Expressions of Aquaporin‐2, Vasopressin Type 2 Receptor, Transient Receptor Potential Channel Vanilloid (TRPV)1, and TRPV4 in the Human Endolymphatic Sac

Laryngoscope - Tập 117 Số 4 - Trang 695-698 - 2007
Daizo Taguchi1, Taizo Takeda1, Akinobu Kakigi1, Masaya Takumida2, Rie Nishioka1, Hiroya Kitano3
1Department of Otolaryngology, Kochi Medical School, Nankoku, Kochi, Japan
2Department of Otolaryngology, Hiroshima University, Faculty of Medicine, Hiroshima, Hiroshima, Japan
3Division of Otolaryngology, Head and Neck Surgery, Tottori University, Faculty of Medicine, Yonago, Tottori, Japan

Tóm tắt

AbstractObjective: To localize aquaporin (AQP)2, vasopressin type 2 receptor (V2‐R), and transient receptor potential channel vanilloid subfamily 1, 4 (TRPV1, TRPV4) in the human endolymphatic sac (ES).Methods: Three samples of human ES were sampled during the removal of vestibular schwannoma by way of the translabyrinthine approach. The samples were immediately fixed in 4% paraformaldehyde and embedded in OCT compound; immunohistochemistry was performed with AQP2, V2‐R, TRPV1, and TRPV4 polyclonal antibodies.Results: AQP2, V2‐R, TRPV1, and TRPV4 proteins were detected in the epithelial layer of the ES but were not observed in connective tissue around the ES. TRPV1 was also expressed in blood vascular endothelial cells of the connective tissue of ES.Conclusions: AQP2, V2‐R, and TRPV4 were expressed in the luminal epithelium of human ES. The same characteristic distribution of water and ion channels is seen in the kidney, where a significant amount of fluid is filtrated and resorbed. ES probably plays an active role in the homeostasis of the endolymph.

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Tài liệu tham khảo

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Laryngoscope

Tập 117 Số 4

695-698

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