Estimation of symbiotic dinitrogen fixation in alder forest by the method based on natural15N abundance
Tóm tắt
Annual N2-fixation in virgin forest ecosystems has been measured using a15N natural abundance (δ15N) procedure. This method was compared to a15N labelled fertilizer isotopic dilution method. For young alders (5–6 years old), δ15N of leaves gave results in good agreement with the isotopic dilution of fertilizer method. Since δ15N variability was expected according to plant physiology, for alder trees, leaves were collected at various heights after the end of the growing season, and, to take account of isotopic variations coming from derived inputs, δ15N of leaves of a large number of other plants in the same are were measured to give control values. Following this procedure, the δ15N method gave reliable evaluation of the nitrogen supply, by through N2-fixation, to alders, which were found to maintain high nitrogen fixing capacity in a sequence ranging from first stage of establishment of climactic formation. Moreover, the same method is reported to discriminate various origins ofAlnus glutinosa grown in natural conditions, possibly in relation to the genetic diversity of this species.
Tài liệu tham khảo
Amarger N, Mariotti A and Mariotti F 1977 Essai d'estimation du taux d'azote fixé symbiotiquement chez le lupin par le traçage isotopique naturel (15N). C.R. Acad. Sci. Paris 284, 2179–2182.
Bardin R, Domenach A M and Chalamet A 1977 Rapports isotopiques naturels de l'azote. 11. Application à la mesure de la fixation symbiotique de l'azotein situ. Rev. Ecol. Biol. Sol 14, 395–402.
Binckley D, Sollins P and McGill W B 1985 Natural abundance of nitrogen-15 as a tool for tracing alder-fixed nitrogen. Soil Sci. Soc. Am. J. 49, 444–447.
Bremner J M and Edwards A D 1965 Determination and isotope-ratio analysis of different forms of nitrogen in soil. I. Apparatus and procedure for distillation of ammonium. Soil Sci. Soc. Am. Proc. 29, 504–507.
Coté B and Camiré C 1984 Growth, nitrogen accumulation and symbiotic dinitrogen fixation in pure and mixed plantings of hybrid poplar and black alder. Plant and Soil 78, 209–220.
Danière C, Capellano A et Moiroud A 1986 Dynamique de l'azote dans un peuplement naturel d'Alnus incana (L.) Moench. Acta Oecologica. Oecol. Plant. 7, No. 2, 165–175.
Domenach A M and Chalamet A 1977 Rapports isotopiques de l'azote. I Premiers resultats: Sols de Dombes. Rev. Ecol. Biol. Sol. 14, 279–287.
Domenach A M and Chalamet A 1979 Estimation d'azote f'e par le le soja à l'aide de deux méthodes d'analyses isotopiques. C R Acad. Sci. Paris 289, 291–294.
Domenach A M, Kurdali F, Daniere C and Bardin R 1988 Determination de l'identité isotopique de l'azote fixé parFrankia associé au genre Alnus. Can. J. Bot 66, 1241–1247.
Domenach A M and Kurdali F 1989 Influence des réserves azotées sur la formation des feuilles d'Alnus glutinosa (L.) Gaernt, et ses consequences dans l'estimation de la fixation d'azote, Can. J. Bot. “In press”.
Feigenbaum S, Bielorai H, Erner Y and Dasberg S 1987 The fate of15N labelled nitrogen applied to mature citrus trees. Plant and Soil 97, 179–187.
Fried M and Middleboe V 1977 Measurement of the amount of nitrogen fixed by a legume crop. Plant and Soil 47, 713–715.
Gauthier H G, Diem H G, Dommergues Y R and Ganry F 1985 Assesment of N2 fixation byCasuarina equisetifolia inoculated withFrankia ORSO21001 using15N methods. Soil Biol. Biochem. 17, 375–379.
Ledgard S F, Simpson J R, Freney J R and Bergersen F J 1985 Field evaluation of15N techniques for estimating nitrogen fixation in legume-grass association. Aust. J. Agric. Res. 36, 247–258.
Mariotti A 1982 Apports de la géochimie isotopique à la connaissance du cycle de l'azote. Thesis, Paris VI University, 476 p.
Pierre D 1979 Variations de l'abondance naturelle de l'isotope15N dans le sol au cours de l'humification de la matière organique. Ph D. Paris VI University, 83 p.
Riga A, Van Praag H J and Brigode N 1971 Rapports isotopiques naturels de l'azote dans quelques sols forestiers et agricoles de Belgique soumis à divers traitements culturaux. Geoderma G, 213–222.
Shearer G, Duffy J, Kohl D H and Commoner B 1974 A steady state model of isotopic fractionation accompanying nitrogen transformations in soil. Soil Sci Soc. Am. Proc. 38, 315–321.
Shearer G and Kohl D H 1986 N2-fixation in field setting: Estimations based on natural15N abundance. Aust. J. Plant Physiol. 13, 699–756.
Shearer G, Kohl D H, Virginia R A, Bryan B A, Skeeters J L, Nilsen E T, Sharifi M R and Rundel P W 1983 Estimates of N2-fixation from variation in the natural abundance of15N Sonoran desert ecosystems. Oecologia (Berlin) 56, 365–373.
Steele K W, Bonish P M, Daniel R M, and O'Hara G W 1983 Effect of rhizobial strain and host plant on nitrogen isotopic fractionation in legumes. Plant Physiol. 72, 1001–1004.
Yoneyama T, Fujita K, Yoshida T, Matsumoto T, Kambayashi I and Yazaki J 1986 Variation in natural abundance of15N among plant parts and in15N/14N fractionation during N2 fixation in the legume-Rhizobia symbiotic system. Plant Cell. Physiol. 27, No. 5, 791–799.