isomiRex: Web‐based identification of microRNAs, isomiR variations and differential expression using next‐generation sequencing datasets

FEBS Letters - Tập 587 - Trang 2629-2634 - 2013
Gaurav Sablok1, Ivan Milev2, Georgi Minkov2, Ivan Minkov2, Claudio Varotto1, Galina Yahubyan2, Vesselin Baev2
1Department of Biodiversity and Molecular Ecology, Research and Innovation Centre, Fondazione Edmund Mach, Via E Mach 1, 38010S Michele all’Adige, TN, Italy
2Department of Plant Physiology and Molecular Biology, Bioinformatics Group, University of Plovdiv, Tzar Assen 24, Plovdiv, Bulgaria

Tóm tắt

We present an open‐access web platform isomiRex, to identify isomiRs and on the fly graphical visualization of the differentially expressed miRNAs in control as well as treated library. The open‐access web‐platform is not restricted only to NGS sequence dataset from animals and potentially analyzes a wider dataset for plants, animals and viral NGS dataset supporting miRBase (version 19 supporting 193 species). The platform can handle the bloated amount of the read counts and reports the annotated microRNAs from plant, animal and viral NGS datasets. isomiRex also provides an estimation of the the isomiRs, of miRNAs with higher copy number relative to their mature reference sequences indexed in miRBase (version 19 supporting 193 species). Visually enhanced graphs potentially display differentially expressed isomiRs, which will help the user to demonstrate and correlate the abundance of the isomiR as a signature event to the specific condition. An additional module for estimating the differential expression has been implemented allowing the users to postulate the differential expression across the user input samples. The developed web‐platform can be accessed at http://bioinfo1.uni‐plovdiv.bg/isomiRex/.

Tài liệu tham khảo

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Thông tin xuất bản

FEBS Letters

Tập 587

2629-2634

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