Synbiotic Supplementation Improves Obesity Index and Metabolic Biomarkers in Thai Obese Adults: A Randomized Clinical Trial

Foods - Tập 10 Số 7 - Trang 1580
Chaiyavat Chaiyasut1, Bhagavathi Sundaram Sivamaruthi1, Periyanaina Kesika1, Suchanat Khongtan1, Nanticha Khampithum1, Subramanian Thangaleela1, Sartjin Peerajan2, Akkarach Bumrungpert3,4, Khontaros Chaiyasut5, Sasithorn Sirilun1, Phakkharawat Sittiprapaporn6
1Innovation Center for Holistic Health, Nutraceuticals, and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, 50200, Thailand
2Health Innovation Institute, Chiangmai 50200, Thailand.
3Mahidol Nutrition Society, Faculty of Public Health, Mahidol University, Bangkok 10400, Thailand.
4Research Center of Nutraceuticals and Natural Products for Health & Anti-Aging, College of Integrative Medicine, Dhurakij Pundit University, Bangkok 10210, Thailand.
5Institute of Research and Development, Chiang Mai Rajabhat University, Chiangmai 50300, Thailand.
6Neuropsychological Research Laboratory, Department of Anti-Aging and Regenerative Science, School of Anti-Aging and Regenerative Medicine, Mae Fah Luang University, Bangkok 11120, Thailand.

Tóm tắt

The cluster of metabolic disorders includes obesity, dyslipidemia, hypertension, and glucose intolerance, increasing the risk of developing cardiovascular diseases and type 2 diabetes. Evolving proofs suggest an essential role of microbiota in human health and disease, including digestion, energy and glucose metabolism, immunomodulation, and brain function. The frequency of overweight is increasing, and the main causes for this are highly processed foods and less active lifestyles. Research is underway to unravel the probable relationship between obesity and intestinal microbiota. Here, we propose a method to understand and elucidate the synergistic function of prebiotics and probiotics in treating obesity. The biomarkers of obesity, such as cholesterol, gut permeability, oxidative stress, bacterial toxins, cytokines, and short-chain fatty acids, were analyzed in Thai obese individuals after being supplemented with a synbiotic preparation containing Lactobacillus paracasei, Bifidobacterium longum, Bifidobacterium breve, inulin, and fructooligosaccharide. The results reveal that the supplementation of synbiotics significantly altered the obesity-associated biomarkers in an appositive way. Further studies are warranted to use synbiotics as an adjuvant therapy for the management of obesity-related health issues.

Từ khóa


Tài liệu tham khảo

Sivamaruthi, 2019, A review on role of microbiome in obesity and antiobesity properties of probiotic supplements, Biomed. Res. Int., 2019, 3291367, 10.1155/2019/3291367

(2021, January 10). Obesity and Overweight. Available online: https://www.who.int/en/news-room/fact-sheets/detail/obesity-and-overweight.

Ley, 2006, Human gut microbes associated with obesity, Nature, 444, 1022, 10.1038/4441022a

Tremaroli, 2012, Functional interactions between the gut microbiota and host metabolism, Nature, 489, 242, 10.1038/nature11552

Nagpal, 2018, Obesity-linked gut microbiome dysbiosis associated with derangements in gut permeability and intestinal cellular homeostasis independent of diet, J. Diabetes Res., 2018, 3462092, 10.1155/2018/3462092

Neves, 2016, Impact of the gut microbiota on inflammation, obesity, and metabolic disease, Genome Med., 8, 1

Sivamaruthi, 2019, Probiotics in human mental health and diseases-A mini review, Trop. J. Pharm. Res., 18, 889, 10.4314/tjpr.v18i4.29

Sivamaruthi, B.S., Suganthy, N., Kesika, P., and Chaiyasut, C. (2020). The role of microbiome, dietary supplements, and probiotics in autism spectrum disorder. Int. J. Environ. Res. Public Health, 17.

Sivamaruthi, 2020, The role of probiotics in colorectal cancer management, Evid. Based Complement. Alternat. Med., 2020, 3535982, 10.1155/2020/3535982

De Vrese, M., and Schrezenmeir, A.J. (2008). Probiotics, prebiotics, and synbiotics. Food Biotechnology, Springer.

Saghaei, 2004, Random allocation software for parallel group randomized trials, BMC Med. Res. Methodol., 4, 1, 10.1186/1471-2288-4-26

Minami, 2018, Effects of Bifidobacterium breve B-3 on body fat reductions in pre-obese adults: A randomized, double-blind, placebo-controlled trial, Biosci. Microbiota Food Health., 37, 67, 10.12938/bmfh.18-001

Taoran, 2017, Validation of analytical methods for plasma total antioxidant capacity by comparing with urinary 8-isoprostane level, J. Microbiol. Biotechnol., 27, 388, 10.4014/jmb.1604.04053

Kambayashi, 2009, Efficient assay for total antioxidant capacity in human plasma using a 96-well microplate, J. Clin. Biochem. Nutr., 44, 46, 10.3164/jcbn.08-162

Zeb, 2016, A Simple Spectrophotometric Method for the Determination of Thiobarbituric Acid Reactive Substances in Fried Fast Foods, J. Anal. Methods Chem., 2016, 9412767, 10.1155/2016/9412767

Atasayar, 2004, Malondialdehyde quantification in blood plasma of tobacco smokers and non-smokers, Fabad J. Pharm. Sci., 29, 15

Joe, 1969, Superoxide Dismutase, J. Biol. Chem., 224, 6049

Rahman, 2006, Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method, Nat. Protoc., 1, 3159, 10.1038/nprot.2006.378

Kotani, 2009, Determination of short-chain fatty acids in rat and human feces by high-performance liquid chromatography with electrochemical detection, Anal. Sci., 25, 1007, 10.2116/analsci.25.1007

Torii, 2010, Measurement of short-chain fatty acids in human faeces using high-performance liquid chromatography: Specimen stability, Ann. Clin. Biochem., 47, 447, 10.1258/acb.2010.010047

Birkett, 1995, Simple high-performance liquid chromatographic analysis of phenol and p-cresol in urine and feces, J. Chromatogr. B Biomed. Sci. Appl., 674, 187, 10.1016/0378-4347(95)00324-X

Chen, 2007, Effects of raw potato starch and live weight on fat and plasma skatole, indole and androstenone levels measured by different methods in entire male pigs, Food Chem., 101, 439, 10.1016/j.foodchem.2005.11.054

Nowak, 2007, Ability of intestinal lactic bacteria to bind or/and metabolise phenol and p-cresol, Ann. Microbiol., 57, 329, 10.1007/BF03175068

Sequeira, I.R., Lentle, R.G., Kruger, M.C., and Hurst, R.D. (2014). Standardising the lactulose mannitol test of gut permeability to minimise error and promote comparability. PLoS ONE, 9.

Barengolts, 2016, Gut microbiota, prebiotics, probiotics, and synbiotics in management of obesity and prediabetes: Review of randomized controlled trials, Endocr. Pract., 22, 1224, 10.4158/EP151157.RA

Raninen, 2011, Dietary fiber type reflects physiological functionality: Comparison of grain fiber, inulin, and polydextrose, Nutr. Rev., 69, 9, 10.1111/j.1753-4887.2010.00358.x

Vallianou, 2020, Probiotics, Prebiotics, Synbiotics, Postbiotics, and Obesity: Current evidence, controversies, and perspectives, Curr. Obes. Rep., 9, 179, 10.1007/s13679-020-00379-w

Park, 2015, Probiotics for weight loss: A systematic review and meta-analysis, Nutr. Res., 35, 566, 10.1016/j.nutres.2015.05.008

Ji, 2019, Dose-dependent and strain-dependent anti-obesity effects of Lactobacillus sakei in a diet induced obese murine model, PeerJ, 7, e6651, 10.7717/peerj.6651

Ji, 2019, Amelioration of obesity-related biomarkers by Lactobacillus sakei CJLS03 in a high-fat diet-induced obese murine model, Sci. Rep., 9, 6821, 10.1038/s41598-019-43092-y

Vulevic, 2013, A mixture of trans-galactooligosaccharide reduces markers of metabolic syndrome and modulates the fecal microbiota and immune function of overweight adults, J. Nutr., 143, 324, 10.3945/jn.112.166132

Canfora, 2017, Supplementation of diet with galacto-oligosaccharides increases bifidobacteria, but not insulin sensitivity, in obese prediabetic individuals, Gastroenterology, 153, 87, 10.1053/j.gastro.2017.03.051

Butz, 2018, An attenuated Salmonella enterica Serovar Typhimurium strain and galacto-oligosaccharides accelerate clearance of salmonella infections in poultry through modifications to the gut microbiome, Appl. Environ. Microbiol., 84, e02526-17, 10.1128/AEM.02526-17

Sergeev, I.N., Aljutaily, T., Walton, G., and Huarte, E. (2020). Effects of Synbiotic Supplement on Human Gut Microbiota, Body Composition and Weight Loss in Obesity. Nutrients, 12.

Kadooka, 2010, Regulation of abdominal adiposity by probiotics (Lactobacillus gasseri SBT2055) in adults with obese tendencies in a randomized controlled trial, Eur. J. Clin. Nutr., 64, 636, 10.1038/ejcn.2010.19

Aoun, 2020, The influence of the gut microbiome on obesity in adults and the role of probiotics, prebiotics, and synbiotics for weight loss, Prev. Nutr. Food Sci., 25, 113, 10.3746/pnf.2020.25.2.113

Hadi, 2020, Effects of synbiotic consumption on lipid profile: A systematic review and meta-analysis of randomized controlled clinical trials, Eur. J. Nutr., 59, 2857, 10.1007/s00394-020-02248-7

Hadi, 2019, Efficacy of synbiotic supplementation in patients with nonalcoholic fatty liver disease: A systematic review and meta-analysis of clinical trials: Synbiotic supplementation and NAFLD, Crit. Rev. Food Sci. Nutr., 59, 2494, 10.1080/10408398.2018.1458021

Konstantinov, 2008, S layer protein A of Lactobacillus acidophilus NCFM regulates immature dendritic cell and T cell functions, Proc. Natl. Acad. Sci. USA, 105, 19474, 10.1073/pnas.0810305105

Ouwehand, 2008, Influence of a combination of Lactobacillus acidophilus NCFM and lactitol on healthy elderly: Intestinal and immune parameters, Br. J. Nutr., 101, 367, 10.1017/S0007114508003097

Kimoto, 2002, Cholesterol removal from media by Lactococci, J. Dairy Sci., 85, 3182, 10.3168/jds.S0022-0302(02)74406-8

Lye, 2010, Removal of cholesterol by lactobacilli via incorporation and conversion to coprostanol, J. Dairy Sci., 93, 1383, 10.3168/jds.2009-2574

Begley, 2006, Bile salt hydrolase activity in probiotics, Appl. Environ. Microbiol., 72, 1729, 10.1128/AEM.72.3.1729-1738.2006

Patel, 2010, Probiotic bile salt hydrolase: Current developments and perspectives, Appl. Biochem. Biotechnol., 162, 166, 10.1007/s12010-009-8738-1

Wiciński, M., Gębalski, J., Gołębiewski, J., and Malinowski, B. (2020). Probiotics for the treatment of overweight and obesity in humans-A review of clinical trials. Microorganisms, 8.

Hofmann, 2014, Probiotics: Achieving a better regulatory ft, Food Drug Law J., 69, 237

Crovesy, 2017, Effect of Lactobacillus on body weight and body fat in overweight subjects: A systematic review of randomized controlled clinical trials, Int. J. Obes., 41, 1607, 10.1038/ijo.2017.161

Krumbeck, 2018, Probiotic Bifidobacterium strains and galacto-oligosaccharides improve intestinal barrier function in obese adults but show no synergism when used together as synbiotics, Microbiome, 6, 121, 10.1186/s40168-018-0494-4

Barathikannan, K., Chelliah, R., Rubab, M., Daliri, E.B., Elahi, F., Kim, D.H., Agastian, P., Oh, S.Y., and Oh, D.H. (2019). Gut microbiome modulation based on probiotic application for anti-obesity: A review on efficacy and validation. Microorganisms, 7.

Hotamisligil, 2007, Endoplasmic reticulum stress and inflammation in obesity and type 2 diabetes, Novartis Found. Symp., 286, 86, 10.1002/9780470985571.ch8

Hotamisligil, 2008, Nutrient sensing and inflammation in metabolic diseases, Nat. Rev. Immunol., 8, 923, 10.1038/nri2449

Scarpellini, 2012, Obesity and metabolic syndrome: An inflammatory condition, Dig. Dis., 30, 148, 10.1159/000336664

Ferrarese, 2018, Probiotics, prebiotics and synbiotics for weight loss and metabolic syndrome in the microbiome era, Eur. Rev. Med. Pharmacol. Sci., 22, 7588

Dehghan, 2013, Effects of high performance inulin supplementation on glycemic status and lipid profile in women with type 2 diabetes: A randomized, placebo-controlled clinical trial, Health Promot. Perspect., 3, 55

Genta, 2009, Yacon syrup: Beneficial effects on obesity and insulin resistance in humans, Clin. Nutr., 28, 182, 10.1016/j.clnu.2009.01.013

Dehghan, 2013, Effects of high performance inulin supplementation on glycemic control and antioxidant status in women with type 2 diabetes, Diabetes Metab. J., 37, 140, 10.4093/dmj.2013.37.2.140

Dehghan, 2014, Oligofructose-enriched inulin improves some inflammatory markers and metabolic endotoxemia in women with type 2 diabetes mellitus: A randomized controlled clinical trial, Nutrition, 30, 418, 10.1016/j.nut.2013.09.005

Hotamisligil, 1995, Increased adipose tissue expression of tumor necrosis factor-alpha in human obesity and insulin Resistance, J. Clin. Investig., 95, 2409, 10.1172/JCI117936

Lubberts, 2008, I/-/H targeting: On the road to prevent chronic destructive arthritis?, Cytokine, 41, 84, 10.1016/j.cyto.2007.09.014

Pang, 1994, GM-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8, IL-10, ICAM-1 and VCAM-1 gene expression and cytokine production in human duodenal fibroblasts stimulated with lipopolysaccharide, IL-l alpha and TNF- alpha, Clin. Exp. Immunol., 96, 437, 10.1111/j.1365-2249.1994.tb06048.x

Afarideh, 2015, Association of Peripheral 5-Hydroxyindole-3-Acetic Acid, A serotonin derivative, with metabolic syndrome and low-grade inflammation, Endocr Pract., 21, 711, 10.4158/EP14442.OR

Tecott, 1995, Eating disorder and epilepsy in mice lacking 5-HT2c serotonin receptors, Nature, 374, 542, 10.1038/374542a0

Jonnakuty, 2008, What do we know about serotonin?, J. Cell Physiol., 217, 301, 10.1002/jcp.21533

Kinoshita, 2010, Regulation of adipocyte differentiation by activation of serotonin (5-HT) receptors 5-HT2AR and 5-HT2CR and involvement of microRNA-448-mediated repression of KLF5, Mol. Endocrinol., 24, 1978, 10.1210/me.2010-0054

Kim, 2011, Metabolomic analysis of livers and serum from high-fat diet induced obese mice, J. Proteome Res., 10, 722, 10.1021/pr100892r

Wang, 2000, Human zonulin, a potential modulator of intestinal tight junctions, J. Cell Sci., 113, 4435, 10.1242/jcs.113.24.4435

Sapone, 2006, Zonulin upregulation is associated with increased gut permeability in subjects with type 1 diabetes and their relatives, Diabetes, 55, 1443, 10.2337/db05-1593

Tripathi, 2009, Identification of human zonulin, a physiological modulator of tight junctions, as prehaptoglobin-2, Proc. Natl. Acad. Sci. USA, 106, 16799, 10.1073/pnas.0906773106

Aasbrenn, M., Lydersen, S., and Farup, P.G. (2020). Changes in serum zonulin in individuals with morbid obesity after weight-loss interventions: A prospective cohort study. BMC Endocr Disord., 20.

Sadeghian, 2020, The effects of probiotic/synbiotic on serum level of zonulin as a biomarker of intestinal permeability: A systematic review and meta-analysis, Iran. J. Public Health, 49, 1222

Kamari, 2011, Lack of interleukin-1α or interleukin-1β inhibits transformation of steatosis to steatohepatitis and liver fibrosis in hypercholesterolemic mice, J. Hepatol., 55, 1086, 10.1016/j.jhep.2011.01.048

Nov, O., Shapiro, H., Ovadia, H., Tarnovscki, T., Dvir, I., Shemesh, E., Kovsan, J., Shelef, I., Carmi, Y., and Voronov, E. (2013). Interleukin-1β regulates fat-liver crosstalk in obesity by auto-paracrine modulation of adipose tissue inflammation and expandability. PLoS ONE, 8.