The UDP-glucosyltransferase multigene family in Bombyx mori

Springer Science and Business Media LLC - 2008
Feifei Huang1, Chun-Li Chai1, Ze Zhang2,1, Liu Zeng-hu1, Fangyin Dai1, Cheng Lü1, Xiang Zhang1
1The Key Sericultural Laboratory of Agricultural Ministry, Institute of Sericulture and Systems Biology, Southwest University, Chongqing, PR China
2The Institute of Agricultural and Life Sciences, Chongqing University, Chongqing, PR China

Tóm tắt

Abstract Background

Glucosidation plays a major role in the inactivation and excretion of a great variety of both endogenous and exogenous compounds. A class of UDP-glycosyltransferases (UGTs) is involved in this process. Insect UGTs play important roles in several processes, including detoxication of substrates such as plant allelochemicals, cuticle formation, pigmentation, and olfaction. Identification and characterization of Bombyx mori UGT genes could provide valuable basic information for this important family and explain the detoxication mechanism and other processes in insects.

 Results

Taking advantage of the newly assembled genome sequence, we performed a genome-wide analysis of the candidate UGT family in the silkworm, B. mori. Based on UGT signature and their similarity to UGT homologs from other organisms, we identified 42 putative silkworm UGT genes. Most of them are clustered on the silkworm chromosomes, with two major clusters on chromosomes 7 and 28, respectively. The phylogenetic analysis of these identified 42 UGT protein sequences revealed five major groups. A comparison of the silkworm UGTs with homologs from other sequenced insect genomes indicated that some UGTs are silkworm-specific genes. The expression patterns of these candidate genes were investigated with known expressed sequence tags (ESTs), microarray data, and RT-PCR method. In total, 36 genes were expressed in tissues examined and showed different patterns of expression profile, indicating that these UGT genes might have different functions.

 Conclusion

B. mori possesses a largest insect UGT gene family characterized to date, including 42 genes. Phylogenetic analysis, genomic organization and expression profiles provide an overview for the silkworm UGTs and facilitate their functional studies in future.

Từ khóa


Tài liệu tham khảo

Tephly TR, Burchell B: UDP-glucuronosyltransferases: a family of detoxifying enzymes. Trends Pharmacol Sci. 1990, 11: 276-279. 10.1016/0165-6147(90)90008-V.

Brierley CH, Burchell B: Human UDP-glucuronosyl transferases: chemical defence, jaundice and gene therapy. Bioessays. 1993, 15: 749-754. 10.1002/bies.950151108.

Meyer UA: Overview of enzymes of drug metabolism. J Pharmacokinet Biopharm. 1996, 24: 449-459. 10.1007/BF02353473.

Nebert DW, Gonzalez FJ: P450 genes: structure, evolution, and regulation. Annu Rev Biochem. 1987, 56: 945-993. 10.1146/annurev.bi.56.070187.004501.

Guengerich FP: Catalytic selectivity of human cytochrome P450 enzymes: relevance to drug metabolism and toxicity. Toxicol Lett. 1994, 70 (2): 133-138. 10.1016/0378-4274(94)90156-2.

Maser E, Opperman UC: Role of type-1 11beta-hydroxysteroid dehydrogenase in detoxification processes. Eur J Biochem. 1997, 249: 365-369. 10.1111/j.1432-1033.1997.00365.x.

Shen H, Kauvar L, Tew KD: Importance of glutathione and associated enzymes in drug response. Oncol Res. 1997, 9: 295-302.

Feyereisen R: Molecular biology of insecticide resistance. Toxicol Lett. 1995, 82: 83-90. 10.1016/0378-4274(95)03470-6.

Burchell B, Coughtrie MW: UDP-glucuronosyltransferases. Pharmacol Ther. 1989, 43: 261-289. 10.1016/0163-7258(89)90122-8.

Mackenzie PI, Owens IS, Burchell B, Bock KW, Bairoch A, Belanger A, Fournel-Gigleux S, Green M, Hum DW, Iyanagi T: The UDP glycosyltransferase gene superfamily: recommended nomenclature update based on evolutionary divergence. Pharmacogenetics. 1997, 7: 255-269. 10.1097/00008571-199708000-00001.

Meech R, Mackenzie PI: Structure and function of uridine diphosphate glucuronosyltransferases. Clin Exp Pharmacol Physiol. 1997, 24: 907-915. 10.1111/j.1440-1681.1997.tb02718.x.

Lazard D, Zupko K, Poria Y, Nef P, Lazarovits J, Horn S, Khen M, Lancet D: Odorant signal termination by olfactory UDP-glucuronosyltransferase. Nature. 1991, 349: 790-793. 10.1038/349790a0.

Ahmad S, Forgash AJ: Nonoxidative enzymes in the metabolism of insecticides. Drug Metab Rev. 1976, 5: 141-164. 10.3109/03602537608995840.

Ahmad SA, Hopkins TL: β-glucosylation of plant phenolics by phenol β-glucosyltransferase in larval tissues of the tobacco hornworm, Manduca sexta (L.). Insect Biochem Mol Biol. 1993, 23: 581-589. 10.1016/0965-1748(93)90031-M.

Rausell C, Llorca J, Real MD: Separation by FPLC chromatofocusing of UDP-glucosyltransferases from three developmental stages of Drosophila melanogaster. Arch Insect Biochem Physiol. 1997, 34: 347-358. 10.1002/(SICI)1520-6327(1997)34:3<347::AID-ARCH8>3.0.CO;2-R.

Real M, Ferre J, Chapa F: UDP-glucosyltransferase activity toward exogenous substrates in Drosophila melanogaster. Anal Biochem. 1991, 194: 349-352. 10.1016/0003-2697(91)90239-P.

Bull DL, Whitten CJ: Factors influencing organophosphorus insecticide resistance in tobacco budworms. J Agric Food Chem. 1972, 20: 561-564. 10.1021/jf60181a061.

Kramer K, Hopkins T: Tyrosine metabolsim for insect cuticle tanning. Arch Insect Biochem Physiol. 1987, 6: 279-301. 10.1002/arch.940060406.

Hopkins TL, Kramer KJ: Insect cuticle sclerotization. Annu Rev Entomol. 1992, 37: 273-302. 10.1146/annurev.en.37.010192.001421.

Wang Q, Hasan G, Pikielny CW: Preferential expression of biotransformation enzymes in the olfactory organs of Drosophila melanogaster, the antennae. J Biol Chem. 1999, 274: 10309-10315. 10.1074/jbc.274.15.10309.

Teresa L, David R, Reilly O: Functional and phylogenetic analyses of a putative Drosophila melanogaster UDP-glycosyltransferase gene. Insect Biochemistry and Molecular Biology. 2002, 32: 1597-1604. 10.1016/S0965-1748(02)00080-2.

Li Y, Baldauf S, Lim EK, Bowles DJ: Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana. J Biol Chem. 2001, 276: 4338-4343. 10.1074/jbc.M007447200.

Mackenzie P: Expression of chimeric cDNAs in cell cultured a region of UDP glucuronosyltransferase involved in substrate selection. J Biol Chem. 1990, 265: 3432-3435.

Radominska-Pandya A, Czernik PJ, Little JM, Battaglia E, Mackenzie PI: Structural and functional studies of UDP-glucuronosyltransferases. Drug Metab Rev. 1999, 31: 817-899. 10.1081/DMR-100101944.

Jackson MR, Nilsson T, Peterson PA: Identification of a consensus motif for retention of transmembrane proteins in the endoplasmic reticulum. EMBO J. 1990, 9 (10): 3153-3162.

Mackenzie PI, Owens IS: Differential induction and glycosylation of UDP-glucuronosyltransferase. Biochem Soc Trans. 1984, 12 (1): 54-55.

Nilsson T, Jackson M, Peterson PA: Short cytoplasmic sequences serve as retention signals for transmembrane proteins in the endoplasmic reticulum. Cell. 1989, 58: 707-718. 10.1016/0092-8674(89)90105-0.

Shin J, Dunbrack RL, Lee S, Strominger JL: Signals for retention of transmembrane proteins in the endoplasmic reticulum studied with CD4 truncation mutants. Proc Natl Acad Sci USA. 1991, 88: 1918-1922. 10.1073/pnas.88.5.1918.

Yokota H, Yuasa A, Sato R: Topological disposition of UDP-glucuronyltransferase in rat liver microsomes. J Biochem. 1992, 112: 192-196.

Luque T, Okano K, O'Reilly DR: Characterization of a novel silkworn (Bombyx mori) phenol UDP-glucosyltransferase. Eur J Biochem. 2002, 269: 819-825. 10.1046/j.0014-2956.2001.02723.x.

Fujimoto N: On the physiological functions of the genes concerning the formation and the translocation of the pigments of green cocoon in silkworm larvae. J Seric Sci Jpn. 1972, 32: 338-342.

Yasumori T, Nakajimab K, Nagayasua K, Takabayashib C: Flavonoid 5-glucosides from the cocoon shell of the silkworm, Bombyx mori. Phytochemistry. 2002, 59: 275-278. 10.1016/S0031-9422(01)00477-0.

Hopkins TL, Ahmad SA: Flavonoid wing pigments in grasshoppers. Experientia. 1991, 47: 1089-1091. 10.1007/BF01923349.

Lahtinen M, Kapari L, Kentta J: Newly hatched neonate larvae can glycosylate: the fate of Betula pubescens bud flavonoids in first instar Epirrita autumnata. J Chem Ecol. 2006, 32: 537-546. 10.1007/s10886-005-9015-6.

Salminen JP, Lahtinen M, Lempa K, Kapari L, Haukioja E, Pihlaja K: Metabolic modifications of birch leaf phenolics by an herbivorous insect: detoxification of flavonoid aglycones via glycosylation. Z Naturforsch [C]. 2004, 59 (5-6): 437-444.

Wiesen B, Krug E, Fiedler K, Wray V, Proksch P: Sequestration of host-plant-derived flavonoids by lycaenid butterfly Polyommatus icarus. J Chem Ecol. 1994, 20: 2523-2538. 10.1007/BF02036189.

Chikara H, Hiroshi O, Yasumori T, Kotaro K, Masatoshi N: Regioselective formation of quercetin 5-O-glucoside from orally administered quercetin in the silkworm, Bombyx mori. Phytochemistry. 2008, 69: 1141-1149. 10.1016/j.phytochem.2007.11.009.

Thompson JD, Higgins DG, Gibson TJ: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994, 22: 4673-4680. 10.1093/nar/22.22.4673.

Saitou N, Nei M: The neighbour-joining method: a new method for constructing phylogenetic trees. Mol Biol Evol. 1987, 4: 406-425.

Tamura K, Dudley J, Nei M: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007, 24 (8): 1596-1599. 10.1093/molbev/msm092.

Xia Q, Cheng D, Duan J, Wang G, Cheng T, Zha X, Liu C, Zhao P, Dai F, Zhang Z, He N, Zhang L, Xiang Z: Microarray-based gene expression profiles in multiple tissues of the domesticated silkworm, Bombyx mori. Genome Biol. 2007, 8: R162-10.1186/gb-2007-8-8-r162.

Thông tin
Thông tin xuất bản

Springer Science and Business Media LLC

Thông tin tác giả