Chemical Composition and Recrystallization of Epicuticular Waxes: Coiled Rodlets and Tubules

Plant Biology - Tập 2 Số 4 - Trang 462-470 - 2000
Iris Meusel1, Christoph Neinhuis1, Claus Markstädter2, Wilhelm Barthlott1
1Botanisches Institut und Botanischer Garten, Bonn, Germany
2Julius‐von‐Sachs‐Institut für Biowissenschaften, Lehrstuhl für Botanik II, Würzburg, Germany

Tóm tắt

Abstract: Coiled rodlets characterize several non‐related taxa within the angiosperms. They often occur together with tubules but sometimes also with platelets or transitional forms between them. The ultrastructure chemistry, and recrystallization of epicuticular waxes of three species were investigated by high‐resolution scanning electron microscopy, gas chromatography, and mass spectrometry. Whereas Buxus sempervirens (Buxaceae) and Chrysanthemum segetum (Asteraceae) show coiled rodlets in combination with tubules, Leymus arenarius (Poaceae) exhibits tubules but no coiled rodlets. Chemical analyses reveal that the predominating β‐diketones of all species differ completely in their molecular structure. Those of the former two species are mainly substituted in carbon atom positions up to 12. In contrast, the wax of L. arenarius contains only hentriacontane‐14,16‐dione and 25‐hydroxy‐hentriacontane‐14,16‐dione. Standard solutions of the total waxes from B. sempervirens, C. segetum and L. arenarius, the purified β‐diketone fraction from C. segetum and hentriacontane‐14,16‐dione from Secale cereale were taken for recrystallization experiments under different conditions in relation to solvent and crystallization velocity. It was demonstrated that coiled rodlets grew exclusively from total waxes of B. sempervirens and C. segetum, and its β‐diketone fraction but never from L. arenarius wax or pure hentriacontane‐14,16‐dione. The recrystallization experiments pointed out that conditions, such as the chemical environment and physical factors, strongly influence the formation of coiled rodlets and tubules. It is concluded that coiled rodlets are formed by self‐assembly in close dependence on the position of β‐diketo substitution. The future role of β‐diketones in the classification of coiled rodlets within wax crystals is discussed.

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Tài liệu tham khảo

Baker E. A., 1982, The Plant Cuticle, 139

Barthlott W., 1990, Scanning electron microscopy in taxonomy and functional morphology, 69

Jeffree C. E., 1986, Insects and the Plant Surface, 23

Barthlott W., 1998, Classification and terminology of plant epicuticular waxes., Bot. J. Linn. Soc., 126, 137, 10.1111/j.1095-8339.1998.tb02529.x

10.1111/j.1469-8137.1975.tb01417.x

10.1007/BF00199686

10.1111/j.1438-8677.1995.tb00840.x

10.1139/cjb-77-5-706

10.1088/0022-3727/21/9/016

10.1088/0022-3727/21/9/018

Kreger D. R., 1948, An X‐ray study of waxy coating from plants., Recueil des Traveaux botaniques Neerlandais, 42, 606

Bianchi G., 1995, Waxes: chemistry, molecular biology and functions, 177

Walton T. J., 1990, Lipids, membranes and aspects of photobiology, 105

Riederer M., 1996, Plant Cuticles an Integrated Functional Approach, 189

10.1016/S0367-2530(17)30709-0

10.1016/S0031-9422(00)97477-6

10.1139/b96-052

10.1016/0031-9422(96)00180-X

Riederer M., 1989, Ecological Studies, 157

10.1139/b81-091

10.1016/0031-9422(75)80362-1

Evans D., 1980, Contributions toward a classification of Rhododendron, 187

10.1007/BF00387646

10.1016/0031-9422(73)85124-6

Tulloch A. P., 1976, Chemistry and biochemistry of natural waxes, 236

10.1139/b80-302

10.1007/BF02906259

10.1016/B978-0-12-215050-0.50008-4

Jetter R., 1993, Chemische Zusammensetzung, Struktur und Bildung röhrchenförmiger Wachskristalle auf Pflanzenobeflächen

10.1016/0031-9422(73)80597-7

Ditsch F., 1997, Mikromorphologie der Epicuticularwachse und das System der Dilleniidae und Rosidae., Trop. Subtrop. Pflanzenwelt, 97, 1

10.1111/j.1095-8339.1960.tb02509.x

10.1016/S0022-5320(74)90069-0

10.1016/0031-9422(76)85119-9

10.1071/CH9640464

10.1007/BF02906294

10.1179/jbr.1995.18.3.399

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Plant Biology

Tập 2 Số 4

462-470

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