Cooperative Regulation of Cell Polarity and Growth by Drosophila Tumor Suppressors
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. Alleles used to generate clones were scrib 1− FRT82B scrib 2− FRT82B 4w3-FRT40 lgl 4− FRT40 (31) and dlg m52 -FRT101. Follicle cell clones were generated with hsFLP stocks carrying ubiquitin-GFP (30) recombined onto FRT101 FRT40 and FRT82B chromosomes.
Embryos were fixed (1) and stained with anti-Arm anti-Nrt and anti-FasIII supernatants from the Developmental Studies Hybridoma Bank; anti-Crb from M. Bhat; anti-Dlg from P. Bryant; and anti-Lgl from D. Strand. Fluorescent images are single sections collected with a Lecia TCS confocal microscope.
Non-Tubby larvae were selected from scrib 1 /TM6B Tb stocks and fixed in 4% formaldehyde for tissue staining. Because overgrown scrib discs are often fused together such that individual disc identities cannot be ascertained all thoracic discs from one side of 10 late third instar (assayed by larval size and eversion of anterior spiracles) WT and scrib larvae were dissociated and counted on a hemocytometer essentially as described in P. F. Martin [ J. Exp. Zool 222 97 (1982)].
Gateff E., Schneiderman H. A., Natl. Cancer Inst. Monogr. 31, 365 (1969).
Strand D., et al., Oncogene 11, 291 (1995).
GenBank number NM−004524
We thank M. Bender P. Bryant B. Mechler J. K. Roy A. Shearn D. Strand D. Strutt and D. Woods for generously providing materials and B. Mathey-Prevot and A. Chen for comments on the manuscript. D.B is a Fellow of the American Cancer Society.
