Cleavage of Scarecrow-like mRNA Targets Directed by a Class of Arabidopsis miRNA

American Association for the Advancement of Science (AAAS) - Tập 297 Số 5589 - Trang 2053-2056 - 2002
César Llave1, Zhixin Xie1, Kristin D. Kasschau1, James C. Carrington1
1Center for Gene Research and Biotechnology and Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331 USA

Tóm tắt

Micro-RNAs (miRNAs) are regulatory molecules that mediate effects by interacting with messenger RNA (mRNA) targets. Here we show that Arabidopsis thaliana miRNA 39 (also known as miR171), a 21-ribonucleotide species that accumulates predominantly in inflorescence tissues, is produced from an intergenic region in chromosome III and functionally interacts with mRNA targets encoding several members of the Scarecrow-like ( SCL ) family of putative transcription factors. miRNA 39 is complementary to an internal region of three SCL mRNAs. The interaction results in specific cleavage of target mRNA within the region of complementarity, indicating that this class of miRNA functions like small interfering RNA associated with RNA silencing to guide sequence-specific cleavage in a developmentally controlled manner.

Từ khóa


Tài liệu tham khảo

10.1038/35053110

10.1126/science.1062961

10.1101/gad.927801

10.1126/science.1064921

10.1126/science.1065062

10.1126/science.1065329

10.1016/0092-8674(93)90529-Y

10.1016/0092-8674(93)90530-4

10.1016/S0092-8674(00)81906-6

10.1038/35002607

10.1016/S1097-2765(00)80245-2

10.1105/tpc.003210

10.1101/gad.1004402

10.1016/S0092-8674(00)80115-4

10.1105/tpc.10.2.155

Bolle C., Koncz C., Chua N. H., Genes Dev. 14, 1269 (2000).

10.1016/S0092-8674(00)80865-X

Materials and methods are available as supporting material on Science Online.

10.1126/science.286.5441.950

10.1101/gad.862301

10.1038/35005107

10.1126/science.1064023

C. Llave J. C. Carrington unpublished data.

10.1126/science.1066315

10.1017/S1355838202024032

10.1126/science.1073827

; published online 1 August 2002 (10.1126/science.1073827).

10.1101/gad.974702

10.1038/ng865

We thank L. Johansen M. Rector and B. Shaffer for valuable comments and technical support. We are also grateful to J. Valkonen and J. Kreuze for helpful suggestions on 5′-RACE procedures and D. Olszykfor providing rice tissue. C.L. was the recipient of a postdoctoral fellowship from the Ministerio de Educación y Cultura (Spain). This work was supported by grant MCB-0209836 from the National Science Foundation grants AI27832 and AI43288 from NIH and grant NRI 2002-35319-11560 from the U.S. Department of Agriculture.

Thông tin
Thông tin xuất bản

American Association for the Advancement of Science (AAAS)

Tập 297 Số 5589

2053-2056

Thông tin tác giả