MicroRNA expression in the adult mouse central nervous system

RNA - Tập 14 Số 3 - Trang 432-444 - 2008
Mads Bak1, Asli Silahtaroglu1, Morten Møller2, Mette Damkjær Bartels1, Martin F. Rath2, Boris V. Skryabin3, Niels Tommerup1, Sakari Kauppinen4,1
1Wilhelm Johannsen Center for Functional Genome Research, Department of Cellular and Molecular Medicine, University of Copenhagen, DK-2200 Copenhagen N, Denmark.
2Department of Neuroscience and Pharmacology, Panum Institute, University of Copenhagen, DK-2200 Copenhagen N, Denmark
3Institute of Experimental Pathology, ZMBE, University of Mu ¨nster, D-48149 Mu ¨nster, Germany
4Santaris Pharma, DK-2970 Hoersholm, Denmark

Tóm tắt

microRNAs are ∼22 nucleotide endogenous noncoding RNAs that post-transcriptionally repress expression of protein-coding genes by base-pairing with the 3′-untranslated regions of the target mRNAs. We present here an inventory of miRNA expression profiles from 13 neuroanatomically distinct areas of the adult mouse central nervous system (CNS). Microarray profiling in combination with real-time RT-PCR and LNA (locked nucleic acid)-based in situ hybridization uncovered 44 miRNAs displaying more than threefold enrichment in the spinal cord, cerebellum, medulla oblongata, pons, hypothalamus, hippocampus, neocortex, olfactory bulb, eye, and pituitary gland. These findings suggest that a large number of mouse CNS-expressed miRNAs may be associated with specific functions within these regions. Notably, more than 50% of the identified mouse CNS-enriched miRNAs showed different expression patterns compared to those reported in zebrafish, although the mature miRNA sequences are nearly 100% conserved between the two vertebrate species. The inventory of miRNA profiles in the adult mouse CNS presented here provides an important step toward further elucidation of miRNA function and miRNA-related gene regulatory networks in the mammalian central nervous system.

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RNA

Tập 14 Số 3

432-444

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