Nucleotide sequence and mutational analysis indicate that two <i>Helicobacter pylori</i> genes encode a P‐type ATPase and a cation‐binding protein associated with copper transport

Molecular Microbiology - Tập 15 Số 1 - Trang 97-106 - 1995
Zhongming Ge1, Koji Hiratsuka2, Diane E. Taylor2
1Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Canada
2Department of Medical Micorobiology and Infectious Diseases, University of Alberta, Edmonton, Alberta, Canada T6G 2H7.

Tóm tắt

SummaryA 2.7 kb fragment of Helicobacter pylori UA802 chromosomal DNA was cloned and sequenced. Three open reading frames (designated ORF1, oRF2 and ORF3, respectively) were predicted from the DNA sequence, of which ORF1 and ORF2 appeared to be located within the same operon. The deduced 611‐amino‐acid sequence of ORF1, a P‐type ATPase (designated hpCopA), had striking homology (29‐38%) with several bacterial P‐type ATPases and contained the potential functional domains conserved in P‐type ATPases from various sources ranging from bacterial to human. A protein of 66 amino acids (designated hpCopP) encoded by ORF2 shared extensive sequence similarity with MerP, a periplasmic mercuric ion‐transporting protein, and contains the heavy metal‐binding motif. Disruption of ORF1 with a chloramphenicol‐resistance cassette (CAT) rendered the H. pylori mutants more susceptible to cupric ion than their parental strains, whereas there is no significant alternation of susceptibility to Ni2+, Cd2+ and Hg2+ between the mutants and the parental strains. The results obtained indicate that ORF1 and ORF2 comprise a cation‐transporting system which is associated with copper export out of the H. pylori cells.

Từ khóa


Tài liệu tham khảo

Barrineau P., 1984, The DNA sequence of the mercury resistance operon of the IncFll plasmid NR1, J Mol Appl Genet, 2, 601

10.1093/nar/7.6.1513

Blaser M.J., 1967, Gastric Campytobacter‐like organisms, gastritis and peptic ulcer disease, Gastroenterology, 93, 371, 10.1016/0016-5085(87)91028-6

10.1016/0092-8674(86)90269-2

Bugnoli M., 1993, Inhibition of Helicobacter pylori urease by omeprazole, Eur J Gastroenterol Hepatol, 3, 923

10.1038/ng0193-14

Covacci A., 1993, Molecular characterization of the 128‐kDa immunodominant antigen of Helicobacter pylori associated with cytoxicity and duodenal ulcer, Proc Natl Acad Sci USA, 90, 5791, 10.1073/pnas.90.12.5791

Cover T.L., 1994, Divergence of genetic sequences for the vacuolating cytoxin among Helicobacter pylori strains, J Biol Chem, 269, 10566, 10.1016/S0021-9258(17)34097-8

10.1128/jb.174.8.2466-2473.1992

Dick J.D., 1990, Helicobacter (Campylobacter) pylori: a twist on an old disease, Annu Rev Microbiol, 108, 70

10.1111/j.1348-0421.1988.tb01373.x

10.1038/290159a0

Gellini L., 1991, Enhanced clearing of Helicobacter pylori after omeprazole plus roxithromycin treatment, FEMS Microbiol Lett, 84, 235

10.1111/j.1365-2958.1993.tb01618.x

10.1016/0022-2836(92)90934-C

10.1073/pnas.81.15.4746

10.1016/0378-1119(84)90153-7

10.1128/jb.174.15.4878-4884.1992

10.1128/AAC.35.3.490

10.1016/0022-2836(82)90515-0

10.1111/j.1365-2958.1992.tb01466.x

10.1021/jm00158a001

10.1128/AAC.36.5.1133

10.1016/0378-1119(87)90047-3

Maniatis T., 1982, Molecular Cloning: A Laboratory Manual

10.1038/ng0193-20

10.1073/pnas.81.19.5975

10.1128/AAC.37.4.769

10.1056/NEJM199110173251604

10.1073/pnas.86.10.3544

10.1128/jb.171.8.4241-4247.1989

Odermatt A., 1993, Primary structure of two P‐type ATPases involved in copper homeostasis in Enterococcus hirae, J Biol Chem, 268, 12775, 10.1016/S0021-9258(18)31455-8

10.1056/NEJM199110173251603

10.1073/pnas.85.8.2444

10.1111/j.1365-2958.1993.tb00984.x

10.1016/0022-2836(80)90196-5

10.1146/annurev.mi.42.100188.003441

10.1128/MMBR.56.1.195-228.1992

10.1038/316691a0

Solioz M., 1987, Cloning of the K+‐ATPase of Streptococcus faecalis, J Biol Chem, 262, 7358

Stolte M., 1990, Elimination of Helicobacter pylori under treatment with omeprazole, Z Gastroenterol, 28, 271

10.1128/jb.175.11.3278-3288.1993

10.1146/annurev.mi.46.100192.000343

10.1128/jb.174.21.6800-6806.1992

Tummuru M.K., 1993, Cloning and expression of a high‐molecular‐mass major antigen of Helicobacter pylori, evidence to linkage to cytotoxin production, Infect Immun, 61, 1799, 10.1128/iai.61.5.1799-1809.1993

10.1016/0167-4838(93)90078-6

10.3109/00365528909091311

10.1038/ng0193-7

10.1046/j.1365-2958.1998.00770.x

10.1016/0378-1119(90)90463-2

10.1099/00221287-139-10-2485

Thông tin
Thông tin xuất bản

Molecular Microbiology

Tập 15 Số 1

97-106

Thông tin tác giả