No transmission of hepatitis E virus in pigs fed diets containing commercial spray-dried porcine plasma: a retrospective study of samples from several swine trials
Tóm tắt
Hepatitis E virus (HEV) has been reported in the human population and pigs are a recognized reservoir for HEV and a possible source of HEV transmission to humans. Spray-dried porcine plasma (SDPP) is an ingredient commonly used in feed for pigs around the world. Even though processing conditions used to produce SDPP should be adequate to inactivate HEV, it was of interest to analyze commercial SDPP samples for presence of genome and antibodies (AB) against HEV and to retrospectively analyze serum samples collected from pigs used in past experiments that had been fed diets containing either 0% or 8% SDPP to detect potential transmission of HEV as determined by seroconversion. Eighty-five commercial SDPP samples were analyzed by ELISA and 100% of them contained AB against HEV, while 22.4% (11 of 49 samples analyzed) were positive for HEV RNA. Frozen sera samples (n = 140) collected from 70 pigs used in past experiments that had been fed diets containing either 0% or 8% commercial SDPP was analyzed by ELISA for AB against HEV. Age of pigs at sera sampling ranged from 3 to 15 weeks and feeding duration of diets ranged from approximately 4 to 9 weeks. One lot of SDPP used in one experiment was analyzed and confirmed to contain HEV RNA. Regardless of the diet fed, some sera samples collected at the beginning of an experiment contained AB titer against HEV. These sera samples were collected from weaned pigs prior to feeding of the experimental diets and the HEV titer was probably from maternal origin. However, by the end of the experiments, HEV titer was not detected or had declined by more than 50% of the initial titer concentration. To our knowledge, this is the first study reporting presence of HEV AB titer and RNA in SDPP. Retrospective analysis of serum collected from pigs fed diets with SDPP revealed no indication of seroconversion to HEV. The results indicate that feeding SDPP in diets for pigs does not represent a risk of transmitting HEV, even though HEV genome may be detected in SDPP.
Tài liệu tham khảo
Van Dijk AJ, Everts H, Nabuurs MJA, Margry RJCF, Beynen AC: Growth performance of weanling pigs fed spray-dried animal plasma: a review. Livest Prod Sci 2001, 68: 263-274. 10.1016/S0301-6226(00)00229-3
Torrallardona D: Spray dried animal plasma as an alternative to antibiotics in weanling pigs- a review -. Asian Aust J Anim Sci 2010, 23: 131-148. 10.5713/ajas.2010.70630
Peace RM, Campbell J, Polo J, Crenshaw J, Russell L, Moeser A: Spray-dried porcine plasma influences intestinal barrier function, inflammation and diarrhea in weaned pigs. J Nutr 2011, 141: 1312-1317. 10.3945/jn.110.136796
Dalton HR, Bendall R, Ijaz S, Banks M: Hepatitis E: an emerging infection in developed countries. Lancet Infect Dis 2008, 8: 698-709. 10.1016/S1473-3099(08)70255-X
Pavio N, Renou C, Di Liberto G, Boutrouille A, Eloit M: Hepatitis E: a curious zoonosis. Front Biosci 2008, 13: 7172-7183. 10.2741/3219
Masia G, Orru G, Liciardi M, Desogus G, Coppola RC, Murru V, Argiolas M: Evidence of hepatitis E virus (HEV) infection in human and pigs in Sardinia, Italy. J Prev Med Hyg 2009, 50: 227-231.
Purcell RH, Emerson SU: Hepatitis E: an emerging awareness of an old disease. J Hepatol 2008, 48: 494-503. 10.1016/j.jhep.2007.12.008
Colson P, Borentain P, Queyriaux B, Kaba M, Moal V, Gallian P: Pig liver sausage as a source of hepatitis E virus transmission to humans. J Infect Dis 2010, 202: 825-834. 10.1086/655898
Meng XJ: From barnyard to food table: the omnipresence of hepatitis E virus and risk for zoonotic infection and food safety. Virus Res 2011,161(1):23-30. 10.1016/j.virusres.2011.01.016
Pavio N, Meng XJ, Renou C: Zoonotic hepatitis E: animal reservoirs and emerging risks. Vet Res 2010, 41: 46. 10.1051/vetres/2010018
Kaba M, Davoust B, Marie JL, Colson P: Detection of hepatitis E virus in wild boar (Sus scrofa) livers. Vet J 2010,186(2):259-261. doi: 10.1016/j.tvjl.2009.08.008. Epub 2009 Sep 10 10.1016/j.tvjl.2009.08.008
De Deus N, Seminati C, Pina S, Mateu E, Martín M, Segalés J: Detection of hepatitis E virus in liver, mesenteric lymph node, serum, bile and faeces of naturally infected pigs affected by different pathological conditions. Vet Microbiol 2007,119(2–4):105-114. 10.1016/j.vetmic.2006.08.027
Pischke S, Potthoff A, Hauröder B, Schlué J, Manns MP, Cornberg M, Wedemeyer H: Hepatitis E virus infection: a paradigm shift? Dtsch Med Wochenschr 2010, 135: 1129-1133. 10.1055/s-0030-1255136
Christou L, Kosmidou M: Hepatitis E virus in the Western world—a pork-related zoonosis. Clin Microbiol Infect 2014, 19: 600-604. 10.1111/1469-0691.12214
Emerson SU, Arankalle VA, Purcell RH: Thermal stability of hepatitis E virus. J Infect Dis 2005, 192: 930-933. 10.1086/432488
Berto A, Backer JA, Mesquita JR, Nascimento MSJ, Banks M, Martelli F, Ostanello F, Angeloni G, Di Bartolo I, Ruggeri FM, Vasickova P, Diez-Valcarce M, Hernandez M, Rodriguez-Lazaro D, Van der Poel WHM: Prevalence and transmission of hepatitis E virus in domestic swine populations in different European countries. BMC Res Notes 5:190. doi:10.1186/1756-0500-5-190.
Casas M, Pujols J, Rosell R, De Deus N, Peralta B, Pina S, Casal J, Martín M: Retrospective serological study on hepatitis E infection in pigs from 1985 to 1997 in Spain. Vet Microbiol 2009, 135: 248-252. 10.1016/j.vetmic.2008.09.075
Jiménez de Oya N, De Blas I, Blázquez AB, Martín-Acebes MA, Halaihel N, Gironés O, Saiz JC, Escribano-Romero E: Widespread distribution of hepatitis E virus in Spanish pig herds. BMC Res Notes 2011, 4: 412. 10.1186/1756-0500-4-412
Seminati C, Mateu E, Peralta B, De Deus N, Martín M: Distribution of hepatitis E virus infection and its prevalence in pigs on commercial farms in Spain. Vet J 2008, 175: 130-132. 10.1016/j.tvjl.2006.11.018
De Deus N, Casas M, Peralta B, Nofrarias M, Pina S, Martín M, Segalés J: Hepatitis E virus infection dynamics and organic distribution in naturally infected pigs in a farrow-to-finish farm. Vet Microbiol 2008, 132: 19-28. 10.1016/j.vetmic.2008.04.036
Breum SØ, Hjulsager CK, De Deus N, Segalés J, Larsen LE: Hepatitis E virus is highly prevalent in the Danish pig population. Vet Microbiol 2010,146(1–2):144-149. 10.1016/j.vetmic.2010.05.002
Borg BS, Campbell JM, Polo J, Russell LE, Rodriquez C, Rodenas J: Evaluation of the Chemical and Biological Characteristics of Spray-Dried Plasma Protein Collected from Various Locations Around the World. Proc Amer Assoc Swine Vet 2002, 97-100.
Polo J, Opriessnig T, O’Neill KC, Rodríguez C, Russell LE, Campbell JM, Crenshaw J, Segalés J, Pujols J: Neutralizing antibodies against porcine circovirus type 2 (PCV2) in liquid pooled plasma contribute to the bio-safety of commercially manufactured spray-dried porcine plasma. J Anim Sci 2013, 91: 2192-2198. 10.2527/jas.2012-5705
Nakai I, Kato K, Miyazaki A, Yoshii M, Li TC, Takeda N, Tsunemitsu H, Ikeda H: Different fecal shedding patterns of two common strains of hepatitis E virus at three Japanese swine farms. Am J Trop Med Hyg 2006, 75: 1171-1177.
Takahashi M, Nishizawa T, Tanaka T, Tsatsralt-Od B, Inoue J, Okamoto H: Correlation between positivity for immunoglobulin A antibodies and viraemia of swine hepatitis E virus observed among farm pigs in Japan. J Gen Virol 2005, 86: 1807-1813. 10.1099/vir.0.80909-0
Casas M, Cortés R, Pina S, Peralta B, Allepuz A, Cortey M, Casal J, Martín M: Longitudinal study of hepatitis E virus infection in Spanish farrow-to-finish swine herds. Vet Microbiol 2011, 148: 27-34. 10.1016/j.vetmic.2010.08.010
Chandra V, Taneja S, Kalia M, Jameel S: Molecular biology and pathogenesis of hepatitis E virus. J Biosci 2008, 33: 451-464. 10.1007/s12038-008-0064-1
Huang R, Li D, Wei S, Li Q, Yuan X, Geng L, Li X, Liu M: Cell culture of sporadic hepatitis E virus in China. Clin Diag Lab Immun 1999, 6: 729-733.
Feagins AR, Opriessnig T, Guenette DK, Halbur PG, Meng XJ: Inactivation of infectious hepatitis E virus present in commercial pig livers sold in local grocery stores in the United States. Int J Food Microbiol 2008, 123: 32-37. 10.1016/j.ijfoodmicro.2007.11.068
Barnaud E, Rogée S, Garry P, Rose N, Pavio N: Thermal inactivation of infectious hepatitis E virus in experimentally contaminated food. Appl Environ Microbiol 2012, 78: 5153-5159. 10.1128/AEM.00436-12
Polo J, Quigley JD, Russell LE, Campbell JM, Pujols J, Lukert PD: Efficacy of spray-drying to reduce infectivity of pseudo rabies and porcine reproductive and respiratory syndrome (PRRS) viruses and seroconversion in pigs fed diets containing spray-dried animal plasma. J Anim Sci 2005, 83: 1933-1938.
Pujols J, Rosell R, Russell L, Campbell J, Crenshaw J, Weaver E, Rodríguez C, Ródenas J, Polo J: Inactivation of Swine Vesicular Disease Virus in Porcine Plasma By Spray-Drying. In Proceeding of the American Association of Swine Veterinarians. FL. AASV. Perry, IA, Orlando; 2007:283.
Gerber PF, Xiao C-T, Chen Q, Zhang J, Halbur PG, Opriessnig T: The spray-drying process is sufficient to inactivate infectious porcine epidemic diarrhea virus in plasma. Vet Microbiol 2014, 174:86-92. http://dx.doi.org/10.1016/j.vetmic.2014.09.008 Gerber PF, Xiao C-T, Chen Q, Zhang J, Halbur PG, Opriessnig T: The spray-drying process is sufficient to inactivate infectious porcine epidemic diarrhea virus in plasma.Vet Microbiol 2014,174:86-92.
Pujols J, Segalés J: Survivability of porcine epidemic diarrhea virus (PEDV) in bovine plasma submitted to spray drying processing and held at different time by temperature storage conditions. Vet Microbiol 2014, 174: 427-432. http://dx.doi.org/10.1016/j.vetmic.2014.10.021 10.1016/j.vetmic.2014.10.021
Pujols J, López-Soria S, Segalés J, Fort M, Sibila M, Rosell R, Solanes D, Russell L, Campbell J, Crenshaw J, Weaver E, Polo J: Lack of transmission of porcine circovirus type 2 to weanling pigs by feeding them spray dried porcine plasma. Vet Record 2008, 163: 536-538. 10.1136/vr.163.18.536
Pujols J, Lorca-Oró C, Díaz I, Russell LE, Campbell JM, Crenshaw JD, Polo J, Mateu E, Segalés J: Commercial spray-dried porcine plasma does not transmit porcine circovirus type 2 in weaned pigs challenged with porcine reproductive and respiratory syndrome virus. Vet J 2011, 190: e16-e20. doi:10.1016/j.tvjl.2011.02.021 10.1016/j.tvjl.2011.02.021
Shen HG, Schalk S, Halbur PG, Campbell JM, Russell LE, Opriessnig T: Commercially produced spray dried porcine plasma contains high levels of porcine circovirus type 2 (PCV2) DNA but did not transmit PCV2 when fed to naïve pigs. J Anim Sci 2011, 89: 1930-1938. 10.2527/jas.2010-3502
Cal K, Sollohub K: Spray-drying technique. I: hardware and process parameters. J Pharm Sci 2010, 99: 575-586.
Sollohub K, Cal K: Spray-drying technique: II. Current applications in pharmaceutical technology. J Pharm Sci 2010, 99: 587-597.
Wang J, Mauser A, Chao SF, Remington K, Treckmann R, Kaiser K, Pifat D, Hotta J: Virus inactivation and protein recovery in a novel ultraviolet-C reactor. Vox Sang 2004, 86: 230-238. 10.1111/j.0042-9007.2004.00485.x
Peralta B, Mateu E, Casas M, De Deus N, Martín M, Pina S: Genetic characterization of the complete coding regions of genotype 3 hepatitis E virus isolated from Spanish swine herds. Virus Res 2009,139(1):111-116. 10.1016/j.virusres.2008.09.008