Significance of antibody to hepatitis B core antigen in blood donors as determined by their serologic response to hepatitis B vaccine

Transfusion - Tập 33 Số 5 - Trang 362-367 - 1993
SK Aoki1, D J Finegold2, I K Kuramoto3, C. Douville4, Cynthia A. Richards5, R. Randell6, Leonor P. Fernando7, Paul V. Holland8, Jerome B. Zeldis9
1Sacramento Medical Foundation Blood Center, California.
2Don Finegold, MD, Fellow, Department of Gastroenterology, University of California, Sacramento, CA.
3Isamu K. Kuramoto, BS, Research Coordinator, Center for Blood Research, Sacramento Medical Foundation.
4Claire Douville, BS, MT(ASCP), Research Associate, Center for Blood Research.
5Cheryl Richards, BS, MT(ASCP), Technical Director, Center for Blood Research.
6Robert Randell, MD, Director, Center for Blood Research.
7Leonor Fernando, MD, Associate Medical Director, Sacramento Medical Foundation.
8Paul V. Holland, MD, Medical Director and CEO, Sacramento Medical Foundation.
9Jerome B. Zeldis, MD, PhD, Department of Gastroenterology, University of California, Sacramento, CA.

Tóm tắt

Because large numbers of volunteer blood donors may be disqualified for “false‐positive” results on tests for antibody to hepatitis B core antigen (anti‐HBc), a more specific definition of anti‐HBc enzyme immunoassay (EIA)‐reactive was evaluated, including only those donor samples that were “strongly” reactive (sample‐to‐cutoff absorbance ratio, < 0.45). Results using this definition and other anti‐HBc test methods were compared to the serologic response (antibody to hepatitis B surface antigen [anti‐HBsAg]) to hepatitis B vaccination. Fifty‐eight volunteer blood donors who had previously been deferred as donors, because of reactive anti‐HBc tests (all other blood screening tests were negative, including those for HBsAg and anti‐HBsAg) on two occasions, were vaccinated for hepatitis B. It was assumed that an anamnestic response to vaccine indicated past infection with hepatitis B, while a primary response to vaccine indicated lack of past infection. One (2%) of 43 donors with a historically “weak” anti‐HBc (reactive absorbance ratio, > or = 0.45) had an anamnestic response to vaccine, compared to 8 (53%) of 15 with historically “strong” anti‐HBc (reactive absorbance ratio, < 0.45) (p < 0.005). Anti‐HBc testing using the microparticle EIA method also correlated well with hepatitis B vaccination results. The use of a narrower definition of “reactive” for anti‐HBc EIA testing yielded much more specific, but slightly less sensitive, results.

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Transfusion

Tập 33 Số 5

362-367

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