Myeloperoxidase Exocytosis from Activated Neutrophils in the Presence of Heparin
Tóm tắt
Exocytosis of myeloperoxidase (MPO) from activated neutrophils has been investigated in the presence of the anionic polysaccharide heparin. The optimal concentration of heparin (0.1 U/mL), which did not cause additional activation of cells (lack of augmentation of lysozyme exocytosis from specific and azurophilic granules), was determined. After preincubation of cells with heparin (0.1 U/mL) MPO exocytosis from neutrophils was stimulated by various activators (fMLP, PMA, plant lectins CABA and PHA-L) and was higher as compared to the effects of the activators alone. Experiments performed using MPO isolated from leukocytes have shown that heparin in the range of concentrations 0.1–50 U/mL had no effect on MPO peroxidase activity. Thus, the use of heparin at a concentration of 0.1 U/mL avoids the artifact caused by the “loss” of MPO due to its binding to neutrophils and increases the accuracy of the method of registration of degranulation of neutrophil azurophilic granules based on determination of the MPO concentration or its peroxidase activity in cell supernatants.
Tài liệu tham khảo
Klebanoff, S.J., J. Leukoc. Biol., 2005, vol. 77, no. 5, pp. 598–625. doi 10.1189/jlb.1204697
Panasenko, O.M., Gorudko, I.V., and Sokolov, A.V., Biochemistry (Moscow), 2013, vol. 78, no. 13, pp. 1466–1489. doi 10.1134/S0006297913130075
Lefkowitz, D.L., Mills, K., Morgan, D., and Lefkowitz, S.S., Proc. Soc. Exp. Biol. Med., 1992, vol. 199, no. 2, pp. 204–210. doi 10.3181/00379727-199-43348
Gorudko, I.V., Sokolov, A.V., Shamova, E.V., Grudinina, N.A., Drozd, E.S., Shishlo, L.M., Grigorieva, D.V., Bushuk, S.B., Bushuk, B.A., Chizhik, S.A., Cherenkevich, S.N., Vasilyev, V.B., and Panasenko, O.M., Biol. Open, 2013, vol. 2, no. 9, pp. 916–923. doi 10.1242/bio.20135314
Lau, D., Mollnau, H., Eiserich, J.P., Freeman, B.A., Daiber, A., Gehling, U.M., Brümmer, J., Rudolph, V., Münzel, T., Heitzer, T., Meinertz, T., and Baldus, S., Proc. Natl. Acad. Sci. USA, 2005, vol. 102, no. 2, pp. 431–436. doi 10.1073/pnas.0405193102
Gorudko, I.V., Sokolov, A.V., Shamova, E.V., Grigorieva, D.V., Mironova, E.V., Kudryavtsev, I.V., Gusev, S.A., Gusev, A.A., Chekanov, A.V., Vasilyev, V.B., Cherenkevich, S.N., Panasenko, O.M., and Timoshenko, A.V., Arch. Biochem. Biophys., 2016, vol. 591, pp. 87–97. doi 10.1016/j.abb.2015.12.007
Van der Veen, B.S., de Winther, M.P., and Heeringa, P., Antioxid. Redox Signal., 2009, vol. 11, no. 11, pp. 2899–2937. doi 10.1089/ars.2009.2538
Gorudko, I.V., Cherkalina, O.S., Sokolov, A.V., Pulina, M.O., Zakharova, E.T., Vasilyev, V.B., Cherenkevich, S.N., and Panasenko, O.M., Russ. J. Bioorg. Chem., 2009, vol. 35, no. 5, pp. 566–575. doi 10.1134/S1068162009050057
Panasenko, O.M., Mikhalchik, E.V., Gorudko, I.V., Grigorieva, D.V., Sokolov, A.V., Kostevich, V.A., Vasilyev, V.B., and Cherenkevich, S.N., Biophysics, 2016, vol. 61, no. 3, pp. 420–428. doi 10.1134/S0006350916030131.
Franck, T., Minguet, G., Delporte, C., Derochette, S., Zouaoui Boudjeltia, K., Van Antwerpen, P., Gach, O., Deby-Dupont, G., Mouithys-Mickalad, A., and Serteyn, D., Free Radic. Res., 2015, vol. 49, no. 6, pp. 790–799. doi 10.3109/10715762.2015.1027197
Klinke, A., Nussbaum, C., Kubala, L., Friedrichs, K., Rudolph, T.K., Rudolh, V., Paust, H.J., Schröder, C., Benten, D., Lau, D., Szocs, K., Furtmüller, P.G., Heeringa, P., Sydow, K., Duchstein, H.J., Ehmke, H., Schumacher, U., Meinertz, T., Sperandio, M., and Baldus, S., Blood, 2011, vol. 117, no. 4, pp. 1350–1358. doi 10.1182/blood-2010-05-284513
Grigorieva, D.V., Gorudko, I.V., Sokolov, A.V., Kostevich, V.A., Vasilyev, V.B., Cherenkevich, S.N., and Panasenko, O.M., Bull. Exper. Biol. Med., 2016, vol. 161, no. 4, pp. 495–500. doi 10.1007/s10517-016-3446-7
El Kebir, D., József, L., Pan, W., and Filep, J.G., Circ. Res., 2008, vol. 103, no. 4, pp. 352–359. doi 10.1161/01.RES.0000326772.76822.7a
Tyrrell, D.J., Horne, A.P., Holme, K.R., Preuss, J.M., and Page, C.P., Adv. Pharmacol., 1999, vol. 46, pp. 151–208. doi 10.1016/S1054-3589(08)60471-8
Boesman-Finkelstein, M., and Finkelstein, R.A., FEBS Letters, 1982, vol. 144, no. 1, pp. 1–5. doi 10.1016/0014-5793(82)80556-5
Sokolov, A.V., Ageeva, K.V., Kostevich, V.A., Berlov, M.N., Runova, O.L., Zakharova, E.T., and Vasilyev, V.B., Biochemistry (Moscow), 2010, vol. 75, no. 11, pp. 1361–1367. doi 10.1134/S0006297910110076
Sokolov, A.V., Kostevich, V.A., Zakharova, E.T., Samygina, V.R., Panasenko, O.M., and Vasilyev, V.B., Free Radic. Res., 2015, vol. 46, no. 6, pp. 800–811. doi 10.3109/10715762.2015.1005615
Baldus, S., Rudolph, V., Roiss, M., Ito, W.D., Rudolph, T.K., Eiserich, J.P., Sydow, K., Lau, D., Szöcs, K., Klinke, A., Kubala, L., Berglund, L., Schrepfer, S., Deuse, T., Haddad, M., Risius, T., Klemm, H., Reichenspurner, H.C., Meinertz, T., and Heitzer, T., Circulation, 2006, vol. 113, no. 15, pp. 1871–1878. doi 10.1161/CIRCULATIONAHA. 105.590083
Jog, N.R., Rane, M.J., Lominadze, G., Luerman, G.C., Ward, R.A., and McLeish, K.R., Am. J. Physiol. Cell Physiol., 2007, vol. 292, no. 5, pp. 1690–1700. doi 10.1152/ajpcell.00384.2006
Videm, V., Scand. J. Immunol., 1996, vol. 43, no. 3, pp. 385–390. doi 10.1046/j.1365-3083.1996.d01-57.x
Léculier, C., Couprie, N., Adeleine, P., Leitienne, P., Francina, A., and Richard, M., Thromb. Res., 1993, vol. 69, no. 6, pp. 519–531. doi 10.1016/0049-3848(93)90056-T
Killeen, R., Wait, R., Begum, S., Gray, E., and Mulloy, B., Int. J. Exp. Pathol., 2004, vol. 85, no. 4, p. A69. doi 10.1111/j.0959-9673.2004.390af.x
Abdel-Latif, D., Steward, M., Macdonald, D.L., Francis, G.A., Dinauer, M.C., and Lacy, P., Blood, 2004, vol. 104, no. 3, pp. 832–839. doi 10.1182/blood-2003-07-2624
Timoshenko, A.V., Kayser, K., and Gabius, H.J., Methods Mol. Med., 1998, vol. 9, pp. 441–445. doi 10.1385/0-89603-393-1:441
Brown, R.A., Lever, R., Jones, N.A., and Page, C.P., Br. J. Pharmacol., 2003, vol. 139, no. 4, pp. 845–853. doi 10.1038/sj.bjp.0705291