Development of Biomaterials for Gene Therapy

Molecular Therapy - Tập 2 - Trang 302-317 - 2000
Sang-oh Han1, Ram I. Mahato1, Yong Kiel Sung2, Sung Wan Kim1
1Center for Controlled Chemical Delivery (CCCD), Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, Utah, 84112
2Department of Chemistry, Dongguk University, Seoul 100-715, Korea

Tài liệu tham khảo

Ledley, 1996, Pharmaceutical approaches to somatic gene therapy, Pharm. Res., 13, 1595, 10.1023/A:1016420102549 Mahato, 1999, Pharmaceutical perspectives of nonviral gene therapy, Adv. Genet., 41, 95, 10.1016/S0065-2660(08)60152-2 Mahato, 1997, Nonviral vectors for In vivo gene therapy: Physicochemical and pharmacokinetic considerations, Crit. Rev. Ther. Drug Carrier Syst., 14, 133, 10.1615/CritRevTherDrugCarrierSyst.v14.i2.20 Smith, 1995, Viral vectors in gene therapy, Annu. Rev. Microbiol., 49, 807, 10.1146/annurev.mi.49.100195.004111 Mahato, 2000, Nonviral gene therapy: From bench to clinic, CRS Newslett, 15, 9 Rolland, 2000, From genes to gene medicines: Recent advances in nonviral gene delivery, Crit. Rev. Ther. Drug Carrier Syst., 15, 143, 10.1615/CritRevTherDrugCarrierSyst.v15.i2.20 Tang, 1997, The influence of polymer structure on the injections of cationic polymers with DNA and morphology of the resulting complexes, Gene Ther., 4, 823, 10.1038/sj.gt.3300454 Liu, 1997, Factors controlling the efficiency of cationic lipid-mediated transfection In vivo via intravenous administration, Gene Ther., 4, 517, 10.1038/sj.gt.3300424 Rakhmilevich, 1999, Gene gun-mediated IL-12 gene therapy induces antitumor effects in the absence of toxicity: A direct comparison with systemic IL-12 protein therapy, J. Immunother, 22, 135, 10.1097/00002371-199903000-00005 Felgner, 2000, Lipofectin: A highly efficient, lipid-mediated DNA transfection procedure, Proc. Natl. Acad. Sci. USA., 84, 7413, 10.1073/pnas.84.21.7413 Schwartz, 1995, Lipospermine-based gene transfer into the newborn mouse brain is optimized by a low lipospermine/DNA charge ratio, Hum. Gene Ther., 6, 1515, 10.1089/hum.1995.6.12-1515 Felgner, 1994, Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations, J. Biol. Chem., 269, 2550, 10.1016/S0021-9258(17)41980-6 McLachalan, 1994, DOTAP as a vehicle for efficient gene delivery in vitro and in vivo, Biochemistry, 11, 19 Gao, 1991, A novel cationic liposome reagent for efficient transfection of mammalian cells, Biochem. Biophys. Res. Commun., 179, 280, 10.1016/0006-291X(91)91366-K Filion, 2000, Major limitations in the use of cationic liposomes for DNA delivery, Int. J. Pharm., 162, 159, 10.1016/S0378-5173(97)00423-7 Wu, 2000, Receptor-mediated gene delivery and expression, in vivo. J. Biol. Chem., 263, 14621, 10.1016/S0021-9258(18)68081-0 Abdallah, 1996, A powerful nonviral vector for In vivo gene transfer into the adult mammalian brain: Polyethyleneimine, Hum. Gene Ther., 7, 1947, 10.1089/hum.1996.7.16-1947 Qin, 2000, Efficient transfer of genes into murine cardiac grafts by Starburst polyamidoamine dendrimers, Hum. Gene Ther., 9, 553, 10.1089/hum.1998.9.4-553 Wagner, 1990, Transferrinpolycation conjugates as carriers for DNA uptake into cells, Proc. Natl. Acad. Sci. USA., 87, 3410, 10.1073/pnas.87.9.3410 Gottschalk, 1994, Folate-receptor-mediated DNA delivery into tumor cells: Protosomal disruption results in enhanced gene expression, Gene Ther., 1, 185 Erbacher, 1999, Gene transfer with synthetic virus-like particles via the integrin-mediated endocytosis pathway, Gene Ther., 6, 138, 10.1038/sj.gt.3300783 Dash, 1993, Factors affecting blood clearance and In vivo (CRP) binding to the Sm-D protein of snRNPS. Identification of a short polypeptide binding region, Mol. Immunol., 30, 701, 10.1016/0161-5890(93)90141-W Brazeau, 2000, in vitro myotoxicity of selected cationic macromolecules used in non-viral gene delivery, Pharm. Res., 15, 680, 10.1023/A:1011954516233 Barry, 1999, Role of endogenous endonucleases and tissue site in transfection and CpG-mediated immune activation after naked DNA injection, Hum. Gene Ther., 10, 2461, 10.1089/10430349950016816 De Smedt, 2000, Cationic polymer based gene delivery systems, Pharm. Res., 17, 113, 10.1023/A:1007548826495 Trubetskoy, 1999, Layer-by-layer deposition of oppositely charged polyelectrolytes on the surface of condensed DNA particles, Nucleic Acids Res., 27, 3090, 10.1093/nar/27.15.3090 Gonsho, 1994, Tissue-targeting ability of saccharide-poly(L-lysine) conjugates, Biol. Pharm., 17, 275, 10.1248/bpb.17.275 Maruyama, 2000, Characterization of interpolyelectrolyte complexes between double-strand DNA and polylysine comb-type copolymers having hydrophilic side chains, Bioconj. Chem., 9, 292, 10.1021/bc9701510 Ferdous, 2000, Comb-type copolymer: Stabilization of triplex DNA and possible application in antigene strategy, J. Pharm. Sci., 87, 1400, 10.1021/js980066g Boussif, 1999, Synthesis of polyallylamine derivatives and their use as gene transfer vectors, in vitro. Bioconj. Chem., 10, 877, 10.1021/bc9900439 Choi, 2000, Polyethylene glycol-grafted poly-L-lysine as polymeric gene carrier, J. Control. Rel., 54, 39, 10.1016/S0168-3659(97)00174-0 Boussif, 1995, A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo:Polyethyleneimine, Proc. Natl. Acad. Sci. USA., 92, 7297, 10.1073/pnas.92.16.7297 Coll, 1999, In vivo delivery to tumors of DNA complexed with linear polyethylenimine, Hum. Gene Ther., 10, 1659, 10.1089/10430349950017662 Garnett, 1999, Gene-delivery systems using cationic polymers, Crit. Rev. Ther. Drug Carrier Syst., 16, 147, 10.1615/CritRevTherDrugCarrierSyst.v16.i2.10 Behr, 2000, Gene delivery with polycationic amphiphiles and polymers, Curr. Res. Mol. Ther., 1, 5 Lemkine, 1999, Optimization of polyethylenimine-based gene delivery to mouse brain, J. Drug Target, 7, 305, 10.3109/10611869909085513 Ogris, 1999, PEGylated DNA/transferrinPEI complexes: Reduced interaction with blood and potential for systemic gene delivery, Gene Ther., 6, 595, 10.1038/sj.gt.3300900 Nguyen, 2000, Evaluation of polyetherpolyetheneimine graft copolymers as gene transfer agents, Gene Ther., 7, 126, 10.1038/sj.gt.3301052 MacLaughlin, 2000, Chitosan and depolymerized chitosan oligomers as a condensing carriers for In vivo plasmid delivery, J. Control. Rel., 56, 259, 10.1016/S0168-3659(98)00097-2 Lee, 2000, Water soluble and low molecular weight chitosan based plasmid DNA delivery, Pharm. Res. Lee, 2000, Preparation of chitosan self-aggregates as a gene delivery system, J. Control. Rel., 51, 213, 10.1016/S0168-3659(97)00173-9 Erbacher, 2000, Chitosan-based vector/DNA complexes for gene delivery: Biophysical characteristics and transfection ability, Pharm. Res., 15, 1332, 10.1023/A:1011981000671 Roy, 1999, Oral delivery with chitosanñDNA nanoparticles generates immunologic protection in murine model of peanut allergy, Nat. Med., 5, 387, 10.1038/7385 Haensler, 1993, Polyamidoamine cascade polymers mediate efficient transfection of cells in culture, Bioconj. Chem., 4, 372, 10.1021/bc00023a012 Tang, 1996, In vitro gene delivery by degraded polyamidoamine dendrimers, Bioconj. Chem., 7, 703, 10.1021/bc9600630 Kukowska-Latallo, 1996, Efficient transfer of genetic materials into mammalian cells using starburst polyamidoamine dendrimers, Proc. Natl. Acad. Sci. USA., 93, 4897, 10.1073/pnas.93.10.4897 Maruyama-Tabata, 2000, Effective suicide gene therapy In vivo by EBV-based plasmid vector coupled with polyamidoamine dendrimer, Gene Ther., 7, 53, 10.1038/sj.gt.3301044 Zuidam, 2000, Effects of physicochemical characteristics of poly[2-(dimethylamino)ethyl methacrylate]-based polyplexes on cellular association and internalization, J. Drug Target, 8, 51, 10.3109/10611860009009209 Arigita, 1999, Association and dissociation characteristics of polymer/DNA complexes used for gene delivery, Pharm. Res., 16, 1534, 10.1023/A:1015096302720 Cherng, 1996, Effect of size and serum proteins on transfection efficiency of poly[(2-dimethylamino)ethyl methacrylate]-plasmid nanoparticles, Pharm. Res., 13, 1038, 10.1023/A:1016054623543 Cherng, 1999, Long term stability of poly((2-dimethylamino)ethyl methacrylate)-based gene delivery systems, Pharm. Res., 16, 1417, 10.1023/A:1018907310472 Park, 1999, Liposome fusion induced by pH-sensitive copolymer: Poly(4-vinylpyridine-co-N,N‘-diethylaminoethyl methacrylate), Polym. Chem., 37, 2305, 10.1002/(SICI)1099-0518(19990715)37:14<2305::AID-POLA3>3.0.CO;2-5 Mumper, 2000, Plasmid delivery to muscle: Recent advances in polymer delivery systems, Adv. Drug Del. Rev., 30, 151, 10.1016/S0169-409X(97)00113-0 Mumper, 1996, Polyvinyl derivatives as novel interactive polymers for controlled gene delivery to muscle, Pharm. Res., 13, 701, 10.1023/A:1016039330870 Mumper, 2000, Protective interactive noncondensing (PINC) polymers for enhanced plasmid distribution and expression in rat skeletal muscle, J. Control. Rel., 52, 191, 10.1016/S0168-3659(97)00215-0 Bronich, 2000, Recognition of DNA topology in reactions between plasmid DNA and cationic copolymers, Polymer Preprints, 41, 1006 Astafieva, 1996, Enhancement of the polycation-mediated DNA uptake and cell transfection with Pluronic P 85 block copolymer, FEBS Lett., 389, 278, 10.1016/0014-5793(96)00607-2 Lim, 2000, Biodegradable polyester, poly[α-(4-aminobutyl)-L-glycolic acid] asa non-toxic gene carrier, Pharm. Res., 17, 811, 10.1023/A:1007552007765 Koh, 2000, Degradable polymeric carrier for the delivery of IL-10 plasmid DNA to prevent autoimmune insulitis of NOD mice, Gene Ther., 10.1038/sj.gt.3301334 Maheshwari, 2000, Biodegradable polymer-based cytokine gene delivery for cancer treatment, Mol. Ther., 2, 121, 10.1006/mthe.2000.0105 Lim, 1999, A self-destroying polycationic polymers: Biodegradable poly(4-hydroxy-L-proline ester), J. Am. Chem. Soc., 121, 5633, 10.1021/ja984012k Putnam, 1999, Poly(4-hydroxy-L-proline ester): Low-temperature polycondensation and plasmid DNA complexation, Macromolecules, 32, 3658, 10.1021/ma982004i Hsu, 1999, Comparison of process parameters for microencapsulation of plasmid DNA in poly(D,L-lactic-co-glycolic acid) microspheres, J. Drug Target., 7, 313, 10.3109/10611869909085514 Wang, 1999, Encapsulation of plasmid DNA in biodegradable poly(D,L-lactic-co-glycolic acid) microspheres as a novel approach for immunogene delivery, J. Control. Rel., 57, 9, 10.1016/S0168-3659(98)00099-6 Capan, 1999, Preparation and characterization of poly(D,L-lactide-co-glycolide) microspheres for controlled release of poly(L-lysine) complexed plasmid DNA., Pharm. Res., 16, 509, 10.1023/A:1018862827426 Maruyama, 1997, Nanoparticle DNA carrier with poly(L-lysine) grafted polysaccharide copolymer and poly(D,L-lactic acid), Bioconj. Chem., 8, 735, 10.1021/bc9701048 Gao, 1996, Potentiation of cationic liposome-mediated gene delivery by polycations, Biochemistry, 35, 1027, 10.1021/bi952436a Whitmore, 1999, LPD lipopolyplex initiates a potent cytokine response and inhibits tumor growth, Gene Ther., 6, 1867, 10.1038/sj.gt.3301026 Zhou, 1994, DNA transfection mediated by cationic liposomes containing lipopolylysine: Characterization and mechanism of action, Biochim. Biophys. Acta, 1189, 195, 10.1016/0005-2736(94)90066-3 Kiiv, 1995, Evidence of ternary complex formation by histone H1, DNAliposomes, Biochemistry, 34, 8018, 10.1021/bi00025a007 Hagstrom, 1996, Complexes of non-cationic liposome and histone H1 mediate efficient transfection of DNA without encapsulation, Biochim. Biophys. Acta, 1284, 47, 10.1016/0005-2736(96)00106-X Fritz, 1996, Gene transfer into mammalian cells using histone-condensed plasmid DNA., Hum. Gene Ther., 7, 1395, 10.1089/hum.1996.7.12-1395 Heskins, 1968, Solution properties of poly(N-isopropylacrylamide), J. Macromol. Sci. Chem., A2, 1441, 10.1080/10601326808051910 Hinrichs, 1999, Thermosensitive polymers as carriers for gene delivery, J. Control. Rel., 60, 249, 10.1016/S0168-3659(99)00075-9 Kurisawa, 2000, Transfection efficiency increases by incorporating hydrophobic monomer units into polymeric gene carriers, J. Control. Rel., 68, 1, 10.1016/S0168-3659(00)00246-7 Gao, 2000, Tissue-engineered nipple reconstruction, Plast. Reconstr. Surg., 102, 2293 Lemieux, 2000, A combination of poloxamers increases gene expression of plasmid DNA in skeletal muscle, Gene Ther., 7, 986, 10.1038/sj.gt.3301189 Bochot, 2000, Liposomes dispersed within a thermosensitive gel: A new dosage form for ocular delivery of oligonucleotides, Pharm. Res., 15, 1364, 10.1023/A:1011989202488 Jeong, 1997, Biodegradable block copolymers as injectable drug delivery systems, Nature, 388, 860, 10.1038/42218 Jeong, 2000, Drug release from biodegradable injectable thermosensitive hydrogel of PEGPLGAPEG triblock copolymers, J. Control. Rel., 63, 155, 10.1016/S0168-3659(99)00194-7 Blessing, 2000, Monomolecular collapse of plasmid DNA into stable virus-like particles, Proc. Natl. Acad. Sci. USA., 95, 1427, 10.1073/pnas.95.4.1427 Kataoka, 1996, Nanoscopic vehicles with core-shell structure for drug targeting, 96 Kwon, 1999, Soluble self-assembly block copolymers for drug delivery, Pharm. Res., 16, 597, 10.1023/A:1011991617857 Kataoka, 2000, Tailor-made block copolymer micelles and nanoparticles for drug delivery, In 2000 Prague Meetings on Macromolecules (1720 July 2000),p. ML3 Pitard, 1997, Virus-sized self-assembling lamellar complexes between plasmid DNA and cationic micelles promote gene transfer, Proc. Natl. Acad. Sci. USA., 94, 14412, 10.1073/pnas.94.26.14412 Mahato, 1999, Plasmid-based gene therapy: Opportunities and challenges knock at the millennium, J. Drug Target., 7, 241, 10.3109/10611869909085507 Pouton, 2000, Cellular interaction and fate of polycationñDNA complexes, 255 Lear, 2000, Membrane binding and conformational properties of peptides representing the NH2 terminus of influenza HA-2, J. Biol. Chem., 262, 6500, 10.1016/S0021-9258(18)48270-1 Wolfert, 2000, Chloroquine and amphipathic peptide helices show synergistic transfection, in vitro. Gene Ther., 5, 409, 10.1038/sj.gt.3300606 Chan, 1999, Enhancement of polylysine-mediated transfection by nuclear localization sequences: Polylysine does not function as a nuclear localization sequence, Hum. Gene Ther., 10, 1695, 10.1089/10430349950017699 Sebestyen, 2000, DNA vector chemistry: The covalent attachment of signal peptides to plasmid DNA., Nat. Biotechnol, 16, 80, 10.1038/nbt0198-80 Wagner, 1991, TransferrinñpolycationñDNA complexes: The effect of polycations on the structure of the complex and DNA delivery to cells, Proc. Natl. Acad. Sci. USA., 88, 4255, 10.1073/pnas.88.10.4255 Kircheis, 1999, Polycation-based DNA complexes for tumor-targeted gene delivery, in vivo. J. Gene Med., 1, 111, 10.1002/(SICI)1521-2254(199903/04)1:2<111::AID-JGM22>3.0.CO;2-Y Puls, 1999, Gene transfer and expression of a non-viral polycation-based vector in CD4+ cells, Gene Ther., 6, 1774, 10.1038/sj.gt.3301022 Nishikawa, 2000, Pharmacokinetics and In vivo gene transfer of plasmid DNA complexed with mannosylate poly(L-lysine) in mice, J. Drug Target., 8, 29, 10.3109/10611860009009207 Diebold, 1999, Efficient gene delivery into human dendritic cells by adenovirus polyethylenimine and mannose polyethylenimine transfection, Hum. Gene Ther., 10, 775, 10.1089/10430349950018535 Mahato, 1997, Physicochemical and disposition characteristics of antisense oligonucleotides complexed with glycosylated poly(L-lysine), Biochem. Pharmacol., 53, 887, 10.1016/S0006-2952(96)00880-5 Kollen, 1999, High-efficiency transfer of cystic fibrosis transmembrane conductance regulator cDNA into cystic fibrosis airway cells in culture using lactosylated polylysine as a vector, Hum. Gene Ther., 10, 615, 10.1089/10430349950018689 Reddy, 1999, Optimization of folate-conjugated liposomal vectors for folate receptor-mediated gene therapy, J. Pharm. Sci., 88, 1112, 10.1021/js990169e Leamon, 1999, Folate copolymer-mediated transfection of cultured cells, Bioconj. Chem., 10, 947, 10.1021/bc990066n Kim, 2000, Terplex DNA delivery system as a gene carrier, Pharm. Res., 15, 116, 10.1023/A:1011917224044 Harbottle, 2000, An RGD-oligolysine peptide: A prototype construct for integrin-mediated gene delivery, Hum. Gene Ther., 9, 1037, 10.1089/hum.1998.9.7-1037 Nishikawa, 2000, Targeted delivery of plasmid DNA to hepatocytes in vivo:Optimization of the pharmacokinetics of plasmid DNA/galactosylated poly(L-lysine) complexes by controlling their physicochemical properties, J. Pharmacol. Exp. Ther., 287, 408 Ferkol, 1996, Receptor-mediated gene transfer into macrophages, Proc. Natl. Acad. Sci. USA., 93, 101, 10.1073/pnas.93.1.101 Choi, 2000, Lactose-poly(ethylene glycol)-grafted poly-L-lysine as hepatoma cell-targeted gene carrier, Bioconj. Chem., 9, 708, 10.1021/bc980017v Choi, 1999, Characterization of a targeted gene carrier, lactose-polyethylene glycol-grafted poly-L-lysineits complex with plasmid DNA., Hum. Gene Ther., 10, 2657, 10.1089/10430349950016690 Kim, 1997, In vitro gene expression on smooth muscle cells using a terplex delivery system, J. Control. Rel., 47, 51, 10.1016/S0168-3659(96)01615-X Yu, 2000, Terplex DNA gene carrier system targeting to artery wall cells, J. Control. Rel. Benns, 2000, Tailoring new gene delivery designs for specific targets, J. Drug Target., 8, 1, 10.3109/10611860009009205 Suh, 2000, Anti-JL1 antibody conjugated poly(L-lysine) for targeted gene delivery to leukemia T cells, J. Control. Rel. Simões, 2000, Mechanism of gene transfer mediated by lipoplexes associated with targeting ligands or pH-sensitive peptides, Gene Ther., 6, 1798, 10.1038/sj.gt.3301015 Schoen, 1999, Gene transfer mediated by fusion protein hemagglutinin reconstituted in cationic lipid vesicles, Gene Ther., 6, 823, 10.1038/sj.gt.3300919 Mechtler, 1997, Gene transfer mediated by influenza virus peptides: The role of peptide sequences, N. J. Chem., 21, 105 Ishiguro, 1996, Interaction of fusogenic synthetic peptide with phospholipid bilayers: Orientation of the peptide α-helix and binding isotherm, Biochemistry, 35, 4976, 10.1021/bi952547+ Subbarao, 2000, PH-dependent bilayer destabilization by an amphipathic peptide, Biochemistry, 26, 2964, 10.1021/bi00385a002 Parente, 1990, Mechanism of leakage of phospholipid vesicle contents induced by the peptide GALA, Biochemistry, 29, 8720, 10.1021/bi00489a031 Mahato, 1999, Non-viral peptide-based approaches to gene delivery, J. Drug Target., 7, 249, 10.3109/10611869909085509 Wagner, 1992, Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrinpolylysineDNA complexes: Toward a synthetic virus-like gene transfer vehicle.Proc, Natl. Acad. Sci. USA., 89, 7934, 10.1073/pnas.89.17.7934 Midoux, 2000, Membrane permeabilization and efficient gene transfer by a peptide containing several histidines, Bioconj. Chem., 9, 260, 10.1021/bc9701611 Midoux, 1999, Efficient gene transfer by histidylated polylysine/pDNA complexes, Bioconj. Chem., 10, 406, 10.1021/bc9801070 Benns, 2000, pH-sensitive cationic polymer gene delivery vehicle:N-Ac-poly(L-histidine)-graft-poly(L-lysine) comb-shaped polymer, Bioconj. Chem., 11, 637, 10.1021/bc0000177 Ferrari, 1997, ExGen 500 is an efficient vector for gene delivery to lung epithelial cells in vitro, in vivo. Gene Ther., 4, 1100, 10.1038/sj.gt.3300503 Murthy, 1999, The design and synthesis of polymers for eukaryotic membrane disruption, J. Control. Rel., 61, 137, 10.1016/S0168-3659(99)00114-5 Pouton, 2000, Nuclear import of polypeptides polynucleotidesand supramolecular complexes, Adv. Drug Deliv. Rev., 34, 51, 10.1016/S0169-409X(98)00050-7 van Dijk-Wolthuis, 1999, A versatile methods for the conjugation of proteins and peptides to poly[2-(dimethylamino)ethyl methacrylate], Bioconj. Chem., 10, 687, 10.1021/bc980126+ Branden, 1999, A peptide nucleic acid-nuclear localization signal fusion that mediates nuclear transport of DNA., Nat. Biotechnol, 17, 784, 10.1038/11726 Ciolina, 1999, Coupling of nuclear localization signals to plasmid DNA and specific interaction of the conjugates with importin α, Bioconj. Chem., 10, 49, 10.1021/bc980061a Zanta, 1999, Gene delivery: A single nuclear localization signal peptide is sufficient to carry to the cell nucleus, Proc. Natl. Acad. Sci. USA., 96, 91, 10.1073/pnas.96.1.91 Shea, 1999, DNA delivery from polymer matrices for tissue engineering, Nat. Biotechnol, 17, 551, 10.1038/9853 Bonadio, 1999, Localized directplasmid gene delivery in vivo:Prolonged therapy results in reproducible tissue regeneration, Nat. Med., 5, 753, 10.1038/10473 Ochiya, 1999, New delivery system for plasmid DNA In vivo using atelocollagen as a carrier material: The Minipellet, Nat. Med., 5, 707, 10.1038/9560 Remes, 1992, Immune response in biocompatibility, Biomaterials, 13, 731, 10.1016/0142-9612(92)90010-L Plank, 1996, Activation of the complement system by synthetic DNA complexes: A potential barrier for intravenous gene delivery, Hum. Gene Ther., 7, 1437, 10.1089/hum.1996.7.12-1437 Mohammad, 1997, Roles of polymer size and ?-amino groups in polylysineplatelet interaction, Thromb. Res., 1, 193