Extraction, sulfonation and anticoagulant activity of xylan from corncob

European Food Research and Technology - Tập 240 - Trang 969-975 - 2014
Weirong Cai1,2, Qiao Chen1, Liangliang Xie1, Lei Yang1, Rufei Zhang1
1College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu, People’s Republic of China
2Research Center of Microbial Fermentation Engineering, Wuhu, People’s Republic of China

Tóm tắt

The aim of this work was to study the anticoagulant activity of sulfonated xylan. The crude corncob xylans were isolated from corncob by alkaline extraction. The water-insoluble xylans were sulfonated using chlorosulfonic acid and dimethylformamide (DMF) to generate the water-soluble sulfonated xylan (WISX-S). The best conditions for the esterification were 1:1 of chlorosulfonic acid to DMF, keep time of 6 h and temperature of 70 °C by the orthogonal analysis. Degree of substitution of esterification product was 1.54. Anticoagulant properties of WISX-S1 and WISX-S2 were compared with normal saline and positive control heparin sodium. In vitro tests demonstrated that WISX-S2 can significantly prolong APTT and TT, but not PT. The results suggest that WISX-S2 may play the key role of anticoagulation through the intrinsic pathway and common pathway. The anticoagulant activity of xylan sulfates may be associated with the degree of substitution and molecular weight within a certain range.

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