Development of a new method for the preparation of an acellular allodermis, quality control and cytotoxicity testing

Cell and Tissue Banking - Tập 18 - Trang 153-166 - 2017
J. Dragúňová1, P. Kabát2,3, P. Babál4, H. Mrázová4, M. Boháč5, Ľ. Krajčíová6, J. Koller1
1Department of Burns and Reconstructive Surgery, Faculty of Medicine, Comenius University Bratislava, Bratislava, Slovak Republic
2Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic
3Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovak Republic
4Institute of Pathological Anatomy, Faculty of Medicine, Comenius University, Bratislava, Slovak Republic
5Department of Plastic, Reconstructive and Aesthetic Surgery, Faculty of Medicine, Comenius University, Bratislava, Slovak Republic
6Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University, Bratislava, Slovak Republic

Tóm tắt

Demand for use of acellular allodermis is high but commercially appropriate products are not used routinely because of very high price and limited availability. These facts did motivate us to prepare acellular allodermis using a new, simple and less expensive method. We have developed a original method for preparation of acellular allogeneic dermis based on action of a proteolytic enzyme in combination with distilled water. Hypotonic environment in comparison with SDS or Triton ansure no toxicity of the final product. Trials for determination of optimal trypsin concentrations, temperature and time of action were performed. According to our results, the use of 2.5% trypsin/EDTA solution overnight at +4 °C was proving to be optimal. The histology confirmed absence of cells in the prepared dermis. No toxicity of final acellular dermis was confirmed by three independent tests (agar diffusion test contact cytotoxicity test and grow curve). The prepared acellular dermis seems to be suitable not only for direct clinical use, but it can be used as a scaffold for cell cultivation as well.

Tài liệu tham khảo

Alexander JW, Sykes NS, Mitchell MM, Fisher MW (1973) Concentration of selected intravenously administered antibiotics in experimental surgical wounds. J Trauma 13(5):423–434

Badylak SF, Taylor D, Uygun K (2011) Whole organ tissue engineering: decellularization and recellularization of three-dimensional matrix scafflods. Annu Rev Biomed Eng 13:27–53. doi:10.1146/annurev-bioeng-071910-124743

Bhavsar D, Tenenhaus M (2008) The use of acellular dermal matrix for coverage of exposed joint and extensor mechanism in thermally injured patients with few opinion. Open Acces J Plast Surg 24(8):333–342

Buinewicz B, Rosen B (2004) Acellular cadaveric dermis (AlloDerm): a new alternative for abdominal hernia repair. Ann Plast Surg 52(2):188–194. doi:10.1097/01.sap0000100895.41198.27

Calota DR, Nitescu C, Florescu IP, Lascar I (2012) Surgical management of extensive burns treatment using allografts. J Med Life 5(4):486–490

Castagnolli C, Stella M, Magliacani G, Alasia ST, Richiardi P (1990) Anomalous expression of HLA calss II molecules on keratinocytes and fibroblasts in hypertrophic scars consequent to thermal injury. Clin Exp Immunol 82(2):350–354

Cebotari S, Tudorache I, Jaekel T, Hilfiker A, Dorfman S, Ternes W, Haverich A, Lichtenberg A (2010) Detergent decellularization of hearth valves for tissue engineering: toxicological effect of residual detergents on human endothelial cells. Aritif Organs 34(3):206–210. doi:10.1111/j.1525-1594.2009.00796.x

Chaplin JM, Constantino PD, Wolpoe ME, Baderson JB, Es Griffey, Zhang WX (1999) Use of an acellular dermal allograft for dural replacement. An experimental study. Neurosurgery 45(2):320–327

Crapo PM, Gilbert TW, Badylak SF (2011) An overview of tissue and whole organ decellularization processes. Biomaterials 32(12):3233–3243. doi:10.1016/j.biomaterials.2011.01.057

Eberli D, Rodriguez S, Atala A, Yoo JJ (2010) In vivo evaluation of acellular human dermis for abdominal wall reapir. J Biomed Mater Res A 93(4):1527–1538. doi:10.1002/jbm.a.32636

Eldad A, Burt A, Clarke JA, Gusterson B (1987) Cultured epithelium as a skin substitute. Burns Incl Therm Inj 13(3):173–180

El-Hadidy MR, El- Hadidy AR (2003) Clinical and histological evaluation of acellular allograft dermal matrix in full-thickness burns Egypt. J Plast Reconstr Surg 27(1):61–72

Fu RH, Wang YC, Liu SP, Shih TR, Lin HL, Chen YM, Sung JH, Lu CH, Wei JR, Wang ZW, Huang SJ, Tsai CH, Shyu WC, Lin SZ (2014) Decellularization and Recellularization Technologies in tissueengineering. Cell Transpl 23(4–5):621–630. doi:10.3727/096368914X678382

Greenleaf G, Cooper ML, Hansbrough JF (1991) Microbial contamination in allografted wound beds in patients with burns. J Burn Care Rehabil 12(5):442–446

Hogg P, Rooney P, Ingham E, Kearney JN (2013) Development of a decellularised dermis. Cell Tissue Bank 14(3):465–474. doi:10.1007/s10561-012-9333-1

Hogg P, Rooney P, Leow- Dyke S, Brown C, Ingham E, Kearney JN (2015) Development of a terminally sterilised decellularised dermis. Cell Tissue Bank 16(3):351–359. doi:10.1007/s10561-014-9479-0

Hrebikova H, Diaz D, Mokry J (2015) Chemical decellularization: a promising approach for preparation of extracellular matrix. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 159(1):12–17

Jones I, Currie L, Martin R (2002) A guide to biological skin substitutes. Br J Plast Surg 55(3):185–193. doi:10.1054/bjps.2002.3800

Keane TJ, Swinehart IT, Badylak SF (2015) Methods of tissue decellularization used for preparation of biologic scaffolds and in vivo relevance. Methods 84:25–34. doi:10.1016/j.ymeth.2015.03.005

Macadam SA, Lennox PA (2012) Acellular dermal matrices: use in reconstructive and aesthetic breast surgery. Can J Plast Surg 20(2):75–89

Macchiarini P, Jungelbluth P, Go T, Asnaghi MA, Rees LE, Cogan TA, Dodson A, Martorell J, Bellini S, Parnigotto PP, Dickinson SC, Hollander AP, Mantero S, Conconi MT, Birchal MA (2008) Clinical transplantation of a tissue- engineered airway. Lancet 372(9655):2023–2030. doi:10.1016/S0140-6736(08)61598-6

Medalie DA, Tompkins RG, Morgan JR (1996) Evaluation of acellular human dermis as a dermal analog in a composite skin graft. ASAIO J 42(5):M455–M462

Mizuno H, Takeda A, Uchinuma E (1999) Creation of an acellular dermal matrix from frozen skin. Aesthetic Plast Surg 23(5):316–322

Moore MA, Samsell B, Wallis G, Triplett S, Chen S, Jones AL, Qin X (2015) Decellularization of human dermis using non-denaturing aninic detergent and endonuclease: a review. Cell Tissue Bank 16(2):249–259. doi:10.1007/s10561-014-9467-4

Mrázová H, Koller J, Fujeríková G, Babál P (2014) Structural changes of skin and amnion grafts for transplantation purposes following different doses of irradiation. Cell Tissue Bank 15(3):429–433. doi:10.1007/s10561-013-9407-8

Prasertsung I, Kanokpanont S, Bunaprasert T, Thanakit V, Damrongsakkul S (2008) Development of acellular dermis from porcine skin using periodic pressurized technique. J Biomed Mater Res B Appl Biomater 85((1):210–219. doi:10.1002/jbm.b.30938

Rada B, Závada J (1962) Screening-test for cytostatic and virostatic substances. Neoplasma 9:57–65

Rowan MP, Cancio LC, Elster EA, Burmeister DM, Rose LF, Natesan S, Chan RK, Christy RJ, Chung KK (2015) Burn wound healing and treatment: review and advancements. Crit Care 19:243–263. doi:10.1186/s13054-015-0961-2

Sedmak DD, Orosz CG (1991) The role of vascular endothelial cells in transplantation. Arch Pathol Lab Med 115(3):260–265

Sheridan RL, Tompkins RG (1999) Skin substitutes in Burns. Burns 25(2):97–103. doi:10.1016/S0305-4179(98)00176-4

Shukla A, Dey N, Nandi P, Ranjan M (2015) Acellular dermis as a dermal matrix of tissue engineered in substitute for burns treatment. Ann Public Health Res 2(3):1023–1040

Sterling JP, Heimbach DM, Gibran NS (2010) Management of the burn wound. In: Brit LD, Peitzman AB, Barie PS, Jurkovich GJ Acute care surgery: priciples and practise, vol 15. Decker, pp 1–13. doi: 10.2310/7800.S07C15

Takami Y, Matsuda T, Yoshitake M, Hanumadass M, Walter RJ (1996) Dispase/detergent-treated dermal matrix as a dermal substitute. Burns 22(3):182–190

Tall H (1999) Subgingival acellular dermal matrix allograft for the treatment of gingival recession: a case report. J Periodontol 70(9):1118–1124. doi:10.1902/jop.1999.70.9.1118

Terino EO (2001) Alloderm acellular dermal graft: application in aesthetic soft-tissue augmentation. Clin Plast Surg 28(1):83–99

Vitteková M, Dragúňová J, Kabát P, Božiková M, Bakoš J, Koller J (2014) Cytotoxicity testing of scaffolds potentially suitable for the preparation of three-dimensional skin substitutes. Cell Tissue Bank 15(3):345–355. doi:10.1007/s10561-013-9390-0

Wainwright DJ (1995) The use of an allograft dermal matrix (AlloDerm) in the management of full thickness burns. Burns 21(4):243–248. doi:10.1016/0305-417(95)93866-I

Walter RJ, Matsuda T, Reyes HM, Walter JM, Hanumadass M (1998) Characterization of acellular dermal matrices (ADMs) prepared by two different methods. Burns 24(2):104–113. doi:10.1016/S0305-4179(97)00110-1

Wong ML, Leach JK, Athanasiou KA, Griffiths LG (2011) The role of protein solubilization in antigen removal from xenogenic tissue for heart valve tissue engineering. Biomaterials 32(32):8129–8138. doi:10.1016/j.biomaterials.2011.07.030

Zellner PR (1974) Konservierte Leichenhaut zur Deckung grossflachiger drittgradiger Verbrennungswunden/P.R. Zellner//Zentralbl. Chir. 74. -Bd.99, H.35: 1105-1107

Thông tin
Thông tin xuất bản

Cell and Tissue Banking

Tập 18

153-166

Thông tin tác giả