Signaling pathway of ginsenoside‐Rg1 leading to nitric oxide production in endothelial cells

FEBS Letters - Tập 580 - Trang 3211-3216 - 2006
Kar Wah Leung1, Yuen-Kit Cheng2, Nai Ki Mak1, Kelvin K.C. Chan3, T.P. David Fan4, Ricky N.S. Wong1,3
1Department of Biology, Hong Kong Baptist University, Hong Kong
2Department of Chemistry, Hong Kong Baptist University, Hong Kong
3Research and Development Division, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong
4Angiogenesis and TCM Laboratory, Department of Pharmacology, University of Cambridge, Cambridge, UK

Tóm tắt

We here provide definitive evidence that ginsenoside‐Rg1, the pharmacologically active component of ginseng, is a functional ligand of the glucocorticoid receptor (GR) as determined by fluorescence polarization assay. Rg1 increased the phosphorylation of GR, phosphatidylinositol‐3 kinase (PI3K), Akt/PKB and endothelial nitric oxide synthase (eNOS) leading to increase nitric oxide (NO) production in human umbilical vein endothelial cell. Rg1‐induced eNOS phosphorylation and NO production were significantly reduced by RU486, LY294,002, or SH‐6. Also, knockdown of GR completely eliminated the Rg1‐induced NO production. This study revealed that Rg1 can indeed serve as an agonist ligand for GR and the activated GR can induce rapid NO production from eNOS via the non‐transcriptional PI3K/Akt pathway.

Tài liệu tham khảo

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FEBS Letters

Tập 580

3211-3216

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