Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery

Journal of Cell Biology - Tập 180 Số 5 - Trang 897-904 - 2008
Enko N. Kiprilov1, Aashir Awan1,2,3, Romain Desprat4, Michelle Velho5, Christian Clément2, Anne Grete Byskov3, Claus Yding Andersen3, Peter Satir1, Eric E. Bouhassira4,6, Søren T. Christensen2, Rhoda Elison Hirsch1,5
11Department of Anatomy and Structural Biology
24Institute of Molecular Biology, University of Copenhagen, DK-2100 Copenhagen OE, Denmark
35Laboratory of Reproductive Biology, Rigshospitalet, DK-2100 Copenhagen OE, Denmark
43Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461
52Department of Medicine,
6Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461

Tóm tắt

Human embryonic stem cells (hESCs) are potential therapeutic tools and models of human development. With a growing interest in primary cilia in signal transduction pathways that are crucial for embryological development and tissue differentiation and interest in mechanisms regulating human hESC differentiation, demonstrating the existence of primary cilia and the localization of signaling components in undifferentiated hESCs establishes a mechanistic basis for the regulation of hESC differentiation. Using electron microscopy (EM), immunofluorescence, and confocal microscopies, we show that primary cilia are present in three undifferentiated hESC lines. EM reveals the characteristic 9 + 0 axoneme. The number and length of cilia increase after serum starvation. Important components of the hedgehog (Hh) pathway, including smoothened, patched 1 (Ptc1), and Gli1 and 2, are present in the cilia. Stimulation of the pathway results in the concerted movement of Ptc1 out of, and smoothened into, the primary cilium as well as up-regulation of GLI1 and PTC1. These findings show that hESCs contain primary cilia associated with working Hh machinery.

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Journal of Cell Biology

Tập 180 Số 5

897-904

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