The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

Clinical Chemistry - Tập 55 Số 4 - Trang 611-622 - 2009
Stephen A. Bustin1, Vladimı́r Beneš2, Jeremy A. Garson3,4, Jan Hellemans5, Jim F. Huggett6, Mikael Kubista7,8, Reinhold Mueller9, Tania Nolan10, Michael W. Pfaffl11, Gregory L. Shipley12, Jo Vandesompele5, Carl T. Wittwer13,14
1Centre for Academic Surgery, Institute of Cell and Molecular Science, Barts and the London School of Medicine and Dentistry, London, UK
2Genomics Core Facility, EMBL Heidelberg, Heidelberg, Germany
3Centre for Virology, Department of Infection, University College London, London, UK
4Department of Virology, UCL Hospitals NHS Foundation Trust, London, UK
5Center For Medical Genetics, Ghent University Hospital, Ghent, Belgium
6Centre for Infectious Diseases, University College London, London, UK
7Institute of Biotechnology AS CR, Prague, Czech Republic
8TATAA Biocenter, Göteborg, Sweden
9Sequenom, San Diego, California, USA
10Sigma–Aldrich, Haverhill, UK
11Physiology Weihenstephan, Technical University Munich, Freising, Germany
12Quantitative Genomics Core Laboratory, Department of Integrative Biology and Pharmacology, University of Texas Health Science Center, Houston, Texas, USA
13ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA
14Department of Pathology, University of Utah, Salt Lake City, Utah USA

Tóm tắt

AbstractBackground: Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of sufficient experimental detail in many publications, which impedes a reader’s ability to evaluate critically the quality of the results presented or to repeat the experiments.Content: The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines target the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimental transparency. MIQE is a set of guidelines that describe the minimum information necessary for evaluating qPCR experiments. Included is a checklist to accompany the initial submission of a manuscript to the publisher. By providing all relevant experimental conditions and assay characteristics, reviewers can assess the validity of the protocols used. Full disclosure of all reagents, sequences, and analysis methods is necessary to enable other investigators to reproduce results. MIQE details should be published either in abbreviated form or as an online supplement.Summary: Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of qPCR results.

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Clinical Chemistry

Tập 55 Số 4

611-622

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