Occurrence of tetrodotoxin in the gastropodsRapana rapiformis andR. venosa venosa

Marine Biology - Tập 111 - Trang 65-69 - 1991
D. F. Hwang1, S. C. Lu1, S. S. Jeng1
1Department of Marine Food Science, National Taiwan Ocean University, Keelung, Taiwan, Republic of China

Tóm tắt

Paralytic toxicity was detected in four of 17 specimens and six of 28 specimens of the gastropodsRapana rapiformis andR. venosa venosa, respectively, collected from Chiching (Kaohsiung City) and Nanfangao (Ilan County), Taiwan, in 1988 and 1989. The highest toxicities, calculated as tetrodotoxin (TTX) content, were 140 and 13 mouse units (MU) g−1 digestive gland inR. rapiformis andR. venosa venosa, respectively. The toxins obtained from each species were purified by ultrafiltration and Bio-Gel P-2 column chromatography. The toxin's specific toxicities (as TTX) were 56 MU mg−1 (R. rapiformis) and 52 MU mg−1 (R. venosa venosa). Results of analyses by thin-layer chromatography, cellulose acetate membrane electrophoresis and high-performance liquid chromatography showed that TTX and anhydrotetrodotoxin were responsible for the toxicity. The alkali hydrolysate of each toxin showed maximum absorption at ca. 274 nm, which is the unique absorption for the C9-base of TTX and its related substances (such as anhydrotetrodotoxin, 4-epitetrodotoxin, etc.). Here, we report for the first time the occurrence of TTX in the gastropodsR. rapiformis andR. venosa venosa.

Tài liệu tham khảo

Endo, A., Khora, S. S., Murata. M., Naoki, H., Yasumoto, T. (1988). Isolation of 11-nor TTX-6 (R)-01 and other tetrodotoxin derivatives from the pufferFugu niphoble. Tetrahedron Lett. 29: 4127–4128 Goto, T., Kishi, Y., Takahashi, S., Hinata, Y. (1965). Tetrodotoxin. Tetrahedron 21: 2059–2088 Hwang, D. F., Arakawa, O., Saito, T., Noguchi, T., Simidu, U., Tsukamoto, K., Shida, Y., Hashimoto, K. (1989). Tetrodotoxinproducing bacteria from the blue-ringed octopusOctopus maculosus. Mar. Biol. 100: 327–332 Hwang, D. F., Chech, C. H., Jeng, S. S. (1990). Occurrence of tetrodotoxin in the gastropod molluskNatica lineata (lined moon shell). Toxicon 28: 21–27 Hwang, D. F., Chueh, C. H., Tai, K. P., Lin, L. C., Jeng, S. S. (1991). Seasonal and regional variations of toxicity in the lined moon shellNatica lineata. J. Fish. Soc. Taiwan 18: 25–31 Hwang, D. F., Noguchi, T., Arakawa, O., Abe, T., Hashimoto, K. (1988). Toxicological studies on several species of puffer in Taiwan. Bull. Jap. Soc. scient. Fish. 54: 2001–2008 Hwang, D. F., Noguchi, T., Nagashima, Y., Liao, I. C., Hashimoto, K. (1987). Occurrence of paralytic shellfish poison in the purple clamSoletellina diphos (bivalve). Bull. Jap. Soc. scient. Fish. 53: 623–628 Jeon, J. K., Narita, H., Nara, M., Noguchi, T., Maruyama, J., Hashimoto, K. (1984). Occurrence of tetrodotoxin in a gastropod mullusk “arareigai”Niotha clathrata. Bull. Jap. Soc. scient. Fish. 50: 2099–2101 Kawabata, T. (1978). Food hygiene examination manual, Vol. 2, Assay method for tetrodotoxin. Tokyo, Japan Food Hygiene Association, Tokyo, p. 232–240 Maruyama, J., Noguchi, T., Jeon, J. K., Harada, T., Hashimoto, K. (1984). Occurrence of tetrodotoxin in the starfishAstropecten latespinosus. Experientia 40: 1395–1396 Maruyama, J., Noguchi, T., Narita, H., Nara, M., Jeon, J. K., Otsuka, M., Hashimoto, K. (1985). Occurrence of tetrodotoxin in a starfish,Astropecten scoparius. Agric. biol. Chem. 49: 3069–3070 Nagashima, Y., Maruyama, J., Noguchi, T., Hashimoto, K. (1987). Analysis of paralytic shellfish poison and tetrodotoxin by ionpairing high performance liquid chromatography. Bull. Jap. Soc. scient. Fish. 53: 819–823 Narita, H., Masumoto, H., Mizuno, S., Noguchi, T., Saito, T., Shida, Y., Hashimoto, K. (1989). Anatomical distribution of tetrodotoxin in a trumpet shell,Charonia sauliae, along with a comparison of toxin compositions in different tissues. J. Fd hygienic Soc. Japan 30: 522–525 Narita, H., Noguchi, T., Maruyama, J., Nara, M., Hashimoto, K. (1984). Occurrence of a tetrodotoxin-associated substance in a gastropod, “hanamushirogai”Zeuxis siquijorensis. Bull. Jap. Soc. scient. Fish. 50: 85–88 Narita, H., Noguchi, T., Maruyama, J., Ueda, Y., Hashimoto, K., Watanabe, Y., Hida, K. (1981). Occurrence of tetrodotoxin in a trumpet shell, “boshubora”Charonia sauliae. Bull. Jap. Soc. scient. Fish. 47: 935–941 Noguchi, T., Hwang, D. F., Arakawa, O., Sugita, H., Deguchi, Y., Shida, Y., Hashimoto, K. (1987).Vibrio alginolyticus, a tetrodotoxin-producing bacterium, in the intestines of the fishFugu vermicularis vermicularis. Mar. Biol. 94: 625–630 Noguchi, T., Maruyama, J., Narita, H., Hashimoto, K. (1984). Occurrence of tetrodotoxin in the gastropod molluskTutufa lissostoma (frog shell). Toxicon 22: 219–226 Noguchi, T., Maruyama, J., Ueda, J., Hashimoto, K., Harada, T. (1981). Occurrence of tetrodotoxin in the Japanese ivory shellBabylonia japonica. Bull. Jap. Soc. scient. Fish. 47: 909–913 Simidu, U., Noguchi, T., Hwang, D. F., Shida, Y., Hashimoto, K. (1987). Marine bacteria which produce tetrodotoxin. Appl. envirl Microbiol. 53: 1714–1715 Weber, M. S. (1928). The determination of the quanidine bases in urine. J. biol. Chem. 78: 465–473 Yasumoto, T., Oshima, Y., Hosaka, M., Miyakoshi, S. (1981). Occurrence of tetrodotoxin in the ivory shellBabylonia japonica from Wakasa bay. Bull. Jap. Soc. scient. Fish. 47: 929–934 Yasumoto, T., Yotsu, M., Murata, M., Naoki, H. (1988). New tetrodotoxin analogues from the newtCynops ensicauda. J. Am. chem. Soc. 110: 2344–2345