Cytotoxicity of four Aframomum species (A. arundinaceum, A. alboviolaceum, A. kayserianum and A. polyanthum) towards multi-factorial drug resistant cancer cell lines
Tóm tắt
The search for natural products as potential cytotoxic agents has yielded promising candidates. However multidrug resistance (MDR) is still a major hurdle for patients receiving chemotherapy. In the present study, we evaluated the cytotoxicity of the methanol extracts of four dietary Aframomum plant species (A. arundinaceum, A. alboviolaceum, A. kayserianum and A. polyanthum) against nine sensitive and MDR cancer cell lines. We have also identified the bioactive constituents of A. arundinaceum. The cytotoxicity of the methanol extracts of the above plants was determined using a resazurin reduction assay. Chromatographic techniques were used to isolate the constituents of A. arundinaceum.
A preliminary experiment on leukemia CCRF-CEM cells at 40 μg/mL showed that the extracts from A. kayserianum and A. alboviolaceum as well as the isolated compounds namely compounds aframodial (1), 8(17),12-labdadien-15,16-dial (2), galanolactone (3), 1-p-menthene-3,6-diol (6) and 1,4-dimethoxybenzene (7) were less active, inducing more than 50% growth of this cell line contrary to A. polyanthum and A. arundinaceum extracts, galanals A (4) and B (5), naringenin (8) and kaempferol-3,7,4’-trimethylether (9). The IC50 values below or around 30 μg/mL were recorded with A. arundinaceum extract against eight of the nine tested cancer cell lines. This extract as well as compound 8 displayed IC50 values below 40 μg/mL towards the nine tested cancer cell lines whilst A. polyanthum extract, compounds 4, 5 and 9 showed selective activities. Collateral sensitivity (hypersensitivity) was observed with A. arundinaceum extract towards leukemia CEM/ADR5000 cells and glioblastoma U87MG.ΔEGFR compared to their respective sensitive counterparts CEM/CEM and U87MG. The results of this study provide evidence of the cytotoxicity selected Aframomum species as well as a baseline information for the potential use of Aframomum arundinaceum in the fight against drug sensitive and otherwise drug-resistant cancers.
Tài liệu tham khảo
Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D: Global cancer statistics. CA-Cancer J Clin. 2011, 61 (2): 69-90. 10.3322/caac.20107.
Chen Z, Zhang L, Xia L, Jin Y, Wu Q, Guo H, Shang X, Dou J, Wu K, Nie Y, Fan D: Genomic analysis of drug resistant gastric cancer cell lines by combining mRNA and microRNA expression profiling. Cancer Lett. 2014, 350 (1–2): 43-51.
Marchini S, Marrazzo E, Bonomi R, Chiorino G, Zaffaroni M, Weissbach L, Hornicek FJ, Broggini M, Faircloth GT, D’Incalci M: Molecular characterisation of two human cancer cell lines selected in vitro for their chemotherapeutic drug resistance to ET-743. Eur J Cancer. 2005, 41 (2): 323-333. 10.1016/j.ejca.2004.10.021.
Kuete V, Mbaveng AT, Tsaffack M, Beng VP, Etoa FX, Nkengfack AE, Meyer JJ, Lall N: Antitumor, antioxidant and antimicrobial activities of Bersama engleriana (Melianthaceae). J Ethnopharmacol. 2008, 115 (3): 494-501. 10.1016/j.jep.2007.10.027.
Kuete V, Krusche B, Youns M, Voukeng I, Fankam AG, Tankeo S, Lacmata S, Efferth T: Cytotoxicity of some Cameroonian spices and selected medicinal plant extracts. J Ethnopharmacol. 2011, 134 (3): 803-812. 10.1016/j.jep.2011.01.035.
Kuete V, Sandjo L, Nantchouang Ouete J, Fouotsa H, Wiench B, Efferth T: Cytotoxicity and modes of action of three naturally occuring xanthones (8-hydroxycudraxanthone G, morusignin I and cudraxanthone I) against sensitive and multidrug-resistant cancer cell lines. Phytomedicine. 2013, 21 (3): 315-322.
Kuete V, Sandjo LP, Kwamou GM, Wiench B, Nkengfack AE, Efferth T: Activity of three cytotoxic isoflavonoids from Erythrina excelsa and Erythrina senegalensis (neobavaisoflavone, sigmoidin H and isoneorautenol) toward multi-factorial drug resistant cancer cells. Phytomedicine. 2014, 21 (5): 682-688. 10.1016/j.phymed.2013.10.017.
Goldstein LJ, Galski H, Fojo A, Willingham M, Lai S-L, Gazdar A, Pirker R, Green A, Crist W, Brodeur GM, Lieber M, Cossman J, Gottesman MM, Pastan I: Expression of multidrug resistance gene in human cancers. J Natl Cancer Inst. 1989, 81 (2): 116-124. 10.1093/jnci/81.2.116.
Kuwazuru Y, Yoshimura A, Hanada S, Ichikawa M, Saito T, Uozumi K, Utsunomiya A, Arima T, Akiyama S-I: Expression of the multidrug transporter, P-glycoprotein, in chronic myelogenous leukaemia cells in blast crisis. Brit J Haematol. 1990, 74 (1): 24-29. 10.1111/j.1365-2141.1990.tb02533.x.
Tane P, Tatsimo S, Ayimele G, Connolly J: Bioactive metabolites from Aframomum species. 11th NAPRECA Symposium Book of Proceedings. 2005, Antananarivo, Madagascar: NAPRECA, 214-223.
Dzoyem JP, Guru SK, Pieme CA, Kuete V, Sharma A, Khan IA, Saxena AK, Vishwakarma RA: Cytotoxic and antimicrobial activity of selected Cameroonian edible plants. BMC Complement Altern Med. 2013, 13: 78-10.1186/1472-6882-13-78.
Djeussi DE, Noumedem JA, Seukep JA, Fankam AG, Voukeng IK, Tankeo SB, Nkuete AH, Kuete V: Antibacterial activities of selected edible plants extracts against multidrug-resistant Gram-negative bacteria. BMC Complement Altern Med. 2013, 13 (1): 164-10.1186/1472-6882-13-164.
Seukep JA, Fankam AG, Djeussi DE, Voukeng IK, Tankeo SB, Noumdem JA, Kuete AH, Kuete V: Antibacterial activities of the methanol extracts of seven Cameroonian dietary plants against bacteria expressing MDR phenotypes. Springerplus. 2013, 2: 363-10.1186/2193-1801-2-363.
Thomas D, Thomas J, Bromley W, Mbenkum F: Korup ethnobotany survey, final report to: The World Wide Fund for Nature. 1989, Weyside Park, Godalming, Surrey, UK: Penda House
Ndukwu B, Ben-Nwadibia N: Ethnomedicinal aspects of plants used as spices and condiments in the Niger delta area of Nigeria. 2010, Port Harcourt, Nigeria: University of Port Harcourt PMB
Iwu M: Handbook of African Medicinal Plants. 1993, Boca Raton, FL: CRC Press
Duker-Eshun G, Jaroszewski JW, Asomaning WA, Oppong-Boachie F, Olsen CE, Christensen SB: Antiplasmodial activity of labdanes from Aframomum latifolium and Aframomum sceptrum. Planta Med. 2002, 68 (7): 642-644. 10.1055/s-2002-32888.
O’Brien J, Wilson I, Orton T, Pognan F: Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem. 2000, 267 (17): 5421-5426. 10.1046/j.1432-1327.2000.01606.x.
Kuete V, Tchakam PD, Wiench B, Ngameni B, Wabo HK, Tala MF, Moungang ML, Ngadjui BT, Murayama T, Efferth T: Cytotoxicity and modes of action of four naturally occuring benzophenones: 2,2′,5,6′-tetrahydroxybenzophenone, guttiferone E, isogarcinol and isoxanthochymol. Phytomedicine. 2013, 20 (6): 528-536. 10.1016/j.phymed.2013.02.003.
Kimmig A, Gekeler V, Neumann M, Frese G, Handgretinger R, Kardos G, Diddens H, Niethammer D: Susceptibility of multidrug-resistant human leukemia cell lines to human interleukin 2-activated killer cells. Cancer Res. 1990, 50 (21): 6793-6799.
Efferth T, Sauerbrey A, Olbrich A, Gebhart E, Rauch P, Weber HO, Hengstler JG, Halatsch ME, Volm M, Tew KD, Ross DD, Funk JO: Molecular modes of action of artesunate in tumor cell lines. Mol Pharmacol. 2003, 64 (2): 382-394. 10.1124/mol.64.2.382.
Gillet J, Efferth T, Steinbach D, Hamels J, de Longueville F, Bertholet V, Remacle J: Microarray-based detection of multidrug resistance in human tumor cells by expression profiling of ATP-binding cassette transporter genes. Cancer Res. 2004, 64 (24): 8987-8993. 10.1158/0008-5472.CAN-04-1978.
Doyle LA, Yang W, Abruzzo LV, Krogmann T, Gao Y, Rishi AK, Ross DD: A multidrug resistance transporter from human MCF-7 breast cancer cells. Proc Natl Acad Sci U S A. 1998, 95 (26): 15665-15670. 10.1073/pnas.95.26.15665.
Kuete V, Sandjo L, Wiench B, Efferth T: Cytotoxicity and modes of action of four Cameroonian dietary spices ethno-medically used to treat Cancers: Echinops giganteus, Xylopia aethiopica, Imperata cylindrica and Piper capense. J Ethnopharmacol. 2013, 149 (1): 245-253. 10.1016/j.jep.2013.06.029.
Kuete V, Fankam AG, Wiench B, Efferth T: Cytotoxicity and modes of action of the methanol extracts of six Cameroonian medicinal plants against multidrug-resistant tumor cells. Evid Based Complement Alternat Med. 2013, 2013: 285903-
Kuete V, Tankeo SB, Saeed ME, Wiench B, Tane P, Efferth T: Cytotoxicity and modes of action of five Cameroonian medicinal plants against multi-factorial drug resistance of tumor cells. J Ethnopharmacol. 2014, 153 (1): 207-219. 10.1016/j.jep.2014.02.025.
Kamdem Wabo H, Tane P, Connolly J: Diterpenoids and sesquiterpenoids from Aframomum arundinaceum. Biochem Syst Ecol. 2006, 34: 603-605. 10.1016/j.bse.2006.02.001.
Morita H, Itokawa H: Cytotoxic and antifungal diterpenes from the seeds of Alpinia galanga. Planta Med. 1988, 54 (2): 117-120. 10.1055/s-2006-962365.
Miyoshi N, Nakamura Y, Ueda Y, Abe M, Ozawa Y, Uchida K, Osawa T: Dietary ginger constituents, galanals A and B, are potent apoptosis inducers in Human T lymphoma Jurkat cells. Cancer Lett. 2003, 199 (2): 113-119. 10.1016/S0304-3835(03)00381-1.
Bousetla A, Konuklugil B, Bouacida S, Zellagui A, Rhouati S, Akkal S: Phytochemical study of Algerian Foeniculum vulgare Mill (Apiaceae). Der Pharmacia Lettre. 2013, 5 (6): 9-11.
Rogerson FSS, Azevedo Z, Fortunato N, de Freitas VAP: 1,3-Dimethoxybenzene, a newly identified component of port wine. J Sci Food Agric. 2002, 82 (11): 1287-1292. 10.1002/jsfa.1182.
Nilupa R, Lalith J, Noriyuki H, Yoshinori F: Chemical constituents of the fruits of Artocarpus altilis. Biochem Syst Ecol. 2007, 36: 323-325.
Pizzolatti M, Verdi L, Brighente I, Neiva T, Schripsema J, Braz Filho R: Anticoagulant effect and constituents of Baccharis illinita. Nat Prod Commun. 2006, 1 (1): 37-42.
Suffness M, Pezzuto J: Assays related to cancer drug discovery. 1990, London: Academic Press
Boik J: Natural compounds in cancer therapy. 2001, Minnesota USA: Oregon Medical Press
Brahemi G, Kona FR, Fiasella A, Buac D, Soukupova J, Brancale A, Burger AM, Westwell AD: Exploring the structural requirements for inhibition of the ubiquitin E3 ligase breast cancer associated protein 2 (BCA2) as a treatment for breast cancer. J Med Chem. 2010, 53 (7): 2757-2765. 10.1021/jm901757t.
Efferth T: The human ATP-binding cassette transporter genes: from the bench to the bedside. Curr Mol Med. 2001, 1 (1): 45-65. 10.2174/1566524013364194.
Gottesman MM, Ling V: The molecular basis of multidrug resistance in cancer: the early years of P-glycoprotein research. FEBS Lett. 2006, 580 (4): 998-1009. 10.1016/j.febslet.2005.12.060.
Gillet JP, Efferth T, Remacle J: Chemotherapy-induced resistance by ATP-binding cassette transporter genes. Biochim Biophys Acta. 2007, 1775 (2): 237-262.
Chen YC, Shen SC, Lin HY: Rutinoside at C7 attenuates the apoptosis-inducing activity of flavonoids. Biochem Pharmacol. 2003, 66 (7): 1139-1150. 10.1016/S0006-2952(03)00455-6.
Kanno S, Shouji A, Asou K, Ishikawa M: Effects of naringin on hydrogen peroxide-induced cytotoxicity and apoptosis in P388 cells. J Pharmacol Sci. 2003, 92 (2): 166-170. 10.1254/jphs.92.166.
Wang BD, Yang ZY, Wang Q, Cai TK, Crewdson P: Synthesis, characterization, cytotoxic activities, and DNA-binding properties of the La(III) complex with Naringenin Schiff-base. Bioorg Med Chem. 2006, 14 (6): 1880-1888. 10.1016/j.bmc.2005.10.031.
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