Introgression molecular analysis of a leaf rust resistance gene from Coffea liberica into C. arabica L.
Tóm tắt
Leaf rust caused by the fungus Hemileia vastatrix is the most devastating disease of arabica coffee (Coffea arabica). Therefore, developing leaf rust-resistant varieties has been a breeding objective of the highest priority in many countries. The purpose of the present work was to gain insight into the mechanism of introgression into C. arabica of a leaf rust resistance gene from C. liberica (i.e. SH3 resistance factor) and to identify associated molecular markers. An F2 progeny (i.e. 101 individuals) derived from a cross between Matari, an arabica accession and liberica-introgressed line S.288, was evaluated for resistance against three different races of H. vastatrix. The progeny segregated for the SH3 gene in a 3:1 ratio, as expected for a single dominant gene. Amplified fragment length polymorphism analysis of a population subset using 80 different primer combinations revealed that at least half of the total polymorphism observed in the population is associated with introgression of C. liberica chromosome fragments. Furthermore, 15 primer combinations generating candidate marker bands associated with the SH3 resistance gene were used to analyse the whole F2 population. A total of 34 marker bands originating from S.288 and attributable to introgression were scored. None exhibited segregation distortion. Linkage analysis revealed only three distinct introgressed fragments corresponding to a total length of 52.8 cM. Twenty-one markers were strongly associated (LOD score >14) with the SH3 gene and were grouped together in a single linkage group of 6.3 cM. The results are discussed in relation to the efficient use of genetic resources in arabica breeding.
Tài liệu tham khảo
Anonymous (2000) Coffee guide. Coffee Board, Bangalore
Anthony F, Combes MC, Astorga C, Bertrand B, Graziosi G, Lashermes P (2002) The origin of cultivated Coffea arabica L. varieties revealed by AFLP and SSR markers. Theor Appl Genet 104:894–900
Arru L, Faccini N, Govoni C, Cattivelli L, Pecchioni N, Delogu G, Stanca AM, Vale G (2003) The PCR-based marker MWG2018 linked to the RDG2A leaf stripe resistance gene is a useful tool for assessing barley resistance in breeding programs. Crop Sci 43:1036–1042
Bettencourt AJ, Rodrigues CJ Jr (1988) Principles and practice of coffee breeding for resistance to rust and other disease. In: Clarke RJ, Macrae R (eds) Coffee, vol 4. Agronomy. Elsevier, London, pp 199–234
D’Oliveira B (1957) As ferrugens do cafeeiro. Rev Café Port 4:5–15
Duvick DN (1996) Plant breeding, an evolutionary concept. Crop Sci 36:539–548
Eskes AB, Toma-Braghini M (1982) Assessment methods for resistance to coffee leaf rust (Hemileia vastatrix Berk. and Br.). Plant Prot Bull 29:56–66
Frisch M, Melchinger AE (2001) The length of the intact donor chromosome segment around a target gene in marker-assisted backcrossing. Genetics 157:1343–1356
Goff SA, Ricke D, Lan TH, Presting G, Wang R, Dunn M, Glazebrook J, Sessions A, Oeller P, Varma H, Hadley D, Hutchison D, Martin C, Katagiri F, Lange BM, Moughamer T, Xia Y, Budworth P, Zhong J, Miguel T, Paszkowski U, Zhang S, Colbert M, Sun WL, Chen L, Cooper B, Park S, Wood TC, Mao L, Quail P, Wing R, Dean R, Yu Y, Zharkikh A, Shen R, Sahasrabudhe S, Thomas A, Cannings R, Gutin A, Pruss D, Reid J, Tavtigian S, Mitchell J, Eldredge G, Scholl T, Miller RM, Bhatnagar S, Adey N, Rubano T, Tusneem N, Robinson R, Feldhaus J, Macalma T, Oliphant A, Briggs S (2002) A draft sequence of the rice genome (Oryza sativa L. ssp. japonica). Science 296:92–100
Herrera JC, Combes MC, Anthony F, Charrier A, Lashermes P (2002) Introgression into the allotetraploid coffee (Coffea arabica L.): segregation and recombination of the C. canephora genome in the tetraploid interspecific hybrid (C. arabica × C. canephora). Theor Appl Genet 104:661–668
Hospital F (2001) Size of donor chromosome segments around introgressed loci and reduction of linkage drag in marker-assisted backcross programs. Genetics 158:1363–1379
Koebner RMD, Kirsch F, Torpe C, Prins R (1998) AFLPs as a source of STS markers in alien introgression. In: Slinkard AE (ed) Proc 9th Int Wheat Genet Symp, vol 1. University Extension Press, Saskatoon, pp 118–122
Kosambi DD (1944) The estimates of map distances from recombination values. Ann Eugen 12:172–175
Kukhang TD, Mawardi S, Eskes AB (1993) Studies on the inheritance of the SH3 resistance factor to coffee leaf rust. In: ASIC (ed) Proc 15th Assoc Sci Int Café (ASIC) Colloq. ASIC, Paris, pp 776–778
Kushalappa AC, Eskes AB (1989) Advances in coffee rust research. Annu Rev Phytopathol 27:503–531
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Lashermes P, Combes MC, Robert J, Trouslot P, D’Hont A, Anthony F, Charrier A (1999) Molecular characterisation and origin of the Coffea arabica L. genome. Mol Gen Genet 261:259–266
Lashermes P, Combes MC, Topart P, Graziosi G, Bertrand B, Anthony F (2000a) Molecular breeding in coffee (Coffea arabica L.) In: Sera R, Soccol S, Pandey A, Roussos AR (eds) Coffee biotechnology and quality. Kluwer, Dordrecht, pp 101–112
Lashermes P, Andrzejewski S, Bertrand B, Combes MC, Dussert S, Graziosi G, Trouslot P, Anthony F (2000b) Molecular analysis of introgressive breeding in coffee (Coffea arabica L.). Theor Appl Genet 100:139–146
Liu J, Liu D, Tao W, Li W, Wang S, Chen P, Cheng S, Gao D (2000) Molecular marker-facilitated pyramiding of different genes for powdery mildew resistance in wheat. Plant Breed 119:21–24
Moreno G, Alvarado G (2000) La variedad Colombia: veinte anos de adopcion y comportalmiento frente a nuevas razas de la roya del cafeto. Cenicafe Tech Bull 22:32
Noir S, Anthony F, Bertrand B, Combes MC, Lashermes P (2003) Identification of a major gene (Mex-1) from Coffea canephora conferring resistance to Meloidogyne exigua in Coffea arabica. Plant Pathol 52:97–103
Prakash NS, Combes MC, Naveen KS, Lashermes P (2002) AFLP analysis of introgression in coffee cultivars (Coffea arabica L.) derived from a natural interspecific hybrid. Euphytica 124:265–271
Ramachandran M, Srinivasan CS (1979) Four generations of selection for resistance to race I of leaf rust in arabica cv. S.288 × ‘Kents’. Indian Coffee 43:159–161
Richly E, Kurth J, Leister D (2002) Mode of amplification and reorganization of resistance genes during recent Arabidopsis thaliana evolution. Mol Biol Evol 19:76–84
Rodrigues CJ Jr, Bettencourt AJ, Rijo L (1975) Races of the pathogen and resistance to coffee rust. Annu Rev Phytopathol 13:49–70
Rodrigues CJ Jr, Varzea VM, Godinho IL, Palma S, Rato RC (1993) New physiological races of Hemileia vastatrix. In: Proc 15th ASIC Colloquium (Montpellier). ASIC, Paris, pp 318–321
Santaram A, Sreenath HL (2000) Genetic fingerprinting of coffee genotypes with varying resistance to rust. In: Muraleedharan N, Rajkumar R (eds) Recent advances in plantation crops research. pp 57–62
Sreenivasan MS, Ram AS, Prakash NS (1994) Search for new sources of resistance to coffee rust. In: Central Coffee Research Institute (ed) Project report on “Pathology and improvement of coffee for the main diseases”, a collaborative project of CRRC, Portugal and CCRI, India. CRRC/CCRI, Portugal/India, p 24
Srinivasan KH, Narasimhaswamy RL (1975) A review of coffee breeding work done at the Government coffee experiment station, Balehonnur. Indian Coffee 34:311–321
Van der Vossen HAM (2001) Coffee breeding practices. In: Clarke RJ, Vitzthum OG (eds) Coffee recent developments. Agronomy, vol 1. Blackwell, London, pp 184–201
Vishveshwara S (1974) Periodicity of Hemileia in arabica selection. Indian Coffee 38:49–51
Vos P, Hogers R, Bleeker M, Reijans M, Van der Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414
Wagner M, Bettencourt AJ (1965) Inheritance of reaction to Hemileia vastatrix Berk & Br. in Coffea arabica L. Progress report 1960–1965, Coffee Rusts Research Center, Oeira