Lutropin-Choriogonadotropin Receptor: An Unusual Member of the G Protein-Coupled Receptor Family

American Association for the Advancement of Science (AAAS) - Tập 245 Số 4917 - Trang 494-499 - 1989
Keith McFarland1, Rolf Sprengel2, Heidi Phillips1, Martin Köhler2, Nora Rosemblit3, K Nikolics1, Deborah L. Segaloff3, Peter H. Seeburg2
1Department of Developmental Biology, Genetech, Inc., South San Francisco, CA 94080.
2Zentrum for Molekulare Biologie Heidelberg, University of Heidelberg, F.R.G.
3The Population Council, New York, NY 10021.

Tóm tắt

A complementary DNA (cDNA) for the rat luteal lutropin-choriogonadotropin receptor (LH-CG-R) was isolated with the use of a DNA probe generated in a polymerase chain reaction with oligonucleotide primers based on peptide sequences of purified receptor protein. As would be predicted from the cDNA sequence, the LH-CG-R consists of a 26-residue signal peptide, a 341-residue extracellular domain displaying an internal repeat structure characteristic of members of the leucine-rich glycoprotein (LRG) family, and a 333-residue region containing seven transmembrane segments. This membrane-spanning region displays sequence similarity with all members of the G protein-coupled receptor family. Hence, the LH-CG-R gene may have evolved by recombination of LRG and G protein-coupled receptor genes. Cells engineered to express LH-CG-R cDNA bind human choriogonadotropin with high affinity and show an increase in cyclic adenosine monophosphate when exposed to hormone. As revealed by RNA blot analysis and in situ hybridization, the 4.4-kilobase cognate messenger RNA is prominently localized in the rat ovary.

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American Association for the Advancement of Science (AAAS)

Tập 245 Số 4917

494-499

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