Hao Wu1, Ana C. D’Alessio2, Shinsuke Ito2, Zhibin Wang3, Kairong Cui4, Keji Zhao4, Yi Eve Sun5, Yi Zhang2
1Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California 90095, USA.
2University of North Carolina at Chapel Hill
3Bloomberg School of Public Health
4NATIONAL INSTITUTES OF HEALTH
5University of California at, Los Angeles
Tóm tắt
Recent studies have demonstrated that the Ten-eleven translocation (Tet) family proteins can enzymatically convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). While 5mC has been studied extensively, little is known about the distribution and function of 5hmC. Here we present a genome-wide profile of 5hmC in mouse embryonic stem (ES) cells. A combined analysis of global 5hmC distribution and gene expression profile in wild-type and Tet1-depleted ES cells suggests that 5hmC is enriched at both gene bodies of actively transcribed genes and extended promoter regions of Polycomb-repressed developmental regulators. Thus, our study reveals the first genome-wide 5hmC distribution in pluripotent stem cells, and supports its dual function in regulating gene expression.
