga Lozynska3, Marion Kusche‐Gullberg2, Ulf Lindahl2, Charles P. Emerson3
1Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 USA
22Department of Medical Biochemistry and Microbiology, Uppsala University Biomedical Center, S-75123 Uppsala, Sweden
31Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104

Tóm tắt

The 6-O sulfation states of cell surface heparan sulfate proteoglycans (HSPGs) are dynamically regulated to control the growth and specification of embryonic progenitor lineages. However, mechanisms for regulation of HSPG sulfation have been unknown. Here, we report on the biochemical and Wnt signaling activities of QSulf1, a novel cell surface sulfatase. Biochemical studies establish that QSulf1 is a heparan sulfate (HS) 6-O endosulfatase with preference, in particular, toward trisulfated IdoA2S-GlcNS6S disaccharide units within HS chains. In cells, QSulf1 can function cell autonomously to remodel the sulfation of cell surface HS and promote Wnt signaling when localized either on the cell surface or in the Golgi apparatus. QSulf1 6-O desulfation reduces XWnt binding to heparin and HS chains of Glypican1, whereas heparin binds with high affinity to XWnt8 and inhibits Wnt signaling. CHO cells mutant for HS biosynthesis are defective in Wnt-dependent Frizzled receptor activation, establishing that HS is required for Frizzled receptor function. Together, these findings suggest a two-state “catch or present” model for QSulf1 regulation of Wnt signaling in which QSulf1 removes 6-O sulfates from HS chains to promote the formation of low affinity HS–Wnt complexes that can functionally interact with Frizzled receptors to initiate Wnt signal transduction.

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Journal of Cell Biology

Tập 162 Số 2

341-351

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