A New Group-Training Procedure for Habituation Demonstrates That Presynaptic Glutamate Release Contributes to Long-Term Memory in <i>Caenorhabditis elegans</i>

Learning and Memory - Tập 9 Số 3 - Trang 130-137 - 2002
Jacqueline K. Rose1, Karla R. Kaun2, Catharine H. Rankin3
1#N#Department of Psychology and Brain Research Centre, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada#N#
2 Kaun, Karla R.: U British Columbia, Dept of Psychology & Brain Research Ctr, Vancouver, BC, Canada
3 Rankin, Catharine H.: U British Columbia, Dept of Psychology & Brain Research Ctr, Vancouver, BC, Canada

Tóm tắt

In the experiments reported here we have developed a new group-training protocol for assessing long-term memory for habituation in Caenorhabditis elegans. We have replicated all of the major findings of the original single-worm protocol using the new protocol: (1) distributed training produced long-term retention of training, massed training did not; (2) distributed training at long interstimulus intervals (ISIs) produced long-term retention, short ISIs did not; and (3) long-term memory for distributed training is protein synthesis-dependent as it could be blocked by heat shock during the inter-block interval. In addition, we have shown that long-term memory for habituation is graded, depending on the number of blocks of stimuli in training. The inter-block interval must be >40 min for long-term retention of training to occur. Finally, we have tested long-term memory for habituation training in a strain of worms with a mutation in a vesicular glutamate transporter in the sensory neurons that transduce tap (eat-4). The results from these eat-4 worms indicate that glutamate release from the sensory neurons has an important role in the formation of long-term memory for habituation.

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Learning and Memory

Tập 9 Số 3

130-137

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