High‐throughput micro plate assays for screening flavonoid content and DPPH‐scavenging activity in sorghum bran and flour

Journal of the Science of Food and Agriculture - Tập 92 Số 11 - Trang 2326-2331 - 2012
Thomas J. Herald1,2,3, Priyadarshini Gadgil1,2,3, Michael Tilley1,2,3
1Names are necessary to report factually on available data
2USDA-ARS, Center for Grain and Animal Health Research, 1515 College Avenue, Manhattan, KS 66502, USA
3however, the USDA neither guarantees nor warrants the standard of the product, and use of the name by the USDA implies no approval of the product to the exclusion of others that may also be suitable. The USDA is an equal opportunity provider and employer.

Tóm tắt

AbstractBACKGROUND: Sorghum possesses phenolic compounds that are health‐promoting constituents of the grain. There are approximately 40 000 sorghum accessions, many of which have not been evaluated for the grain's health‐promoting potential. Conventional methods for measuring total phenolic content, flavonoid content and 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH)‐scavenging capacity are time‐consuming and labour‐intensive, resulting in low overall throughput. The objective of this study was to develop a high‐throughput screening assay for large sorghum sample sets to determine flavonoid and phenolic content and to modify existing DPPH and total phenolic assays.RESULTS: The 96‐well assays exhibited a correlation of > 0.9 with the conventional assays. The 96‐well assays allowed for up to 64 samples to be run per day compared with 20–24 samples (depending on the test) for the conventional methods. The 96‐well assays had excellent accuracy (97.65–106.16% recovery), precision (1.06–8.28% coefficient of variation (CV)) and reproducibility (1.32–2.13% CV inter‐day and 1.36–2.09% CV intra‐day).CONCLUSION: The high‐throughput 96‐well plate method proved to be as robust and reproducible as the conventional method for determining total phenolic content, flavonoid content and DPPH‐scavenging capacity in either sorghum bran or flour. Published 2012 by John Wiley & Sons, Ltd.

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Thông tin xuất bản

Journal of the Science of Food and Agriculture

Tập 92 Số 11

2326-2331

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