Regional Changes in Calcium Underlying Contraction of Single Smooth Muscle Cells

American Association for the Advancement of Science (AAAS) - Tập 235 Số 4796 - Trang 1644-1648 - 1987
David A. Williams1, Peter L. Becker1, Frederic S. Fay1
1Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01605.

Tóm tắt

The role of calcium in regulating the contractile state of smooth muscle has been investigated by measuring calcium and contraction in single smooth muscle cells with the calcium-sensitive dye fura-2 and the digital imaging microscope. The concentration of free calcium in the cytoplasm increased after stimulation of the cells by depolarization with high potassium or by application of carbachol. Changes in calcium always preceded contraction. The increase in calcium induced by these stimuli was limited to less than 1 μ M . Calcium within the nucleus was also subject to a limitation of its rise during contraction. Intranuclear calcium rose from 200 n M at rest to no more than 300 n M while cytoplasmic calcium rose to over 700 n M . These apparent ceilings for both cytoplasmic and intranuclear calcium may result either from negative feedback of calcium on cytoplasmic and nuclear calcium channel gating mechanisms, respectively, or from the presence of calcium pumps that are strongly activated at the calcium ceilings.

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Tài liệu tham khảo

ABRAMSON S.B. unpublished data.

BECKER, P. L., JOURNAL OF GENERAL PHYSIOLOGY 88: A11 (1986).

BECKER P.L. unpublished data.

10.1126/science.3704641

BOND, M, JOURNAL OF PHYSIOLOGY-LONDON 357: 185 (1984).

Clapp, L. H., Proceedings of the National Academy of Sciences of the United States of America 84: 2092 (1987).

DEFEO, T.T., JOURNAL OF PHYSIOLOGY-LONDON 369: 269 (1985).

ECKERT, R, INACTIVATION OF CA CHANNELS, PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY 44: 215 (1984).

FABIATO, A, ACTA PHYSIOLOGICA SCANDINAVICA 508: 16 (1982).

FABIATO, A, CALCIUM-INDUCED RELEASE OF CALCIUM FROM THE CARDIAC SARCOPLASMIC-RETICULUM, AMERICAN JOURNAL OF PHYSIOLOGY 245: C1 (1983).

FAY, F.S., PREPARATION OF INDIVIDUAL SMOOTH-MUSCLE CELLS FROM THE STOMACH OF BUFO-MARINUS, METHODS IN ENZYMOLOGY 85: 284 (1982).

FAY, F.S., ISOMETRIC CONTRACTILE PROPERTIES OF SINGLE ISOLATED SMOOTH-MUSCLE CELLS, NATURE 265: 553 (1977).

FAY, F.S., AEQUORIN LUMINESCENCE DURING ACTIVATION OF SINGLE ISOLATED SMOOTH-MUSCLE CELLS, NATURE 280: 506 (1979).

FAY, F.S., OPTICAL METHODS CELL: 51 (1986).

GROVER, A.K., CA-PUMPS IN SMOOTH-MUSCLE - ONE IN PLASMA-MEMBRANE AND ANOTHER IN ENDOPLASMIC-RETICULUM, CELL CALCIUM 6: 227 (1985).

10.1016/S0021-9258(19)83641-4

HATCH, J.F., HIGH TIME RESOLUTION MEASUREMENTS OF [CA++] IN SINGLE SMOOTH-MUSCLE CELLS USING FURA-2 AND AN ULTRA-FAST DIGITAL MICROFLUORIMETER, BIOPHYSICAL JOURNAL 49: A464 (1986).

KAMM, K.E., THE FUNCTION OF MYOSIN AND MYOSIN LIGHT CHAIN KINASE PHOSPHORYLATION IN SMOOTH-MUSCLE, ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY 25: 593 (1985).

Kargacin, G. J., Journal of General Physiology 90: 49 (1987).

10.1126/science.3927484

MAIZELS, E.T., CA2+-CALMODULIN-DEPENDENT PHOSPHORYLATION OF SOLUBLE AND NUCLEAR PROTEINS IN THE RAT OVARY, ENDOCRINOLOGY 112: 1895 (1983).

MORGAN, J.P., STIMULUS-SPECIFIC PATTERNS OF INTRACELLULAR CALCIUM LEVELS IN SMOOTH-MUSCLE OF FERRET PORTAL-VEIN, JOURNAL OF PHYSIOLOGY-LONDON 351: 155 (1984).

MORGAN, J.P., VASCULAR SMOOTH-MUSCLE - THE 1ST RECORDED CA-2+ TRANSIENTS, PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY 395: 75 (1982).

NEERING, I.R., USE OF AEQUORIN TO STUDY EXCITATION-CONTRACTION COUPLING IN MAMMALIAN SMOOTH-MUSCLE, NATURE 288: 585 (1980).

REMBOLD, C.M., MYOPLASMIC CALCIUM, MYOSIN PHOSPHORYLATION, AND REGULATION OF THE CROSSBRIDGE CYCLE IN SWINE ARTERIAL SMOOTH-MUSCLE, CIRCULATION RESEARCH 58: 803 (1986).

SAHYOUN, N, CA-2+-CALMODULIN-DEPENDENT PROTEIN-KINASE IN NEURONAL NUCLEI, JOURNAL OF BIOLOGICAL CHEMISTRY 259: 9341 (1984).

Sahyoun, N., Proceedings of the National Academy of Sciences of the United States of America 81: 4311 (1984).

SCANLON, M, CA++-INSENSITIVE INTERMEDIATE IN THE INTRACELLULAR CLEAVAGE OF FURA-2, JOURNAL OF GENERAL PHYSIOLOGY 88: A52 (1986).

SCHEID, C.R., TRANSMEMBRANE CA-45 FLUXES IN ISOLATED SMOOTH-MUSCLE CELLS - BASAL CA-2+ FLUXES, AMERICAN JOURNAL OF PHYSIOLOGY 246: C422 (1984).

TILLOTSON, D, INACTIVATION OF CA CONDUCTANCE DEPENDENT ON ENTRY OF CA IONS IN MOLLUSCAN NEURONS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 76: 1497 (1979).

WALSH, J.V., CALCIUM ACTION-POTENTIALS IN SINGLE FRESHLY ISOLATED SMOOTH-MUSCLE CELLS, AMERICAN JOURNAL OF PHYSIOLOGY 239: C162 (1980).

WHITE, B.A., EVIDENCE FOR A ROLE OF CALMODULIN IN THE REGULATION OF PROLACTIN GENE-EXPRESSION, JOURNAL OF BIOLOGICAL CHEMISTRY 260: 1213 (1985).

WILLIAMS, D.A., AM J PHYSIOL 250: C799 (1986).

WILLIAMS, D.A., MAXIMAL CELL SHORTENING IN INTACT SMOOTH-MUSCLE REQUIRES RELATIVELY SMALL CHANGES IN CYTOPLASMIC CA++ - MEASUREMENTS OF FURA-2 FLUORESCENCE IN SINGLE CELLS, BIOPHYSICAL JOURNAL 49: A464 (1986).

WILLIAMS, D.A., LOCALIZATION OF CA++ IN SINGLE SMOOTH-MUSCLE CELLS - THE USE OF DIGITAL IMAGING MICROSCOPY AND FURA-2, JOURNAL OF GENERAL PHYSIOLOGY 86: A37 (1985).

WILLIAMS, D.A., CA-2+ AND FURA-2 IN THE NUCLEAR ENVIRONMENT OF ISOLATED SMOOTH-MUSCLE CELLS, JOURNAL OF GENERAL PHYSIOLOGY 88: A61 (1986).

10.1038/318558a0

WILLIAMS D.A. unpublished data.

YAMAGUCHI, H, RECORDING OF INTRACELLULAR CA-2+ FROM SMOOTH-MUSCLE CELLS BY SUBMICRON TIP, DOUBLE-BARRELED CA-2+-SELECTIVE MICROELECTRODES, CELL CALCIUM 7: 203 (1986).

ZIMMERMANN, U, BIOTECHNIQUES 1: 118 (1983).

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American Association for the Advancement of Science (AAAS)

Tập 235 Số 4796

1644-1648

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