Inhibition of 5α-Reductase, IL-6 Secretion, and Oxidation Process of Equisetum debile Roxb. ex Vaucher Extract as Functional Food and Nutraceuticals Ingredients

Nutrients - Tập 9 Số 10 - Trang 1105
Wantida Chaiyana1, Chanun Punyoyai1, Suvimol Somwongin1, Pimporn Leelapornpisid1, Kornkanok Ingkaninan2, Neti Waranuch2, Jukkarin Srivilai2, Natthawut Thitipramote3, Wudtichai Wisuitiprot4, Roswitha Schuster5, Karl‐Heinz Wagner5, Monika Mueller5
1Department of Pharmaceutical Science, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand
2Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000, Thailand
3School of Cosmetic Science, Mae Fah Luang University, Muang, Chiang Rai 57100, Thailand
4Department of Thai Traditional Medicine, Sirindhorn College of Public Health Phitsanulok 65130, Thailand
5Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, Vienna 1090, Austria

Tóm tắt

This study aims to investigate the biological activities related to hair loss of Equisetum debile extracts, including 5α-reductase inhibition, interleukin-6 (IL-6) secretion reduction, and anti-oxidation. E. debile extracts were obtained by maceration in various solvents. Crude extract (CE) was obtained by maceration in 95% ethanol. Chlorophyll-free extract (CF) was the CE which of the chlorophyll has been removed by electrocoagulation. Hexane extract (HE), ethyl acetate extract (EA), and ethanolic extract (ET) were fraction extracts obtained from maceration in hexane, ethyl acetate, and 95% ethanol, respectively. The extracts were investigated for inhibitory activity against 5α-reductase and IL-6 secretion. Total phenolic contents (TPC) were investigated and antioxidant activities were determined by means of 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2′-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) assays. The inhibition of lipid peroxidation was determined by the ferric thiocyanate method. The cytotoxicity of the extracts on dermal papilla cells and irritation test by hen's egg test chorioallantoic membrane assay were also investigated. All extracts could inhibit 5α-reductase and decrease IL-6 secretion in lipopolysaccharide-stimulated macrophage. The antioxidant activity of E. debile extracts was directly related to their TPC. ET which contained the highest TPC (68.8 ± 6.7 mg GA/g) showed the highest equivalent concentration (EC1) of 289.1 ± 26.4 mM FeSO4/g, TEAC of 156.6 ± 34.6 mM Trolox/g, and 20.0 ± 6.0% DPPH inhibition. However, EA exhibited the highest inhibition against lipid peroxidation (57.2 ± 0.4%). In addition, EA showed no cytotoxicity on dermal papilla cell line and no irritation on chorioallantoic membrane of hen’s eggs. In conclusion, EA was suggested as the most attractive ingredients for functional food and nutraceuticals because of the high inhibitory activity against 5α-reductase, IL-6 secretion, and lipid peroxidation inhibition.

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Tài liệu tham khảo

Stough, 2005, Psychological effect, pathophysiology, and management of androgenetic alopecia in men, Mayo Clin. Proc., 80, 1316, 10.4065/80.10.1316

Dawber, 1987, Aetiology and pathophysiology of hair loss, Dermatology, 175, 23, 10.1159/000248896

Cotsarelis, 2001, Towards a molecular understanding of hair loss and its treatment, Trends Mol. Med., 7, 293, 10.1016/S1471-4914(01)02027-5

Matsuda, 2001, Anti-androgenic Activity of Myricae Cortex—Isolation of Active Constituents from Bark of Myrica rubra, Biol. Pharm. Bull., 24, 259, 10.1248/bpb.24.259

Ahn, 2012, Effect of IGF-I on hair growth is related to the anti-apoptotic effect of IGF-I and up-regulation of PDGF-A and PDGF-B, Ann. Dermatol., 24, 26, 10.5021/ad.2012.24.1.26

Whiting, 2001, Possible mechanisms of miniaturization during androgenetic alopecia or pattern hair loss, J. Am. Acad. Dermatol., 45, S81, 10.1067/mjd.2001.117428

Otberg, 2007, Androgenetic alopecia, Endocrinol. Metab. Clin. N. Am., 36, 379, 10.1016/j.ecl.2007.03.004

Kwack, 2012, Dihydrotestosterone-inducible IL-6 inhibits elongation of human hair shafts by suppressing matrix cell proliferation and promotes regression of hair follicles in mice, J. Investig. Dermatol., 132, 43, 10.1038/jid.2011.274

Wood, 2009, Senile hair graying: H2O2-mediated oxidative stress affects human hair color by blunting methionine sulfoxide repair, FASEB J., 23, 2065, 10.1096/fj.08-125435

Naito, 2008, Lipid peroxides induce early onset of catagen phase in murine hair cycles, Int. J. Mol. Med., 22, 725

Mehra, 1959, A note on chromosome numbers in some Indian species of Equisetum, Am. Fern J., 49, 86, 10.2307/1545927

Asgarpanah, 2012, Phytochemistry and pharmacological properties of Equisetum arvense L., J. Med. Plants Res., 6, 3689

Kamal, 2009, Ethnobotanically important plants of district Bannu, Pakistan, Pak. J. Biol. Sci., 15, 87

Bandaranayake, 2004, Hair loss remedies-separating fact from fiction, Cutis, 73, 107

Enshaieh, 2008, Comparison between the efficacy of a herbal drug and minoxidil in the treatment of the telogen effluvium, J. Cell Tissue Res., 8, 1253

Schaffner, 1930, Geographic distribution of the species of Equisetum in relation to their phylogeny, Am. Fern J., 20, 89, 10.2307/1543867

Jamal, 2012, Medicinal plants used in traditional folk recipes by the local communities of Kaghan valley, Mansehra, Pakistan, Indian J. Tradit. Knowl., 11, 634

Sarkar, 2012, Cytotoxic, antibacterial and free radical scavenging activity studies of the solvent extracts of aerial stems of Equisetum debile Roxb., Int. J. Chem. Sci., 10, 19

Srivilai, 2016, A new label-free screen for steroid 5α-reductase inhibitors using LC-MS, Steroids, 116, 67, 10.1016/j.steroids.2016.10.007

Poletti, 1998, Presence of 5α-Reductase isozymes and aromatase in human prostate cancer cells and in benign prostate hyperplastic tissue, Prostate, 34, 283, 10.1002/(SICI)1097-0045(19980301)34:4<283::AID-PROS6>3.0.CO;2-I

Mueller, 2010, Anti-inflammatory activity of extracts from fruits, herbs and spices, Food Chem., 122, 987, 10.1016/j.foodchem.2010.03.041

Li, 2009, 5 alpha-reductase and aromatase inhibitory constituents from Brassica rapa L. pollen, Chem. Pharm. Bull., 57, 401, 10.1248/cpb.57.401

Tachakittirungrod, 2007, Study on antioxidant activity of certain plants in Thailand: Mechanism of antioxidant action of guava leaf extract, Food Chem., 103, 381, 10.1016/j.foodchem.2006.07.034

Brem, 2004, Antioxidant dehydrotocopherols as a new chemical character of Stemona species, Phytochemistry, 65, 2719, 10.1016/j.phytochem.2004.08.023

Saeio, 2011, Antityrosinase and antioxidant activities of essential oils of edible Thai plants, Drug Discov. Ther., 5, 144, 10.5582/ddt.2011.v5.3.144

Osawa, 1981, A novel type of antioxidant isolated from leaf wax of Eucalyptus leaves, Agric. Biol. Chem., 45, 735

Van, 2011, Cell sensitivity assays: The MTT assay, Cancer Cell Cult. Methods Protoc., 731, 237, 10.1007/978-1-61779-080-5_20

Luepke, 1986, The HET-CAM test: An alternative to the Draize eye test, Food Chem. Toxicol., 24, 495, 10.1016/0278-6915(86)90099-2

Steiling, 1999, The HET–CAM, a useful in vitro assay for assessing the eye irritation properties of cosmetic formulations and ingredients, Toxicol. In Vitro, 13, 375, 10.1016/S0887-2333(98)00091-5

Freire, 2015, Action of silver nanoparticles towards biological systems: Cytotoxicity evaluation using hen’s egg test and inhibition of Streptococcus mutans biofilm formation, Int. J. Antimicrob. Agents, 45, 183, 10.1016/j.ijantimicag.2014.09.007

2008, Chemical Composition of the Essential Oils of Equisetum palustre L. and Equisetum telmateia Ehrh, J. Essent. Oil Res., 20, 310, 10.1080/10412905.2008.9700020

Sarkar, 2014, Isolation of Two Steroids and Two Flavonoids Having Antioxidant, Antibacterial and Cytotoxic Properties from Aerial Stems of Equisetum Debile Roxb., Nat. Univ. J. Sci., 1, 31

Li, 2007, Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae, Food Chem., 102, 771, 10.1016/j.foodchem.2006.06.022

Awad, 1998, Phytosterol feeding induces alteration in testosterone metabolism in rat tissues, J. Nutr. Biochem., 9, 712, 10.1016/S0955-2863(98)00076-X

Kranner, 2002, Revival of a resurrection plant correlates with its antioxidant status, Plant J., 31, 13, 10.1046/j.1365-313X.2002.01329.x

Prior, 2005, Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements, J. Agric. Food Chem., 53, 4290, 10.1021/jf0502698

Villano, 2007, Radical scavenging ability of polyphenolic compounds towards DPPH free radical, Talanta, 71, 230, 10.1016/j.talanta.2006.03.050

Vitrac, 2009, Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays, J. Agric. Food Chem., 57, 1768, 10.1021/jf803011r

Abdelkader, 2015, Critical appraisal of alternative irritation models: Three decades of testing ophthalmic pharmaceuticals, Br. Med. Bull., 113, 59, 10.1093/bmb/ldv002

Liu, 2009, Anti-Androgenic Activity of Fatty Acids, Chem. Biodivers., 6, 503, 10.1002/cbdv.200800125

Arunmathi, 2017, Analysis of bioactive compounds in methanol extract of Aplotaxis auriculata rhizome using GC-MS, J. Pharmacogn. Phytochem., 6, 243

Sabeva, 2011, Phytosterols differentially influence ABC transporter expression, cholesterol efflux and inflammatory cytokine secretion in macrophage foam cells, J. Nutr. Biochem., 22, 777, 10.1016/j.jnutbio.2010.07.002

Hunter, 2015, IL-6 as a keystone cytokine in health and disease, Nat. Immunol., 16, 448, 10.1038/ni.3153

Correia, 2005, Characterisation of polyphenols by HPLC-PAD-ESI/MS and antioxidant activity in Equisetum telmateia, Phytochem. Anal., 16, 380, 10.1002/pca.864

Boots, 2008, Health effects of quercetin: From antioxidant to nutraceutical, Eur. J. Pharmacol., 585, 325, 10.1016/j.ejphar.2008.03.008

Kanchanapoom, 2007, Megastigmane glucosides from Equisetum debile and E. diffusum, Chem. Pharm. Bull., 55, 1277, 10.1248/cpb.55.1277