A Method for Identifying Small-Molecule Aggregators Using Photonic Crystal Biosensor Microplates

SLAS Technology - Tập 14 - Trang 348-359 - 2009
Leo L. Chan1, Erich A. Lidstone2, Kristin E. Finch3, James T. Heeres4, Paul J. Hergenrother3,4, Brian T. Cunningham1,2
1Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL
2Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL
3Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL
4Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL

Tóm tắt

Small molecules identified through high-throughput screens are an essential element in pharmaceutical discovery programs. It is now recognized that a substantial fraction of small molecules exhibit aggregating behavior leading to false positive results in many screening assays, typically due to nonspecific attachment to target proteins. Therefore, the ability to efficiently identify compounds within a screening library that aggregate can streamline the screening process by eliminating unsuitable molecules from further consideration. In this work, we show that photonic crystal (PC) optical biosensor microplate technology can be used to identify and quantify small-molecule aggregation. A group of aggregators and nonaggregators were tested using the PC technology, and measurements were compared with those gathered by three alternative methods: dynamic light scattering (DLS), an α-chymotrypsin colorimetric assay, and scanning electron microscopy (SEM). The PC biosensor measurements of aggregation were confirmed by visual observation using SEM, and were in general agreement with the α-chymotrypsin assay. DLS measurements, in contrast, demonstrated inconsistent readings for many compounds that are found to form aggregates in shapes, very different from the classical spherical particles assumed in DLS modeling. As a label-free detection method, the PC biosensor aggregation assay is simple to implement and provides a quantitative direct measurement of the mass density of material adsorbed to the transducer surface, whereas the microplate-based sensor format enables compatibility with high-throughput automated liquid-handling methods used in pharmaceutical screening.

Tài liệu tham khảo

10.1021/jm0340896 10.1038/nchembio718 10.1038/nprot.2006.77 10.1016/S0169-409X(96)00423-1 10.1016/S1367-5931(99)80058-1 10.1021/jm015507e 10.1021/jm700952v 10.1016/S1359-6446(97)01083-0 10.1038/nrd1063 10.1021/jm049131r 10.1021/jm010533y 10.1021/jm020427b 10.1016/j.drudis.2006.06.016 10.1021/jm701583b 10.1002/ppsc.200601035 International Organization for Standardization (ISO), 1996, Particle Size Analysis 10.1002/(SICI)1099-1352(199909/10)12:5<279::AID-JMR473>3.0.CO;2-3 10.1016/j.ab.2006.08.021 10.1016/j.ddtec.2004.09.009 10.1016/j.snb.2005.04.022 10.1177/1087057104267604 10.1586/14789450.3.3.271 10.1016/j.snb.2005.04.022 10.1007/s10495-006-0031-y Chan L. L., 2008, Am. Chem. Soc. Chem. Biol., 3, 437 10.1016/j.cbpa.2006.04.005
Thông tin
Thông tin xuất bản

SLAS Technology

Tập 14

348-359

Thông tin tác giả