Cyclooxygenase-2 Expression in Inflammatory Lung Lesions of Nonhuman Primates

Veterinary Pathology - Tập 37 Số 5 - Trang 512-516 - 2000
Kanwar Nasir M. Khan, Kristina M. Stanfield, Divna Trajković, R. K. Harris

Tóm tắt

Mammalian cells contain two related but unique isoforms of cyclooxygenase (COX-1 and COX-2). COX-1 is expressed constitutively in a majority of tissues and is involved in the production of prostaglandins (PGs) that modulate normal physiologic functions. COX-2 is inducible by various stimuli and is involved in the production of PGs that modulate physiologic events in development, cell growth, and inflammation. With the exception of peribronchial glands and chondrocytes of peribronchial cartilage, COX-2 is not detectable in the normal lung of nonhuman primates. We evaluated COX-2 expression by immunohistochemical methods in the inflammatory lesions of two cynomolgus monkeys ( Macaca fascicularis) with acute severe pneumonia. Both monkeys exhibited acute severe bronchopneumonia; histologically, lung lesions were characterized by infiltration of large numbers of neutrophils and fewer macrophages, mild bronchial epithelial hyperplasia, and slight type-2 pneumocyte hyperplasia. In both monkeys, mild to marked COX-2 immunoreactivity was detected within the cytoplasm of macrophages, bronchial epithelial cells, type-2 pneumocytes, and endothelial cells of blood vessels. No COX-2 immunoreactivity was detectable in the neutrophils that constituted >90% of the inflammatory cells. These observations suggest that in acute inflammatory lung lesions in nonhuman primates 1) COX-2 is induced in the bronchial and alveolar epithelial cells, 2) macrophages are the primary inflammatory cells that exhibit COX-2, and 3) neutrophils do not express COX-2.

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Tài liệu tham khảo

Adawi A, 1998, Am J Pathol, 152, 651

10.1002/1097-0142(19940901)74:5<1546::AID-CNCR2820740509>3.0.CO;2-F

10.1038/sj.bjp.0700963

Brannon TS, 1997, Am J Physiol, 274, L66

10.1096/fasebj.12.12.1063

10.1165/ajrcmb.18.4.2939

10.1001/archinte.1996.00440010035006

10.1016/S0021-9258(20)30087-9

10.1177/019262339802600504

Maloney CG, 1998, J Immunol, 160, 1402, 10.4049/jimmunol.160.3.1402

10.1073/pnas.91.8.3228

10.1182/blood.V89.5.1621

10.1073/pnas.91.25.12013

10.1038/sj.bjp.0701283

10.1172/JCI117866

Viagno T, 1988, Eicosanoids, 1, 73

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Thông tin xuất bản

Veterinary Pathology

Tập 37 Số 5

512-516

Thông tin tác giả