Elamipretide effects on the skeletal muscle phosphoproteome in aged female mice
Tóm tắt
The age-related decline in skeletal muscle mass and function is known as sarcopenia. Sarcopenia progresses based on complex processes involving protein dynamics, cell signaling, oxidative stress, and repair. We have previously found that 8-week treatment with elamipretide improves skeletal muscle function, reverses redox stress, and restores protein S-glutathionylation changes in aged female mice. This study tested whether 8-week treatment with elamipretide also affects global phosphorylation in skeletal muscle consistent with functional improvements and S-glutathionylation. Using female 6–7-month-old mice and 28–29-month-old mice, we found that phosphorylation changes did not relate to S-glutathionylation modifications, but that treatment with elamipretide did partially reverse age-related changes in protein phosphorylation in mouse skeletal muscle.
Tài liệu tham khảo
Lopez-Otin C, Blasco MA, Partridge L, Serrano M, Kroemer G. The hallmarks of aging. Cell. 2013;153:1194–217.
McCormick R, Vasilaki A. Age-related changes in skeletal muscle: changes to life-style as a therapy. Biogerontology. 2018;19:519–36.
Metter EJ, Conwit R, Tobin J, Fozard JL. Age-associated loss of power and strength in the upper extremities in women and men. J Gerontol A Biol Sci Med Sci. 1997;52:B267-276.
Marzetti E, Lees HA, Wohlgemuth SE, Leeuwenburgh C. Sarcopenia of aging: underlying cellular mechanisms and protection by calorie restriction. BioFactors. 2009;35:28–35.
Jang YC, et al. Increased superoxide in vivo accelerates age-associated muscle atrophy through mitochondrial dysfunction and neuromuscular junction degeneration. FASEB J. 2010;24:1376–90.
Deepa SS, et al. Accelerated sarcopenia in Cu/Zn superoxide dismutase knockout mice. Free Radic Biol Med. 2019;132:19–23.
Amara CE, et al. Mild mitochondrial uncoupling impacts cellular aging in human muscles in vivo. Proc Natl Acad Sci U S A. 2007;104:1057–62.
Conley KE, Jubrias SA, Esselman PC. Oxidative capacity and ageing in human muscle. J Physiol. 2000;526(Pt 1):203–10.
Buxbaum JD, Chernew ME, Fendrick AM, Cutler DM. Contributions of public health, pharmaceuticals, and other medical care to US life expectancy changes, 1990–2015. Health Aff (Millwood). 2020;39:1546–56.
Birk AV, et al. The mitochondrial-targeted compound SS-31 re-energizes ischemic mitochondria by interacting with cardiolipin. J Am Soc Nephrol. 2013;24:1250–61.
Chavez JD, et al. Mitochondrial protein interaction landscape of SS-31. Proc Natl Acad Sci U S A. 2020;117:15363–73.
Siegel MP, et al. Mitochondrial-targeted peptide rapidly improves mitochondrial energetics and skeletal muscle performance in aged mice. Aging Cell. 2013;12:763–71.
Campbell MD, et al. Improving mitochondrial function with SS-31 reverses age-related redox stress and improves exercise tolerance in aged mice. Free Radic Biol Med. 2019;134:268–81.
Chiao YA, et al. Late-life restoration of mitochondrial function reverses cardiac dysfunction in old mice. Elife. 2020;9:e55513.
Whitson JA, et al. SS-31 and NMN: two paths to improve metabolism and function in aged hearts. Aging Cell. 2020;19: e13213.
Alam NM, Douglas RM, Prusky GT. Treatment of age-related visual impairment with a peptide acting on mitochondria. Dis Model Mech. 2022;15:dmm048256.
Tarantini S, et al. Treatment with the mitochondrial-targeted antioxidant peptide SS-31 rescues neurovascular coupling responses and cerebrovascular endothelial function and improves cognition in aged mice. Aging Cell. 2018;17:e12731.
Sweetwyne MT, et al. The mitochondrial-targeted peptide, SS-31, improves glomerular architecture in mice of advanced age. Kidney Int. 2017;91:1126–45.
Baumann CW, Kwak D, Liu HM, Thompson LV. Age-induced oxidative stress: how does it influence skeletal muscle quantity and quality? J Appl Physiol. 2016;1985(121):1047–52.
Musaogullari A, Chai YC. Redox regulation by protein S-glutathionylation: from molecular mechanisms to implications in health and disease. Int J Mol Sci. 2020;21:8113.
Whitson JA, et al. Elamipretide (SS-31) treatment attenuates age-associated post-translational modifications of heart proteins. Geroscience. 2021;43:2395–412.
Martin-Perez M, et al. PKC downregulation upon rapamycin treatment attenuates mitochondrial disease. Nat Metab. 2020;2:1472–81.
Egertson JD, MacLean B, Johnson R, Xuan Y, MacCoss MJ. Multiplexed peptide analysis using data-independent acquisition and Skyline. Nat Protoc. 2015;10:887–903.
Xie Y, Kole S, Precht P, Pazin MJ, Bernier M. S-Glutathionylation impairs signal transducer and activator of transcription 3 activation and signaling. Endocrinology. 2009;150:1122–31.
Budde H, et al. The interplay between S-glutathionylation and phosphorylation of cardiac troponin I and myosin binding protein C in end-stage human failing hearts. Antioxidants (Basel). 2021;10:1134.
Sweeney HL, Bowman BF, Stull JT. Myosin light chain phosphorylation in vertebrate striated muscle: regulation and function. Am J Physiol. 1993;264:C1085-1095.
Zhao ZZ, Malencik DA, Anderson SR. Protein-induced inactivation and phosphorylation of rabbit muscle phosphofructokinase. Biochemistry. 1991;30:2204–16.
Fischer M, Rikeit P, Knaus P, Coirault C. YAP-mediated mechanotransduction in skeletal muscle. Front Physiol. 2016;7:41.
Hamdani N, Herwig M, Linke WA. Tampering with springs: phosphorylation of titin affecting the mechanical function of cardiomyocytes. Biophys Rev. 2017;9:225–37.
Alegre-Cebollada J, et al. S-Glutathionylation of cryptic cysteines enhances titin elasticity by blocking protein folding. Cell. 2014;156:1235–46.
McCalmon SA, et al. Modulation of angiotensin II-mediated cardiac remodeling by the MEF2A target gene Xirp2. Circ Res. 2010;106:952–60.
Cherepanova O, et al. Xin-repeats and nebulin-like repeats bind to F-actin in a similar manner. J Mol Biol. 2006;356:714–23.
Pacholsky D, et al. Xin repeats define a novel actin-binding motif. J Cell Sci. 2004;117:5257–68.
Huang HT, et al. Myomaxin is a novel transcriptional target of MEF2A that encodes a Xin-related alpha-actinin-interacting protein. J Biol Chem. 2006;281:39370–9.
Otten C, et al. Xirp proteins mark injured skeletal muscle in zebrafish. PLoS One. 2012;7: e31041.
Graham ZA, DeBerry JJ, Cardozo CP, Bamman MM. SS-31 does not prevent or reduce muscle atrophy 7 days after a 65 kdyne contusion spinal cord injury in young male mice. Physiol Rep. 2022;10: e15266.