The T/ebp null mouse: thyroid-specific enhancer-binding protein is essential for the organogenesis of the thyroid, lung, ventral forebrain, and pituitary.

Genes and Development - Tập 10 Số 1 - Trang 60-69 - 1996
Shioko Kimura1, Yukio Hara, Thierry Pineau, Robert Barouki, Cecil H. Fox, Jerrold M. Ward, Frank J. Gonzalez
1Laboratory of Molecular Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 USA

Tóm tắt

The thyroid-specific enhancer-binding protein (T/ebp) gene was disrupted by homologous recombination in embryonic stem cells to generate mice lacking T/EBP expression. Heterozygous animals developed normally, whereas mice homozygous for the disrupted gene were born dead and lacked the lung parenchyma. Instead, they had a rudimentary bronchial tree associated with an abnormal epithelium in their pleural cavities. Furthermore, the homozygous mice had no thyroid gland but had a normal parathyroid. In addition, extensive defects were found in the brain of the homozygous mice, especially in the ventral region of the forebrain. The entire pituitary, including the anterior, intermediate, and posterior pituitary, was also missing. In situ hybridization showed that the T/ebp gene is expressed in the normal thyroid, lung bronchial epithelium, and specific areas of the forebrain during early embryogenesis. These results establish that the expression of T/EBP, a transcription factor known to control thyroid-specific gene transcription, is also essential for organogenesis of the thyroid, lung, ventral forebrain, and pituitary.

Từ khóa


Tài liệu tham khảo

1992, Functional study of the human thyroid peroxidase gene promoter., Eur. J. Biochem., 203, 467, 10.1111/j.1432-1033.1992.tb16571.x

Altman, J. and S.A. Bayer. 1995. Atlas of prenatal rat brain development. CRC Press, Boca Raton, FL.

Bayer, S.A. and J. Altman. 1995a. Neurogenesis and neuronal migration. In The rat nervous system (ed. G. Paxinos), pp. 1041–1078. Academic Press, San Diego, CA.

Bayer, S.A. and J. Altman. 1995b. Principles of neurogenesis, neuronal migration, and neural circuit formation. In The rat nervous system (ed. G. Paxinos), pp. 1079–1098. Academic Press, San Diego, CA.

10.1128/MCB.14.9.5671

Bradley, A. 1987. Production and analysis of chimaeric mice. In Teratocarcinomas and embryonic stem sells: A practical approach (ed. E.J. Robertson), pp. 113&151. IRL Press, Oxford, UK.

10.1074/jbc.270.12.6531

Capen, C.C., R.A. DeLellis, and J.T. Yarrington. 1991. Endocrine system. In Handbook of toxicologic pathology (ed. W.M. Haschek and C.G. Rousseaux), pp. 675–760. Academic Press, San Diego, CA.

1989, A thyroid-specific nuclear protein essential for tissue-specific expression of the thyroglobulin promoter., EMBO J., 8, 2537, 10.1002/j.1460-2075.1989.tb08391.x

10.1210/me.7.12.1589

10.1016/0012-1606(82)90225-1

10.1016/0012-1606(83)90065-9

10.1016/0026-0495(77)90088-9

Fox, C.H. and M. Cottler-Fox. 1993. In situ hybridization for detection of HIV RNA. In Current protocols in immunology (ed. J.E. Coligan, A.M. Kruisbeek, D.H. Margulies, E.M. Shevach, and W. Strober), pp. 12.8.1–12.8.21. Green Publishing Associates/John Wiley & Sons, New York, NY.

1992, Cell-type-specific expression of the rat thyroperoxidase promoter indicates common mechanisms for thyroid-specific gene expression., Mol. Cell. Biol., 12, 576

1990, Thyroid nuclear factor 1 (TTF-1) contains a homeodomain and displays a novel DNA binding specificity., EMBO J., 9, 3631, 10.1002/j.1460-2075.1990.tb07574.x

Kaufman, M.H. 1992. The atlas of mouse development. Academic Press, London, UK.

1990, Characterization of a thyroid-specific enhancer located 5.5 kilobase pairs upstream of the human thyroid peroxidase gene., Mol. Cell. Biol., 10, 6216

10.1073/pnas.86.20.7716

10.1093/nar/19.15.4293

1991, The transcription factor TTF-1 is expressed at the onset of thyroid and lung morphogenesis and in restricted regions of the foetal brain., Development, 113, 1093, 10.1242/dev.113.4.1093

10.1016/0092-8674(92)90611-F

1993, Nkx-2.5: A novel murine homeobox gene expressed in early heart progenitor cells and their myogenic descendants., Development, 119, 419, 10.1242/dev.119.2.419

1991, Thyroid-specific enhancer-binding protein (T/EBP): cDNA cloning, functional characterization, and structural identity with thyroid transcription factor TTF-1., Mol. Cell. Biol., 11, 4927

1996, Cell differentiation of the fetal rat anterior pituitary in vitro., Cell Tiss. Res., 170, 263

1995, The complete nucleotide sequence of the mouse thyroid-specific enhancer-binding protein (T/EBP) gene: Extensive identity of the deduced amino acid sequence with the human protein., Biochim. Biophys. Acta, 1261, 304, 10.1016/0167-4781(95)00033-D

10.1016/0896-6273(92)90291-K

Rugh, R. 1968. The mouse. Its reproduction and development. Burgess Publishing Company, Minneapolis, MN.

1927, The development of the hypophysis cerebri of the albino rat., Am. J. Anat., 41, 295

10.1210/me.8.8.1049

10.1007/BF00210884

10.1007/BF00210885

Thông tin
Thông tin xuất bản

Genes and Development

Tập 10 Số 1

60-69

Thông tin tác giả