The narK gene product participates in nitrate transport induced in Escherichia coli nitrate‐respiring cells

FEBS Letters - Tập 252 - Trang 139-143 - 1989
Sumihare Noji1, Tsutomu Nohno2, Taiichi Saito2, Shigehiko Taniguchi1
1Department of Biochemistry, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama City 700 Japan
2Department of Pharmacology, Kawasaki Medical School, 577 Matsushima, Kurashiki City 701-01, Japan

Tóm tắt

The nucleotide sequence of the Escherichia coli narK gene, which is located in the upstream region of the narCHJI operon, was determined. The narK gene encodes a very hydrophobic protein with 463 amino acid residues (M r 49 693). A narK deletion mutant, under conditions for the induction of nitrate respiration, was unable to perform nitrate transport. Loss of transport activity was recovered by transforming the mutant with a narK + plasmid. Thus, we conclude that the narK gene encodes a transmembrane protein participating in nitrate transport. In the narK promoter region, we defined a unique sequence that we designate as a ‘nitrate box’, functioning as a putative NarL‐binding site, in addition to the consensus sequence of the ‘anaero‐box’.

Tài liệu tham khảo

10.1128/mr.52.2.190-232.1988 10.1016/0006-3002(60)91562-6 10.1016/S0021-9258(19)40989-7 10.1128/jb.121.3.1111-1116.1975 10.1016/S0021-9258(17)33574-3 10.1128/jb.154.1.387-394.1983 10.1016/S0021-9258(18)47952-5 10.1016/0014-5793(84)81295-8 10.1128/jb.159.1.159-166.1984 10.1128/jb.170.4.1721-1729.1988 10.1016/S0021-9258(18)37572-0 10.1128/JB.151.2.788-799.1982 10.1093/nar/17.8.2947 10.1073/pnas.84.11.3901 10.1128/jb.169.7.3340-3349.1987 10.1128/jb.170.4.1589-1597.1988 Bullock W.O., 1987, Biotechniques, 5, 376 10.1007/BF00341679 10.1128/JB.159.3.1034-1039.1984 10.1007/BF00337857 Miller J.H., 1972, Experiments in Molecular Genetics Davis R.W., 1980, Advanced Bacterial Genetics 10.1016/0092-8674(87)90503-4 10.1016/0378-1119(82)90015-4 10.1073/pnas.80.13.3963 10.1016/0378-1119(85)90120-9 10.1093/nar/16.20.9839 10.1093/nar/17.1.135 10.1128/jb.169.10.4614-4620.1987 10.1016/S0021-9258(18)68297-3 10.1016/S0021-9258(18)68423-6 10.1016/0022-2836(82)90515-0
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FEBS Letters

Tập 252

139-143

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