Extension of the Rhizobium meliloti succinoglycan biosynthesis gene cluster: identification of the exsA gene encoding an ABC transporter protein, and the exsB gene which probably codes for a regulator of succinoglycan biosynthesis

Springer Science and Business Media LLC - Tập 249 Số 5 - Trang 487-497 - 1995
Anke Becker1, H. Küster1, Karsten Niehaus1, Alfred Pühler1
1Lehrstuhl fur Genetik, Fakultat fur Biologie, Universitat Bielefeld, Bielefeld, Germany

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Altschul SF, Gish W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

Aman P, McNeil M, Franzen L, Darvill AG, Albersheim P (1981) Structural elucidation using HPLC-MS and GLC-MS of the acidic polysaccharide secreted by Rhizobium meliloti strain 1021. Carbohydr Res 95:263–282

Battisti L, Lara JC, Leigh JA (1992) Specific oligosaccharide form of the Rhizobium meliloti exopolysaccharide promotes nodule invasion in alfalfa. Proc Natl Acad Sci USA 89:5625–5629

Becker A, Kleickmann A, Arnold W, Pühler A (1993a) Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted β-1,3-1,4-glucanases and ExoH resembles membrane proteins. Mol Gen Genet 238:145–154

Becker A, Kleickmann A, Arnold W, Keller M, Pühler A (1993b) Identification and analysis of the Rhizobium meliloti exoAMONP genes involved in exopolysaccharide biosynthesis and mapping of promoters located on the exoHKLAMONP fragment. Mol Gen Genet 241:367–379

Becker A, Kleickmann A, Küster H, Keller M, Arnold W, Pühler A (1993c). Analysis of the Rhizobium meliloti genes exoU, exoV, exoW, exoT and exoI involved in exopolysaccharide biosynthesis and nodule invasion: exoU and exoW probably encode glucosyltransferases. Mol Plant Microbe Interact 6:735–744

Becker A, Niehaus K, Pühler A (1995a) Low molecular weight succinoglycan is predominantly produced by Rhizobium meliloti strains carrying a mutated ExoP protein characterized by a periplasmic N-terminal and a missing C-terminal domain. Mol Microbiol 16:191–203

Becker A, Schmidt M, Jäger W, Pühler A (1995b) New gentamicin resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. Gene 162:37–39

Beringer JE (1974) R factor transfer in Rhizobium leguminosarum. J Gen Microbiol 84:188–198

Bronner D, Clarke BR, Whitfield C (1994) Identification of an ATP-binding cassette transport system required for the translocation of lipopoysaccharide O-antigen side-chains across the cytoplasmic membrane of Klebsiella pneumoniae serotype O1. Mol Microbiol 14:505–519

Buendia AM, Enenkel B, Köplin R, Niehaus K, Arnold W, Pühler A (1991) The Rhizobium meliloti exoZ/exoB fragment of megaplasmid 2: ExoB functions as a UDP-glucose-4-epimerase and ExoZ shows homology to NodX of Rhizobium leguminosarum biovar viciae strain TOM. Mol Microbiol 5:1519–1530

Bullock WC, Fernandez JM, Short JM (1987) XL1-Blue: a high effiecient plasmid transforming recA − Escherichia coli strain with beta-galactosidase selection. BioTechniques 5:376–379

Casse F, Boucher C, Julliot JS, Michel M, Dénarié J (1979) Identification and characterization of large plasmids in Rhizobium meliloti using agarose gel electrophoresis. J Bacteriol 113:229–242

Coplin DL, Cook D (1990) Molecular genetics of extracellular polysaccharide biosynthesis in vascular phytopathogenic bacteria. Mol Plant-Microbe Interact 3:271–279

Doherty D, Leigh J, Glazebrook J, Walker GC (1988) Rhizobium meliloti mutants that overproduce the R. meliloti acidic Calcofluor-binding exopolysaccharide. J Bacteriol 170:4249–4256

Eisenberg D, Schwarz E, Komaromy M, Wall R (1984) Analysis of membrane and surface protein sequences with the hydrophobic moment plot. J Mol Biol 179:125–142

Fath MJ, Kolter, R (1993) ABC transporter: bacterial exporters. Microbiol Rev 57:995–1017

Finke A, Bronner D, Nikolaev AV, Jann B, Jann K (1991) Biosynthesis of the Escherichia coli K-5 polysaccharide, a representative of group II capsular polysaccharides: polymerization in vitro and characterization of the product. J Bacteriol 173:4088–4094

Frosch M, Edwards U, Bousset K, Kraube B, Weisgerber C (1991) Evidence for a common molecular origin of the capsule gene loci in gram-negative bacteria expressing group II capsular polysaccharides. Mol Micribiol 5:1251–1263

Glucksmann MA, Reuber TL, Walker GC (1993a) Family of glycosyltransferases needed for the synthesis of succinoglycan by Rhizobium meliloti. J Bacteriol 175:7033–7044

Glucksmann MA, Reuber TL, Walker GC (1993b) Genes needed for the modification, polymerization, export, and processing of succinoglycan by Rhizobium meliloti: a model for succinoglycan biosynthesis. J Bacteriol 175:7045–7055

Henikoff S (1984) Unidirectional digestions with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359

Hynes MF, Simon R, Müller P, Niehaus K, Labes M, Pühler A (1986) The two megaplasmids of Rhizobium meliloti are involved in the effective nodulation of alfalfa. Mol Gen Genet 202:356–362

Keller M, Roxlau A, Weng WM, Schmidt M, Quandt J, Niehaus K, Jording D, Arnold W, Pühler A (1995) Molecular analysis of the Rhizobium meliloti mucR gene regulating the biosynthesis of the exopolysaccharide succinoglycan and galactoglucan. Mol Plant Microbe Interact 2:267–277

Kessler C (1992) Nonradioactive labeling and detection of biomolecules. Springer Verlag, Berlin

Köplin R, Wang G, Hötte B, Priefer U, Pühler A (1993) A 3.9 kb DNA region of Xanthomonas campestris pv. campestris that is necessary for lipopolysaccharide production encodes a set of enzymes involved in the synthesis of dTDP-rhamnose. J Bacteriol 175:7786–7792

Kroll JS, Loynds B, Brophy LM, Moxon ER (1990) The bex locus in encapsulated Haemophilus influenzae: a chromosomal region involved in capsule polysaccharide export. Mol Microbiol 4:1853–1862

Labes M, Pühler A, Simon R (1990) A new family of RSF1010-derived expression and lac fusion broad-host-range vectors for Gram-negative bacteria. Gene 89:37–46

Leigh JA, Lee CC (1988) Characterization of polysaccharides of Rhizobium meliloti exo mutants that form ineffective nodules. J Bacteriol 170:3327–3332

Leigh JA, Walker GC (1994) Exopolysaccharides of Rhizobium: synthesis, regulation and symbiotic function. Trends Genet 10:63–67

Leigh JA, Reed JW, Hanks JF, Hirsch AM, Walker GC (1987) Rhizobium meliloti mutants that fail to succinylate their Calcofluor-binding exopolysaccharide are deficient in nodule invasion. Cell 51:579–587

Long S, Reed JW, Himawan J, Walker GC (1988) Genetic analysis of a cluster of genes required for synthesis of the Calcofluor-binding exopolysaccharide of Rhizobium meliloti. J Bacteriol 170:4239–4248

Masepohl B, Klipp W, Pühler A (1988) Genetic characterization and sequence analysis of the duplicated nifA/nifB gene region of Rhodobacter capsulatus. Mol Gen Genet 212: 27–37

Meade HM, Long SR, Ruvkun GB, Brown SE, Ausubel FM (1982) Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis. J Bacteriol 149:114–122

Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

Müller P, Hynes M, Kapp D, Niehaus K, Pühler A (1988) Two classes of Rhizobium meliloti infection mutants differ in exopolysaccharide production and in coinoculation properties with modulation mutants. Mol Gen Genet 211:17–26

Müller P, Keller M, Weng WM, Quandt J, Arnold W, Pühler A (1993) Genetic analysis of the Rhizobium meliloti exoYFQ operon: ExoY is homologous to sugar transferases and ExoQ represents a transmembrane protein. Mol Plant Microbe Interact 6:55–65

Pridmore RD (1987) New and versatile cloning vectors with kanamycin-resistance markers. Gene 56:309–312

Priefer UB, Simon R, Pühler A (1985) Extension of the host range of Escherichia coli vectors by incorporation of RSF1010 replication and mobilization functions. J Bacteriol 163:324–330

Reed JW, Glazebrook J, Walker GC (1991) The exoR gene of Rhizobium melioti affects RNA levels of other exo genes but lacks homology to known transcriptional regulators. J Bacteriol 173:3789–3794

Reinhold BB, Chan SY, Reuber TL, Marra A, Walker GC, Reinhold VN (1994) Detailed structural characterization of succinoglycan, the major exopolysaccharide of Rhizobium meliloti Rm1021. J Bacteriol 176:1997–2002

Reuber TL, Walker GC (1993a) The acetyl substituent of succinoglycan is not necessary for alfalfa nodule invasion by Rhizobium meliloti Rm1021. J Bacteriol 175:3653–3655

Reuber TL, Walker GC (1993b) Biosynthesis of succinoglycan, a symbiotically important exopolysaccharide of Rhizobium meliloti. Cell 74:269–280

Rolfe BG, Gresshoff PM, Shine J (1980) Rapid screening for symbiotic mutants of Rhizobium and white clover. Plant Sci Lett 19:277–284

Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual (2nd edn) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

Schäfer A, Tauch A, Jäger W, Kalinowski J, Thierbach G, Pühler A (1994) Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145:69–73

Simon R (1984) High frequency mobilization of gram-negative bacterial replicons by the in vitro constructed Tn5-Mob transposon. Mol Gen Genet 196:413–420

Simon R, Priefer U, Pühler A (1983) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram-negative bacteria. Biotechnology 1:784–791

Smith AN, Boulnois GJ, Roberts IS (1990) Molecular analysis of Escherichia coli K5 kps locus: identification and characterization of an inner-membrane capsular polysaccharide transport system. Mol Microbiol 4:1863–1869

Staden R (1986) The current status and portability of our sequence handling software. Nucleic Acids Res 14:217–232

Staden R, McLachlan AD (1982) Codon preference and its use in identifying protein coding regions in large DNA sequences. Nucleic Acids Res 10:141–156

Stanfield SW, Ielpi L, O'Brochta D, Helinski DR, Ditta GS (1988) The ndvA gene product of Rhizobium meliloti is required for β-(1 → 2)glucan production and has homology to the ATP-binding export protein HlyB. J Bacteriol 170:3523–3530

Steenbergen SM, Vimr ER (1990) Mechanism of polysiatic acid chain elongation in Escherichia coli K1. Mol Microbiol 4:603–611

Tolmasky ME, Staneloni RJ, Ugalde RA, Leloi LF (1980) Lipid-bound sugars in Rhizobium meliloti. Arch Biochem Biophys 203:358–364

Vieira J, Messing J (1991) New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100:189–194

Vincent JM (1970) A manual for the practical study of root nodule bacteria. IBP Handbook No. 15, Oxford

Walker JE, Sarste M, Runswick MJ, Gay NJ (1982) Distantly related sequences in the α and β-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J 1:945–951

Zhan H, Leigh JA (1990) Two genes that regulate exopolysaccharide production in Rhizobium meliloti. J Bacteriol 172:5254–5259

Zimmermann J, Voss H, Schwager C, Stegemann J, Erfle H, Stucky K, Kristensen T, Ansorge W (1990) A simplified protocol for fast plasmid DNA sequencing. Nucleic Acids Res 18:1067