Angiotensin-I converting enzyme inhibitory peptide derived from the shiitake mushroom (Lentinula edodes)
Tóm tắt
Angiotensin-I converting enzyme (ACE) inhibitors are widely used to control hypertension. In this study, protein hydrolysates from shiitake mushroom were hydrolyzed to prepare ACE-inhibitory peptides. Optimum process conditions for the hydrolysis of shiitake mushrooms using Alcalase were optimized using response surface methodology. Monitoring was conducted to check the degree of hydrolysis (DH) and ACE inhibitory activity. In the results, the optimum condition with the highest DH value of 28.88% was 50.2 °C, 3-h hydrolysis time, and 1.16 enzyme/substrate ratios. The highest ACE inhibitory activity (IC50 of 0.33 μg/mL) was under 47 °C, 3 h 28 min hydrolysis time, and 0.59 enzyme/substrate ratios. The highest activity was fractionated into 5 ranges of molecular weight, and the fraction below 0.65 kDa showed the highest activity with IC50 of 0.23 μg/mL. This fraction underwent purification using RP-HPLC, meanwhile the peak which offered a retention time of about 37 min showed high ACE inhibitory activity. Mass spectrometry identified the amino acid sequence of this peak as Lys-Ile-Gly-Ser-Arg-Ser-Arg-Phe-Asp-Val-Thr (KIGSRSRFDVT), with a molecular weight of 1265.43 Da. The synthesized variant of this peptide produced an ACE inhibitory activity (IC50) of 37.14 μM. The peptide KIGSRSRFDVT was shown to serve as a non-competitive inhibitor according to the Lineweaver–Burk plot findings. A molecular docking study was performed, which showed that the peptide binding occurred at an ACE non-active site. The findings suggest that peptides derived from shiitake mushrooms could serve either as useful components in pharmaceutical products, or in functional foods for the purpose of treating hypertension.
Tài liệu tham khảo
citation_journal_title=Process Biochem; citation_title=Angiotensin I converting enzyme (ACE) inhibitory peptides from salmon; citation_author=CB Ahn, YJ Jeon, YT Kim, JY Je; citation_volume=47; citation_publication_date=2012; citation_pages=2240-2245; citation_doi=10.3390/ijms150814077; citation_id=CR1
citation_title=Introductory mycology; citation_publication_date=1996; citation_id=CR2; citation_author=CJ Alexopoulos; citation_author=CW Minis; citation_author=M Blackwell; citation_publisher=Wiley
citation_journal_title=J Food Drug Anal; citation_title=Biochemical characterization of a novel antioxidant and angiotensin I-converting enzyme inhibitory peptide from Struthio camelus egg white protein hydrolysis; citation_author=A Asoodeh, MH Tabrizi, H Shabestarian, S Emtenani, S Emtenani; citation_volume=24; citation_publication_date=2016; citation_pages=332-342; citation_doi=10.1016/j.jfda.2015.11.010; citation_id=CR3
citation_journal_title=Mar Drugs; citation_title=Optimization of bromelain-aided production of angiotensin I-coverting enzyme inhibitory hydrolysates from stone fish using response surface methodology; citation_author=SM Auwal, M Zarei, A Abdul-Hamid, N Saari; citation_volume=15; citation_publication_date=2017; citation_pages=104; citation_doi=10.3390/md15040104; citation_id=CR4
citation_journal_title=Food Res Int; citation_title=Three novel angiotensin I-converting enzyme (ACE) inhibitory peptides from cuttlefish (Sepia officinalis) using digestive proteases; citation_author=R Balti, N Arroume, A Bougatef, D Guillochon, M Nasri; citation_volume=43; citation_publication_date=2010; citation_pages=1136-1143; citation_doi=10.1016/j.foodres.2010.02.013; citation_id=CR5
citation_journal_title=Nat Rev Dis Prim; citation_title=Hypertension; citation_author=S Bhagani, V Kapil, MD Lobo; citation_volume=46; citation_publication_date=2018; citation_pages=509-515; citation_doi=10.1016/j.mpmed.2018.06.009; citation_id=CR6
citation_journal_title=LWT Food Sci Technol; citation_title=Purification, identification, and characterization of novel angiotensin I converting enzyme (ACE) inhibitory peptides from alcalase digested horse gram flour; citation_author=B Bhaskar, L Ananthanarayan, S Jamdar; citation_volume=103; citation_publication_date=2019; citation_pages=155-161; citation_doi=10.1016/j.lwt.2018.12.059; citation_id=CR7
citation_journal_title=Curr Med Chem; citation_title=Lentinus edodes: a macrofungus with pharmacological activities; citation_author=PS Bisen, RK Baghel, BS Sanodiya, GS Thakur, GB Prasad; citation_volume=17; citation_publication_date=2010; citation_pages=2419-2430; citation_doi=10.2174/092986710791698495; citation_id=CR8
citation_journal_title=Anal Biochem; citation_title=A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding; citation_author=MM Bradford; citation_volume=72; citation_publication_date=1976; citation_pages=248-254; citation_doi=10.1016/0003-2697(76)90527-3; citation_id=CR9
citation_journal_title=J Microbiol Biotechnol; citation_title=Production and characterization of antihypertensive angiotensin I-converting enzyme inhibitor from Pholiota adipose; citation_author=KK Chul, DH Lee, JH Kim, HE Yu, JS Par, JS Lee; citation_volume=16; citation_publication_date=2006; citation_pages=757-763; citation_id=CR10
citation_journal_title=Trends Food Sci Technol; citation_title=How to trace the geographic origin of mushrooms?; citation_author=AF Sheikha, DM Hu; citation_volume=78; citation_publication_date=2018; citation_pages=292-303; citation_doi=10.1016/j.tifs.2018.06.008; citation_id=CR11
citation_journal_title=Comb Chem High Throughput Screen; citation_title=Antioxidant activity of two wild edible mushrooms (Morchella vulgaris and Morchella esculanta) from North Turkey; citation_author=M Elmastas, I Turkekul, L Ozturk, I Gulcin; citation_volume=9; citation_publication_date=2006; citation_pages=443-448; citation_doi=10.2174/138620706777698544; citation_id=CR12
citation_journal_title=J Nutr Biochem; citation_title=The possible roles of food-derived bioactive peptides in reducing the risk of cardiovascular disease; citation_author=K Erdmann, BW Cheung, H Schröder; citation_volume=19; citation_publication_date=2008; citation_pages=643-654; citation_doi=10.1016/j.jnutbio.2007.11.010; citation_id=CR13
citation_journal_title=Food Chem; citation_title=Optimisation of hydrolysis conditions for the production of the angiotensin-I converting enzyme (ACE) inhibitory peptides from whey protein using response surface methodology; citation_author=YX Guo, DD Pan, M Tanokura; citation_volume=114; citation_publication_date=2009; citation_pages=328-333; citation_doi=10.1016/j.foodchem.2008.09.041; citation_id=CR14
citation_journal_title=Molecules; citation_title=Chemical characteristics and antioxidant properties of crude water soluble polysaccharides from four common edible mushrooms; citation_author=JZ He, QM Ru, DD Dong, PL Sun; citation_volume=17; citation_publication_date=2012; citation_pages=4373-4387; citation_doi=10.3390/molecules17044373; citation_id=CR15
citation_journal_title=Conserv Biol; citation_title=Implications of phylogenetic studies for conservation of genetic diversity in Shiitake mushrooms; citation_author=DS Hibbett, MJ Donoghue; citation_volume=10; citation_publication_date=1996; citation_pages=1321-1327; citation_doi=10.1046/j.1523-1739.1996.10051321.x; citation_id=CR16
citation_journal_title=J Adv Res; citation_title=Novel angiotensin-converting enzyme inhibitory peptides from caseins and whey proteins of goat milk; citation_author=HR Ibrahim, AS Ahmed, T Miyata; citation_volume=8; citation_publication_date=2017; citation_pages=63-71; citation_doi=10.1016/j.jare.2016.12.002; citation_id=CR17
citation_journal_title=J Funct Foods; citation_title=Development and biological activities of marine-derived bioactive peptides: a review; citation_author=SK Kim, I Wijesekara; citation_volume=2; citation_publication_date=2010; citation_pages=1-9; citation_doi=10.1016/j.jff.2010.01.003; citation_id=CR18
citation_journal_title=Process Biochem; citation_title=Purification and characterization of angiotensin I-converting enzyme inhibitory peptide from enzymatic hydrolysates of Styela clava flesh tissue; citation_author=SC Ko, JK Lee, HG Byun, SC Lee, YJ Jeon; citation_volume=47; citation_publication_date=2012; citation_pages=34-40; citation_doi=10.1016/j.procbio.2011.10.005; citation_id=CR19
citation_title=How to identify mushrooms to genus, III. Microscopic features; citation_publication_date=1977; citation_id=CR20; citation_author=DL Largent; citation_author=D Johnson; citation_author=R Watling; citation_publisher=Mad River Press
citation_journal_title=Food Chem; citation_title=Effect of angiotensin I converting enzyme inhibitory peptide purified from skate skin hydrolysate; citation_author=JK Lee, JK Jeon, HG Byun; citation_volume=125; citation_publication_date=2011; citation_pages=495-499; citation_doi=10.1016/j.foodchem.2010.09.039; citation_id=CR21
citation_journal_title=Nutr Res; citation_title=Shrestha S, Angiotensin I-converting enzyme inhibitory peptides derived from food proteins and their physiological and pharmacological effects; citation_author=GH Li, GW Le, YH Shi; citation_volume=24; citation_publication_date=2004; citation_pages=469-486; citation_doi=10.1016/S0271-5317(04)00058-2; citation_id=CR22
citation_journal_title=Eur J Clin Nutr; citation_title=Bioactive peptides and proteins from foods: indication for health effects; citation_author=NP Moller, KE Scholz-Ahrens, N Roos, J Schrezenmeir; citation_volume=47; citation_publication_date=2008; citation_pages=171-182; citation_doi=10.1007/s00394-008-0710-2; citation_id=CR23
citation_journal_title=Phytomedicine; citation_title=Identification and in silico characterization of a novel peptide inhibitor of angiotensin converting enzyme from pigeon pea (Cajanus cajan); citation_author=KA Nawaz, SM David, E Murugesh, M Thandeeswaran, KG Kiran, R Mahendran, M Palaniswamy, J Angayarkanni; citation_volume=36; citation_publication_date=2017; citation_pages=1-7; citation_doi=10.1016/j.phymed.2017.09.013; citation_id=CR24
citation_journal_title=J Food Sci; citation_title=Improved method for determining food protein degree of hydrolysis; citation_author=PM Nielsen, D Petersen, C Dambmann; citation_volume=66; citation_publication_date=2001; citation_pages=642-646; citation_doi=10.1111/j.1365-2621.2001.tb04614.x; citation_id=CR25
citation_journal_title=Trends Food Sci Technol; citation_title=Metabolomics as a tool to study the mechanism of action of bioactive protein hydrolysates and peptides: a review of current literature; citation_author=JO Onuh, RE Aluko; citation_volume=91; citation_publication_date=2019; citation_pages=625-633; citation_doi=10.1016/j.tifs.2019.08.002; citation_id=CR26
citation_journal_title=Food Chem; citation_title=Studies on purification and the molecular mechanism of a novel ACE inhibitory peptide from whey protein hydrolysate; citation_author=D Pan, J Cao, H Guo, B Zhao; citation_volume=130; citation_publication_date=2012; citation_pages=121-126; citation_doi=10.1016/j.foodchem.2011.07.011; citation_id=CR27
citation_journal_title=Food Chem; citation_title=Purification and characterisation of a novel angiotensin-I converting enzyme (ACE)-inhibitory peptide derived from the enzymatic hydrolysate of Enteromorpha clathrata protein; citation_author=S Pan, S Wang, L Jing, D Yao; citation_volume=211; citation_publication_date=2016; citation_pages=423-430; citation_doi=10.1016/j.foodchem.2016.05.087; citation_id=CR28
citation_journal_title=Saudi J Biol Sci; citation_title=Lentinula edodes (shiitake mushroom): an assessment of in vitro anti-atherosclerotic bio-functionality; citation_author=MA Rahman, N Abdullah, N Aminudin; citation_volume=25; citation_publication_date=2018; citation_pages=1515-1523; citation_doi=10.1016/j.sjbs.2016.01.021; citation_id=CR29
citation_journal_title=LWT Food Sci Technol; citation_title=Isolation and characterization of nutraceutically potential ACE Inhibitory peptides from leatherjacket (Meuchenia sp.) protein hydrolysates; citation_author=J Salampessy, N Reddy, M Phillps, K Kailasapathy; citation_volume=80; citation_publication_date=2017; citation_pages=430-436; citation_doi=10.1016/j.lwt.2017.03.004; citation_id=CR30
citation_journal_title=Cardiol Clin; citation_title=The renin-angiotensin-aldosterone system and heart failure; citation_author=G Sayer, G Bhat; citation_volume=32; citation_publication_date=2014; citation_pages=21-32; citation_doi=10.1016/j.ccl.2013.09.002; citation_id=CR31
citation_journal_title=Food Chem; citation_title=Antioxidant properties and cytotoxicity of crude polysaccharides from Lentinus polychrous Lév; citation_author=C Thetsrimuang, S Khammuang, K Chiablaem, C Srisomsap, R Sarnthima; citation_volume=128; citation_publication_date=2011; citation_pages=634-639; citation_doi=10.1016/j.foodchem.2011.03.077; citation_id=CR32
citation_journal_title=LWT Food Sci Technol; citation_title=Investigating the effect of roasting on functional properties of defatted hazelnut flour by response surface methodology (RSM); citation_author=D Turan, E Capanoglu, F Altay; citation_volume=63; citation_publication_date=2015; citation_pages=758-765; citation_doi=10.1016/j.lwt.2015.03.061; citation_id=CR33
citation_journal_title=LWT Food Sci Technol; citation_title=A novel antioxidant and ACE inhibitory peptide from rice bran protein: biochemical characterization and molecular docking study; citation_author=X Wang, H Chen, X Fu, S Li, J Wei; citation_volume=75; citation_publication_date=2017; citation_pages=93-99; citation_doi=10.1016/j.lwt.2016.08.047; citation_id=CR34
citation_journal_title=Mar Drugs; citation_title=Optimization of hydrolysis conditions for the production of angiotensin I-converting enzyme inhibitory peptides and isolation of a novel peptide from lizard fish (Saurida elongata) muscle protein hydrolysate; citation_author=SG Wu, JH Sun, ZF Tong, XD Lan, ZX Zhao, DK Liao; citation_volume=10; citation_publication_date=2012; citation_pages=1066-1080; citation_doi=10.3390/md10051066; citation_id=CR35