An investigation of the growth characteristics of oil-palm (Elaeis guineensis) suspension cultures using31P NMR

Bioscience Reports - Tập 3 - Trang 1141-1148 - 1983
W. A. Hughes1, S. M. Bociek1, J. N. Barrett1, R. G. Ratcliffe2
1Unilever Research, Colworth Laboratory, Bedford, U.K.
2Agriculture Research Council, Food Research Institute, Norwich, U.K.

Tóm tắt

High-resolution31P nuclear-magnetic-resonance (NMR) spectra are reported for oil-palm (Elaeis guineensis) cells in suspension culture. The spectra are a signicant improvement on the results that have appeared for other cultures and they are comparable with the spectra of the meristematic tissue in seedling roots. The NMR technique was used in parallel with other analytical methods to investigate the growth characteristics of the suspension culture, indluding the effect of 2,4-dichlorophenoxyacetic acid.

Tài liệu tham khảo

Rubery PH, (1977) The specificity of carrier-mediated auxin transport by suspension-culture crown gall cells, Planta135, 275–283. Cleland RE (1982) The mechanism of auxin-induced proton efflux, in: Plant Growth Substances, Proc. 11th Int. Conf. (Wareing PF, ed), Academic Press, pp 23–31. Roberts JKM & Jardetzky O (1981) Monitoring of cellular metabolism by NMR Biochem. Biophys. Acta639, 53–76. Gadian DG (1982) Nuclear Magnetic Resonance and Its Application to Living Systems, Clarendon Press, Oxford. Loughman BC & Ratcliffe RG (1983) Nuclear magnetic resonance and the study of plants. Adv. Plant Nutrition1, in press. Roberts JKM, Ray PM, Wade-Jardetzky N & Jardetzky O (1980) Estimation of cytoplasmic and vacuolar pH in higher plant cells by31P NMR. Nature (London)283, 870–872. Kime MJ, Loughman BC, Ratcliffe RG & Williams RJP (1982) The application of31P nuclear magnetic resonance of higher plant tissue. 1. Detection of spectra. J. Exp. Bot.33, 656–669. Kime MJ, Loughman BC & Ratcliffe RG (1982) The application of31P nuclear magnetic resonance to higher plant tissue. 2. Detection of intracellular changes. J. Exp. Bot.33, 670–681. Guern J (1982) Application de la spectroscopie par résonance magnétique nucléaire à l'étude du pH intracellulaire chez la plant. Comptes Rendus des Sciences: Academie d'Agriculture de France68, 504–513. Martin J-B, Bligny R, Rebeille F, Douce R, Leguay J-J, Mathieu Y and Guern J (1982) A31P nuclear magnetic resonance study of intracellular pH of plant cells cultivated in liquid medium. Plant Physiol. (Lancaster)70, 1156–1161. Rebeille F, Bligny R & Douce R (1982) Regulation of Pi uptake by Acer pseudoplatanus cells. Arch. Biochem. Biophys.219, 371–378. Rebeille F, Bligny R, Martin J-B & Douce R (1983) Relationship between the cytoplasm and the vacuole phosphate pool in Acer pseudoplatanus cells. Arch. Biochem. Biophys.225, 143–148. Schibeci A, Henry RJ, Stone BA & Brownlee RTC (1983)31P NMR measurement of cytoplasmic and vacuolar pH in endosperm cells of ryegrass (Lolium multiflorum) grown in suspension culture. Biochem. International6, 837–844. Wray V, Schiel O & Berlin J (1983) High field phosphorus-31 nuclear magnetic resonance investigation of the phosphate metabolites in cell suspension cultures of Nicotiana tabacum. Z. Pflanzenphysiologie, in press. Brown R & Rickless PA (1949) A new method for the study of cell division and cell extension with some preliminary observations on the effect of temperature and of nutrients. Proc. Roy. Soc. Lond. B136, 110–125. Lee RB & Ratcliffe RG (1983) Development of an aeration system for use in plant tissue NMR experiments. J. Exp. Bot.34, 1213–1221. Lee RB & Ratcliffe RG (1983) Phosphorus nutrition and the intracellular distribution of inorganic phosphate in pea root tips: a quantitative study using31P NMR. J. Exp. Bot.34, 1222–1244. Kirkwood RC (1976) Action on respiration and intermediary metabolism, in: Herbicides, vol 1 (Audus LJ, ed) pp 443–492, Academic Press.