Hyper-high-sensitivity wash-free magnetoreduction assay on biomolecules using high-Tc superconducting quantum interference devices

Journal of Applied Physics - Tập 103 Số 1 - 2008
Jen-Jie Chieh1, S. Y. Yang1, Zheng Jian1, W.C. Wang1, H. E. Horng1, H. C. Yang2, Chin‐Yih Hong3
1National Taiwan Normal University Institute of Electro-Optical Science and Technology, , Taipei 116, Taiwan
2National Taiwan University Department of Physics, , Taipei 106, Taiwan
3Nan-Kai Institute of Technology Department of Mechanical Engineering, , Nantou County 542, Taiwan

Tóm tắt

In this work, we develop a platform for assaying biomolecules involving the measurement of alternating current (ac) magnetoreduction of magnetic reagent mixed with a detected sample. The magnetic reagent contains magnetic nanoparticles coated with a given kind of antibody, which associates with conjugated biomolecules. Then, the biomolecules can be quantitatively detected by measuring the ac magnetoreduction of magnetic reagent. To achieve hyper-high-sensitivity assay, a system utilizing a high-transition-temperature rf superconducting quantum interference device (SQUID) as a sensor to probe the magnetoreduction of reagent due to the association between biomolecules and magnetic nanoparticles. Examples to assay multiactive epitope, single-active epitope, and small molecules are given to demonstrate the validity of the assay platform, as well as the hyper-high sensitivity.

Từ khóa


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Thông tin
Thông tin xuất bản

Journal of Applied Physics

Tập 103 Số 1

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