The narK gene product participates in nitrate transport induced in Escherichia coli nitrate‐respiring cells

FEBS Letters - Tập 252 - Trang 139-143 - 1989
Sumihare Noji1, Tsutomu Nohno2, Taiichi Saito2, Shigehiko Taniguchi1
1Department of Biochemistry, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama City 700 Japan
2Department of Pharmacology, Kawasaki Medical School, 577 Matsushima, Kurashiki City 701-01, Japan

Tóm tắt

The nucleotide sequence of the Escherichia coli narK gene, which is located in the upstream region of the narCHJI operon, was determined. The narK gene encodes a very hydrophobic protein with 463 amino acid residues (M r 49 693). A narK deletion mutant, under conditions for the induction of nitrate respiration, was unable to perform nitrate transport. Loss of transport activity was recovered by transforming the mutant with a narK + plasmid. Thus, we conclude that the narK gene encodes a transmembrane protein participating in nitrate transport. In the narK promoter region, we defined a unique sequence that we designate as a ‘nitrate box’, functioning as a putative NarL‐binding site, in addition to the consensus sequence of the ‘anaero‐box’.


Tài liệu tham khảo

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FEBS Letters

Tập 252

139-143

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