Cardiovascular parameters in rat model of chronic renal failure induced by subtotal nephrectomy

Physiological Research - Trang S81-S88 - 2010
Jitka Švíglerová1, Jitka Kuncová1, Lukáš Nalos1, Zbyněk Tonar2, Daniel Rajdl3, Milan Štengl1
1Department of Physiology, Faculty of Medicine in Plzeň, Charles University in Prague, Czech Republic,
2Department of Histology and Embryology, Faculty of Medicine in Plzeň, Charles University in Prague, Czech Republic,
3Institute of Clinical Biochemistry and Hematology, Faculty of Medicine and Teaching Hospital in Plzeň, Charles University in Prague, Czech Republic

Tóm tắt

Chronic renal failure (CRF) is associated with high incidence of cardiovascular complications. To clarify pathogenesis of CRF numerous animal models have been developed. The aim of our work was to describe methodology of subtotal surgical renal ablation in rat and to characterize some biochemical and cardiovascular parameters of this animal model. Male rats underwent 5/6 surgical nephrectomy or sham operations in two steps. The following parameters were measured on day 10 and in week 10 after the surgery: plasma concentrations of creatinine and urea, blood pressure, resting heart rate, chronotropic response to atropine and metipranol, heart ventricles weight, contraction parameters and action potential duration in the left ventricle. Increased serum concentrations of creatinine and urea, decreased creatinine clearance, polyuria and alteration of the remnant kidney tissue were found in CRF rats. Changes in cardiovascular parameters identified after subtotal nephrectomy resembled alterations of cardiovascular system in uremic patients and included hypertension, elevated resting heart rate, diminished parasympathetic cardiac tone, hypertrophy of the left ventricle associated with weakened force of contraction, prolonged contraction and relaxation and shortening of action potential duration. These data suggest that the present model can be a useful tool in the study of CRF and its cardiovascular complications.

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Physiological Research

S81-S88

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